Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 17 April 1998:
Vol. 280. no. 5362, pp. 431 - 434
DOI: 10.1126/science.280.5362.431
Prev | Table of Contents | Next

Reports

Association of the AP-3 Adaptor Complex with Clathrin

Esteban C. Dell'Angelica, Judith Klumperman, Willem Stoorvogel, Juan S. Bonifacino *

A heterotetrameric complex termed AP-3 is involved in signal-mediated protein sorting to endosomal-lysosomal organelles. AP-3 has been proposed to be a component of a nonclathrin coat. In vitro binding assays showed that mammalian AP-3 did associate with clathrin by interaction of the appendage domain of its beta 3 subunit with the amino-terminal domain of the clathrin heavy chain. The beta 3 appendage domain contained a conserved consensus motif for clathrin binding. AP-3 colocalized with clathrin in cells as observed by immunofluorescence and immunoelectron microscopy. Thus, AP-3 function in protein sorting may depend on clathrin.

E. C. Dell'Angelica and J. S. Bonifacino, Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
J. Klumperman and W. Stoorvogel, Department of Cell Biology, School of Medicine, Institute of Biomembranes, Utrecht University, 3584 CX Utrecht, The Netherlands.
*   To whom correspondence should be addressed. E-mail: juan{at}helix.nih.gov

