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Science 24 April 1998:
Vol. 280. no. 5363, pp. 582 - 585
DOI: 10.1126/science.280.5363.582
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Dependence of Germinal Center B Cells on Expression of CD21/CD35 for Survival

Michael B. Fischer, * Siegfried Goerg, * LiMing Shen, Andrey P. Prodeus, Christopher C. Goodnow, Garnett Kelsoe, Michael C. Carroll dagger

Affinity-driven selection of B lymphocytes within germinal centers is critical for the development of high-affinity memory cells and host protection. To investigate the role of the CD21/CD35 coreceptor in B cell competition for follicular retention and survival within the germinal center, either Cr2+ or Cr2null lysozyme-specific transgenic B cells were adoptively transferred into normal mice immunized with duck (DEL) or turkey (TEL) lysozyme, which bind with different affinities. In mice injected with high-affinity turkey lysozyme, Cr2null B cells responded by follicular retention; however, they could not survive within germinal centers. This suggests that CD21 provides a signal independent of antigen that is required for survival of B cells in the germinal center.

M. B. Fischer, S. Goerg, L. Shen, A. P. Prodeus, M. C. Carroll, Department of Pathology, Harvard Medical School, Boston, MA 02115, USA.
C. C. Goodnow, Medical Genome Centre, John Curtin School of Medical Research, Australian National University, Canberra, ACT 2601, Australia.
G. Kelsoe, Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD 21201-1509, USA.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed. E-mail: mcarroll{at}warren.med.harvard.edu

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