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Science 24 April 1998:
Vol. 280. no. 5363, pp. 590 - 592
DOI: 10.1126/science.280.5363.590
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Reports

In Situ Visualization of DNA Double-Strand Break Repair in Human Fibroblasts

Benjamin E. Nelms, * Richard S. Maser, * James F. MacKay, Max G. Lagally, John H. J. Petrini dagger

A method was developed to examine DNA repair within the intact cell. Ultrasoft x-rays were used to induce DNA double-strand breaks (DSBs) in defined subnuclear volumes of human fibroblasts and DNA repair was visualized at those sites. The DSBs remained in a fixed position during the initial stages of DNA repair, and the DSB repair protein hMre11 migrated to the sites of damage within 30 minutes. In contrast, hRad51, a human RecA homolog, did not localize at sites of DNA damage, a finding consistent with the distinct roles of these proteins in DNA repair.

B. E. Nelms, Laboratory of Genetics and Department of Medical Physics, University of Wisconsin Medical School, Madison, WI 53706, USA.
R. S. Maser and J. H. J. Petrini, Laboratory of Genetics, University of Wisconsin Medical School, Madison, WI 53706, USA.
J. F. MacKay and M. G. Lagally, Department of Materials Science and Engineering, University of Wisconsin, Madison, WI 53706, USA.
*   These authors contributed equally to this work. Names are listed in random order.

dagger    To whom correspondence should be addressed. E-mail: jpetrini{at}facstaff.wisc.edu

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Disruption of mRad50 causes embryonic stem cell lethality, abnormal embryonic development, and sensitivity to ionizing radiation.
G. Luo, M. S. Yao, C. F. Bender, M. Mills, A. R. Bladl, A. Bradley, and J. H. J. Petrini (1999)
PNAS 96, 7376-7381
   Abstract »    Full Text »    PDF »
Nbs1 potentiates ATP-driven DNA unwinding and endonuclease cleavage by the Mre11/Rad50 complex.
T. T. Paull and M. Gellert (1999)
Genes & Dev. 13, 1276-1288
   Abstract »    Full Text »
The DNA-dependent protein kinase.
G. C.M. Smith and S. P. Jackson (1999)
Genes & Dev. 13, 916-934
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Nuclear foci of mammalian recombination proteins are located at single-stranded DNA regions formed after DNA damage.
E. Raderschall, E. I. Golub, and T. Haaf (1999)
PNAS 96, 1921-1926
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The Nuclease Activity of Mre11 Is Required for Meiosis but Not for Mating Type Switching, End Joining, or Telomere Maintenance.
S. Moreau, J. R. Ferguson, and L. S. Symington (1999)
Mol. Cell. Biol. 19, 556-566
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Pathological and Physiological Double-Strand Breaks : Roles in Cancer, Aging, and the Immune System.
M. R. Lieber (1998)
Am. J. Pathol. 153, 1323-1332
   Abstract »    Full Text »    PDF »
Xrcc3 Is Required for Assembly of Rad51 Complexes in Vivo.
D. K. Bishop, U. Ear, A. Bhattacharyya, C. Calderone, M. Beckett, R. R. Weichselbaum, and A. Shinohara (1998)
J. Biol. Chem. 273, 21482-21488
   Abstract »    Full Text »    PDF »


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