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Clathrin Functions in the Absence of the Terminal Domain Binding Site for Adaptor-associated Clathrin-Box Motifs.
J. R. Collette, R. J. Chi, D. R. Boettner, I. M. Fernandez-Golbano, R. Plemel, A. J. Merz, M. I. Geli, L. M. Traub, and S. K. Lemmon (2009)
Mol. Biol. Cell 20, 3401-3413
   Abstract »    Full Text »    PDF »
Functional equivalence of the clathrin heavy chains CHC17 and CHC22 in endocytosis and mitosis.
F. E. Hood and S. J. Royle (2009)
J. Cell Sci. 122, 2185-2190
   Abstract »    Full Text »    PDF »
HM1.24 Is Internalized from Lipid Rafts by Clathrin-mediated Endocytosis through Interaction with {alpha}-Adaptin.
N. Masuyama, T. Kuronita, R. Tanaka, T. Muto, Y. Hirota, A. Takigawa, H. Fujita, Y. Aso, J. Amano, and Y. Tanaka (2009)
J. Biol. Chem. 284, 15927-15941
   Abstract »    Full Text »    PDF »
A Basic Patch on {alpha}-Adaptin Is Required for Binding of Human Immunodeficiency Virus Type 1 Nef and Cooperative Assembly of a CD4-Nef-AP-2 Complex.
R. Chaudhuri, R. Mattera, O. W. Lindwasser, M. S. Robinson, and J. S. Bonifacino (2009)
J. Virol. 83, 2518-2530
   Abstract »    Full Text »    PDF »
Genome-wide Analysis of AP-3-dependent Protein Transport in Yeast.
V. C. Anand, L. Daboussi, T. C. Lorenz, and G. S. Payne (2009)
Mol. Biol. Cell 20, 1592-1604
   Abstract »    Full Text »    PDF »
Hermansky-Pudlak Syndrome Protein Complexes Associate with Phosphatidylinositol 4-Kinase Type II {alpha} in Neuronal and Non-neuronal Cells.
G. Salazar, S. Zlatic, B. Craige, A. A. Peden, J. Pohl, and V. Faundez (2009)
J. Biol. Chem. 284, 1790-1802
   Abstract »    Full Text »    PDF »
{micro}2 adaptin facilitates but is not essential for synaptic vesicle recycling in Caenorhabditis elegans.
M. Gu, K. Schuske, S. Watanabe, Q. Liu, P. Baum, G. Garriga, and E. M. Jorgensen (2008)
J. Cell Biol. 183, 881-892
   Abstract »    Full Text »    PDF »
The ENTH and C-terminal domains of Dictyostelium epsin cooperate to regulate the dynamic interaction with clathrin-coated pits.
R. J. Brady, Y. Wen, and T. J. O'Halloran (2008)
J. Cell Sci. 121, 3433-3444
   Abstract »    Full Text »    PDF »
Phosphatidylinositol-4-Kinase Type II Alpha Contains an AP-3-sorting Motif and a Kinase Domain That Are Both Required for Endosome Traffic.
B. Craige, G. Salazar, and V. Faundez (2008)
Mol. Biol. Cell 19, 1415-1426
   Abstract »    Full Text »    PDF »
The subcellular localization of the Niemann-Pick Type C proteins depends on the adaptor complex AP-3.
A. C. Berger, G. Salazar, M. L. Styers, K. A. Newell-Litwa, E. Werner, R. A. Maue, A. H. Corbett, and V. Faundez (2007)
J. Cell Sci. 120, 3640-3652
   Abstract »    Full Text »    PDF »
HIV-1 Nef-induced Down-Regulation of MHC Class I Requires AP-1 and Clathrin but Not PACS-1 and Is Impeded by AP-2.
N. B. Lubben, D. A. Sahlender, A. M. Motley, P. J. Lehner, P. Benaroch, and M. S. Robinson (2007)
Mol. Biol. Cell 18, 3351-3365
   Abstract »    Full Text »    PDF »
Neuronal and non-neuronal functions of the AP-3 sorting machinery.
K. Newell-Litwa, E. Seong, M. Burmeister, and V. Faundez (2007)
J. Cell Sci. 120, 531-541
   Abstract »    Full Text »    PDF »
Comparative proteomics of clathrin-coated vesicles.
G. H.H. Borner, M. Harbour, S. Hester, K. S. Lilley, and M. S. Robinson (2006)
J. Cell Biol. 175, 571-578
   Abstract »    Full Text »    PDF »
Clathrin Required for Phosphorylation and Internalization of beta2-Adrenergic Receptor by G Protein-coupled Receptor Kinase 2 (GRK2).
S. Mangmool, T. Haga, H. Kobayashi, K.-M. Kim, H. Nakata, M. Nishida, and H. Kurose (2006)
J. Biol. Chem. 281, 31940-31949
   Abstract »    Full Text »    PDF »
BLOC-1 Interacts with BLOC-2 and the AP-3 Complex to Facilitate Protein Trafficking on Endosomes.
S. M. Di Pietro, J. M. Falcon-Perez, D. Tenza, S. R.G. Setty, M. S. Marks, G. Raposo, and E. C. Dell'Angelica (2006)
Mol. Biol. Cell 17, 4027-4038
   Abstract »    Full Text »    PDF »
Laa1p, a Conserved AP-1 Accessory Protein Important for AP-1 Localization in Yeast.
