Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 15 May 1998:
Vol. 280. no. 5366, pp. 1046 - 1048
DOI: 10.1126/science.280.5366.1046
Prev | Table of Contents | Next

Reports

Chemical Amplification: Continuous-Flow PCR on a Chip

Martin U. Kopp, Andrew J. de Mello, Andreas Manz *

A micromachined chemical amplifier was successfully used to perform the polymerase chain reaction (PCR) in continuous flow at high speed. The device is analogous to an electronic amplifier and relies on the movement of sample through thermostated temperature zones on a glass microchip. Input and output of material (DNA) is continuous, and amplification is independent of input concentration. A 20-cycle PCR amplification of a 176-base pair fragment from the DNA gyrase gene of Neisseria gonorrhoeae was performed at various flow rates, resulting in total reaction times of 90 seconds to 18.7 minutes.

Zeneca/SmithKline Beecham Centre for Analytical Sciences, Department of Chemistry, Imperial College of Science, Technology and Medicine, London SW7 2AY, UK.
*   To whom correspondence should be addressed. E-mail: a.manz{at}ic.ac.uk

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends.
C. Zhang and D. Xing (2007)
Nucleic Acids Res. 35, 4223-4237
   Abstract »    Full Text »    PDF »
Microarray-in-a-Tube for Detection of Multiple Viruses.
Q. Liu, Y. Bai, Q. Ge, S. Zhou, T. Wen, and Z. Lu (2007)
Clin. Chem. 53, 188-194
   Abstract »    Full Text »    PDF »
Ultra fast miniaturized real-time PCR: 40 cycles in less than six minutes.
P. Neuzil, C. Zhang, J. Pipper, S. Oh, and L. Zhuo (2006)
Nucleic Acids Res. 34, e77
   Abstract »    Full Text »    PDF »
A modular microfluidic architecture for integrated biochemical analysis.
K. A. Shaikh, K. S. Ryu, E. D. Goluch, J.-M. Nam, J. Liu, C. S. Thaxton, T. N. Chiesl, A. E. Barron, Y. Lu, C. A. Mirkin, et al. (2005)
PNAS 102, 9745-9750
   Abstract »    Full Text »    PDF »
Rapid and Simple Quantification of Bacterial Cells by Using a Microfluidic Device.
C. Sakamoto, N. Yamaguchi, and M. Nasu (2005)
Appl. Envir. Microbiol. 71, 1117-1121
   Abstract »    Full Text »    PDF »
From the Cover: Using three-dimensional microfluidic networks for solving computationally hard problems.
D. T. Chiu, E. Pezzoli, H. Wu, A. D. Stroock, and G. M. Whitesides (2001)
PNAS 98, 2961-2966
   Abstract »    Full Text »    PDF »
Portable System for Microbial Sample Preparation and Oligonucleotide Microarray Analysis.
S. G. Bavykin, J. P. Akowski, V. M. Zakhariev, V. E. Barsky, A. N. Perov, and A. D. Mirzabekov (2001)
Appl. Envir. Microbiol. 67, 922-928
   Abstract »    Full Text »
Cashing in your chips: speculation on the future of diagnostic laboratories in the era of DNA chips.
(2001)
J. Med. Microbiol. 50, 111-115
   Full Text »    PDF »
Rapid-Cycle PCR for Detection and Typing of Mycoplasma pneumoniae in Clinical Specimens.
F. Kong, S. Gordon, and G. L. Gilbert (2000)
J. Clin. Microbiol. 38, 4256-4259
   Abstract »    Full Text »
Automation for Genomics, Part Two: Sequencers, Microarrays, and Future Trends.
D. Meldrum (2000)
Genome Res. 10, 1288-1303
   Abstract »    Full Text »
SURVEY AND SUMMARY: From DNA biosensors to gene chips.
J. Wang (2000)
Nucleic Acids Res. 28, 3011-3016
   Abstract »    Full Text »    PDF »
Automated one-step DNA sequencing based on nanoliter reaction volumes and capillary electrophoresis.
H.-m. Pang and E. S. Yeung (2000)
Nucleic Acids Res. 28, e73
   Abstract »    Full Text »    PDF »
Electrochemical Micromachining.
R. Schuster, V. Kirchner, P. Allongue, and G. Ertl (2000)
Science 289, 98-101
   Abstract »    Full Text »
A miniature integrated device for automated multistep genetic assays.
R. C. Anderson, X. Su, G. J. Bogdan, and J. Fenton (2000)
Nucleic Acids Res. 28, e60
   Abstract »    Full Text »    PDF »
Monolithic Microfabricated Valves and Pumps by Multilayer Soft Lithography.
M. A. Unger, H. Chou, T. Thorsen, A. Scherer, and S. R. Quake (2000)
Science 288, 113-116
   Abstract »    Full Text »
The revolution in microanalytic chemistry: a macro-opportunity for clinical nutrition1.
M. Roberts, W. Geiger, and J B. German (2000)
Am. J. Clinical Nutrition 71, 434-437
   Full Text »    PDF »
Molecular Diagnostics on Microfabricated Electrophoretic Devices: From Slab Gel- to Capillary- to Microchip-based Assays for T- and B-Cell Lymphoproliferative Disorders.
N. J. Munro, K. Snow, J. A. Kant, and J. P. Landers (1999)
Clin. Chem. 45, 1906-1917
   Abstract »    Full Text »    PDF »
Field-Effect Flow Control for Microfabricated Fluidic Networks.
R. B. Schasfoort, S. Schlautmann, J. Hendrikse, and A. van den Berg (1999)
Science 286, 942-945
   Abstract »    Full Text »
The New Biology Enters the Generalist Pediatrician's Office: Lessons from the Human Genome Project.
E. R.B. McCabe (1999)
Pediatr. Rev. 20, 314-319
   Full Text »


ADVERTISEMENT
Click Me!

ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)