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Science 8 May 1998:
Vol. 280. no. 5365, pp. 915 - 918
DOI: 10.1126/science.280.5365.915
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Reports

Identification of Non-Heme Diiron Proteins That Catalyze Triple Bond and Epoxy Group Formation

Michael Lee, Marit Lenman, Antoni Banaś, Maureen Bafor, Surinder Singh, Michael Schweizer, Ralf Nilsson, Conny Liljenberg, Anders Dahlqvist, Per-Olov Gummeson, Staffan Sjödahl, Allan Green, Sten Stymne *

Acetylenic bonds are present in more than 600 naturally occurring compounds. Plant enzymes that catalyze the formation of the Delta 12 acetylenic bond in 9-octadecen-12-ynoic acid and the Delta 12 epoxy group in 12,13-epoxy-9-octadecenoic acid were characterized, and two genes, similar in sequence, were cloned. When these complementary DNAs were expressed in Arabidopsis thaliana, the content of acetylenic or epoxidated fatty acids in the seeds increased from 0 to 25 or 15 percent, respectively. Both enzymes have characteristics similar to the membrane proteins containing non-heme iron that have histidine-rich motifs.

M. Lee, Svalöv-Weibull AB, S-268 81 Svalöv, Sweden.
M. Lenman, A. Banaś, M. Bafor, A. Dahlqvist, P. Gummeson, S. Stymne, Department of Plant Breeding Research, Swedish University of Agricultural Sciences, S-268 31 Svalöv, Sweden.
S. Singh and A. Green, Commonwealth Scientific and Industrial Research Organization (CSIRO) Plant Industry, Canberra ACT 2601, Australia.
M. Schweizer and S. Sjödahl, Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK.
R. Nilsson and C. Liljenberg, Department of Plant Physiology, University of Gothenburg, S-413 19 Gothenburg, Sweden.
*   To whom correspondence should be addressed. E-mail: sten.stymne{at}vf.slu.se

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