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Science 12 June 1998:
Vol. 280. no. 5370, pp. 1757 - 1760
DOI: 10.1126/science.280.5370.1757
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Reports

Promotion of Met-tRNAiMet Binding to Ribosomes by yIF2, a Bacterial IF2 Homolog in Yeast

Sang Ki Choi, Joon H. Lee, Wendy L. Zoll, William C. Merrick, Thomas E. Dever *

Delivery of the initiator methionine transfer RNA (Met-tRNAiMet) to the ribosome is a key step in the initiation of protein synthesis. Previous results have indicated that this step is catalyzed by the structurally dissimilar translation factors in prokaryotes and eukaryotes--initiation factor 2 (IF2) and eukaryotic initiation factor 2 (eIF2), respectively. A bacterial IF2 homolog has been identified in both eukaryotes and archaea. By using a combination of molecular genetic and biochemical studies, the Saccharomyces cerevisiae IF2 homolog is shown to function in general translation initiation by promoting Met-tRNAiMet binding to ribosomes. Thus, the mechanism of protein synthesis in eukaryotes and prokaryotes is more similar than was previously realized.

S. K. Choi, J. H. Lee, T. E. Dever, Laboratory of Eukaryotic Gene Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892-2716, USA.
W. L. Zoll and W. C. Merrick, Department of Biochemistry, Case Western Reserve University, Cleveland, OH 44106, USA.
*   To whom correspondence should be addressed: E-mail: tdever{at}box-t.nih.gov

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