Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 10 July 1998:
Vol. 281. no. 5374, pp. 253 - 256
DOI: 10.1126/science.281.5374.253
Prev | Table of Contents | Next

Reports

Design of a 20-Amino Acid, Three-Stranded beta -Sheet Protein

Tanja Kortemme, *dagger Marina Ramírez-Alvarado, *dagger Luis Serrano

A 20-residue protein (named Betanova) forming a monomeric, three-stranded, antiparallel beta  sheet was designed using a structural backbone template and an iterative hierarchical approach. Structural and physicochemical characterization show that the beta -sheet conformation is stabilized by specific tertiary interactions and that the protein exhibits a cooperative two-state folding-unfolding transition, which is a hallmark of natural proteins. The Betanova molecule constitutes a tractable model system to aid in the understanding of beta -sheet formation, including beta -sheet aggregation and amyloid fibril formation.

European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, Heidelberg D-69117, Germany.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed. E-mail: Kortemme{at}EMBL-Heidelberg.DE (T.K.) and Ramirez{at}EMBL-Heidelberg.DE (M.R.-A.).

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
PEP-FOLD: an online resource for de novo peptide structure prediction.
J. Maupetit, P. Derreumaux, and P. Tuffery (2009)
Nucleic Acids Res. 37, W498-W503
   Abstract »    Full Text »    PDF »
Using diastereopeptides to control metal ion coordination in proteins.
A. F. A. Peacock, L. Hemmingsen, and V. L. Pecoraro (2008)
PNAS 105, 16566-16571
   Abstract »    Full Text »    PDF »
High-resolution design of a protein loop.
X. Hu, H. Wang, H. Ke, and B. Kuhlman (2007)
PNAS 104, 17668-17673
   Abstract »    Full Text »    PDF »
Application of the multiensemble sampling to the equilibrium folding of proteins.
H. S. Son, S.-Y. Kim, J. Lee, and K.-K. Han (2006)
Bioinformatics 22, 1832-1837
   Abstract »    Full Text »    PDF »
Mean curvature as a major determinant of {beta}-sheet propensity.
E. Koh, T. Kim, and H.-s. Cho (2006)
Bioinformatics 22, 297-302
   Abstract »    Full Text »    PDF »
X-ray structure analysis of a designed oligomeric miniprotein reveals a discrete quaternary architecture.
M. H. Ali, E. Peisach, K. N. Allen, and B. Imperiali (2004)
PNAS 101, 12183-12188
   Abstract »    Full Text »    PDF »
Computational design of receptors for an organophosphate surrogate of the nerve agent soman.
M. Allert, S. S. Rizk, L. L. Looger, and H. W. Hellinga (2004)
PNAS 101, 7907-7912
   Abstract »    Full Text »    PDF »
Probabilistic approach to the design of symmetric protein quaternary structures.
X. Fu, H. Kono, and J. G. Saven (2003)
Protein Eng. Des. Sel. 16, 971-977
   Abstract »    Full Text »    PDF »
Designing proteins to crystallize through {beta}-strand pairing.
C. Wingren, A. B. Edmundson, and C. A.K. Borrebaeck (2003)
Protein Eng. Des. Sel. 16, 255-264
   Abstract »    Full Text »    PDF »
The structural basis for biphasic kinetics in the folding of the WW domain from a formin-binding protein: Lessons for protein design?.
J. Karanicolas and C. L. Brooks III (2003)
PNAS 100, 3954-3959
   Abstract »    Full Text »    PDF »
Mechanism by which 2,2,2-trifluoroethanol/water mixtures stabilize secondary-structure formation in peptides: A molecular dynamics study.
D. Roccatano, G. Colombo, M. Fioroni, and A. E. Mark (2002)
PNAS 99, 12179-12184
   Abstract »    Full Text »    PDF »
Designability of alpha -helical proteins.
E. G. Emberly, N. S. Wingreen, and C. Tang (2002)
PNAS 99, 11163-11168
   Abstract »    Full Text »    PDF »
Rationally designed mutations convert de novo amyloid-like fibrils into monomeric beta -sheet proteins.
W. Wang and M. H. Hecht (2002)
PNAS 99, 2760-2765
   Abstract »    Full Text »    PDF »
Length-dependent stability and strand length limits in antiparallel beta -sheet secondary structure.
H. E. Stanger, F. A. Syud, J. F. Espinosa, I. Giriat, T. Muir, and S. H. Gellman (2001)
PNAS
   Abstract »    Full Text »    PDF »
Tryptophan zippers: Stable, monomeric beta -hairpins.
A. G. Cochran, N. J. Skelton, and M. A. Starovasnik (2001)
PNAS
   Abstract »    Full Text »
Recent improvements in prediction of protein structure by global optimization of a potential energy function.
J. Pillardy, C. Czaplewski, A. Liwo, J. Lee, D. R. Ripoll, R. Ka, S. Oldziej, W. J. Wedemeyer, K. D. Gibson, Y. A. Arnautova, et al. (2001)
PNAS
   Abstract »    Full Text »
De novo design and characterization of an apolar helical hairpin peptide at atomic resolution: Compaction mediated by weak interactions.
U. A. Ramagopal, S. Ramakumar, D. Sahal, and V. S. Chauhan (2001)
PNAS 98, 870-874
   Abstract »    Full Text »    PDF »
Analysis of PDZ Domain-Ligand Interactions Using Carboxyl-terminal Phage Display.
G. Fuh, M. T. Pisabarro, Y. Li, C. Quan, L. A. Lasky, and S. S. Sidhu (2000)
J. Biol. Chem. 275, 21486-21491
   Abstract »    Full Text »    PDF »
Protein aging hypothesis of Alzheimer disease.
J. ORPISZEWSKI, N. SCHORMANN, B. KLUVE-BECKERMAN, J. J. LIEPNIEKS, and M. D. BENSON (2000)
FASEB J 14, 1255-1263
   Abstract »    Full Text »
Solution structure and dynamics of a de novo designed three-helix bundle protein.
S. T. R. Walsh, H. Cheng, J. W. Bryson, H. Roder, and W. F. DeGrado (1999)
PNAS 96, 5486-5491
   Abstract »    Full Text »    PDF »
Recent improvements in prediction of protein structure by global optimization of a potential energy function.
J. Pillardy, C. Czaplewski, A. Liwo, J. Lee, D. R. Ripoll, R. Kazmierkiewicz, S. Oldziej, W. J. Wedemeyer, K. D. Gibson, Y. A. Arnautova, et al. (2001)
PNAS 98, 2329-2333
   Abstract »    Full Text »    PDF »
Tryptophan zippers: Stable, monomeric beta -hairpins.
A. G. Cochran, N. J. Skelton, and M. A. Starovasnik (2001)
PNAS 98, 5578-5583
   Abstract »    Full Text »    PDF »
Length-dependent stability and strand length limits in antiparallel beta -sheet secondary structure.
H. E. Stanger, F. A. Syud, J. F. Espinosa, I. Giriat, T. Muir, and S. H. Gellman (2001)
PNAS 98, 12015-12020
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)