G. E. Fernandez and G. S. Payne (2006)
Mol. Biol. Cell 17, 3304-3317
   Abstract »    Full Text »    PDF »
Calcyon, a Novel Partner of Clathrin Light Chain, Stimulates Clathrin-mediated Endocytosis.
J. Xiao, R. Dai, L. Negyessy, and C. Bergson (2006)
J. Biol. Chem. 281, 15182-15193
   Abstract »    Full Text »    PDF »
Physiological Roles of Clathrin Adaptor AP Complexes: Lessons from Mutant Animals.
H. Ohno (2006)
J. Biochem. 139, 943-948
   Abstract »    Full Text »    PDF »
The Rab5 Guanine Nucleotide Exchange Factor Rabex-5 Binds Ubiquitin (Ub) and Functions as a Ub Ligase through an Atypical Ub-interacting Motif and a Zinc Finger Domain.
R. Mattera, Y. C. Tsai, A. M. Weissman, and J. S. Bonifacino (2006)
J. Biol. Chem. 281, 6874-6883
   Abstract »    Full Text »    PDF »
Individual Phosphoinositide 3-Kinase C2{alpha} Domain Activities Independently Regulate Clathrin Function.
I. Gaidarov, Y. Zhao, and J. H. Keen (2005)
J. Biol. Chem. 280, 40766-40772
   Abstract »    Full Text »    PDF »
Role of the Endocytic Machinery in the Sorting of Lysosome-associated Membrane Proteins.
K. Janvier and J. S. Bonifacino (2005)
Mol. Biol. Cell 16, 4231-4242
   Abstract »    Full Text »    PDF »
A Novel GTPase-activating Protein for ARF6 Directly Interacts with Clathrin and Regulates Clathrin-dependent Endocytosis.
K. Tanabe, T. Torii, W. Natsume, S. Braesch-Andersen, T. Watanabe, and M. Satake (2005)
Mol. Biol. Cell 16, 1617-1628
   Abstract »    Full Text »    PDF »
Hrs, a Mammalian Master Molecule in Vesicular Transport and Protein Sorting, Suppresses the Degradation of ESCRT Proteins Signal Transducing Adaptor Molecule 1 and 2.
H. Kobayashi, N. Tanaka, H. Asao, S. Miura, M. Kyuuma, K. Semura, N. Ishii, and K. Sugamura (2005)
J. Biol. Chem. 280, 10468-10477
   Abstract »    Full Text »    PDF »
Endofin recruits clathrin to early endosomes via TOM1.
L.-F. Seet and W. Hong (2005)
J. Cell Sci. 118, 575-587
   Abstract »    Full Text »    PDF »
The Endo-Lysosomal Sorting Machinery Interacts with the Intermediate Filament Cytoskeleton.
M. L. Styers, G. Salazar, R. Love, A. A. Peden, A. P. Kowalczyk, and V. Faundez (2004)
Mol. Biol. Cell 15, 5369-5382
   Abstract »    Full Text »    PDF »
In Vitro Formation of Recycling Vesicles from Endosomes Requires Adaptor Protein-1/Clathrin and Is Regulated by Rab4 and the Connector Rabaptin-5.
A. Pagano, P. Crottet, C. Prescianotto-Baschong, and M. Spiess (2004)
Mol. Biol. Cell 15, 4990-5000
   Abstract »    Full Text »    PDF »
The RCP-Rab11 Complex Regulates Endocytic Protein Sorting.
A. A. Peden, E. Schonteich, J. Chun, J. R. Junutula, R. H. Scheller, and R. Prekeris (2004)
Mol. Biol. Cell 15, 3530-3541
   Abstract »    Full Text »    PDF »
The Trihelical Bundle Subdomain of the GGA Proteins Interacts with Multiple Partners through Overlapping but Distinct Sites.
R. Mattera, R. Puertollano, W. J. Smith, and J. S. Bonifacino (2004)
J. Biol. Chem. 279, 31409-31418
   Abstract »    Full Text »    PDF »
Localization of the AP-3 adaptor complex defines a novel endosomal exit site for lysosomal membrane proteins.
A. A. Peden, V. Oorschot, B. A. Hesser, C. D. Austin, R. H. Scheller, and J. Klumperman (2004)
J. Cell Biol. 164, 1065-1076
   Abstract »    Full Text »    PDF »
The Yeast Casein Kinase Yck3p Is Palmitoylated, then Sorted to the Vacuolar Membrane with AP-3-dependent Recognition of a YXX{phi} Adaptin Sorting Signal.
B. Sun, L. Chen, W. Cao, A. F. Roth, and N. G. Davis (2004)
Mol. Biol. Cell 15, 1397-1406
   Abstract »    Full Text »    PDF »
Definition of the Consensus Motif Recognized by {gamma}-Adaptin Ear Domains.
R. Mattera, B. Ritter, S. S. Sidhu, P. S. McPherson, and J. S. Bonifacino (2004)
J. Biol. Chem. 279, 8018-8028
   Abstract »    Full Text »    PDF »
Endocytosed Cation-Independent Mannose 6-Phosphate Receptor Traffics via the Endocytic Recycling Compartment en Route to the trans-Golgi Network and a Subpopulation of Late Endosomes.
S. X. Lin, W. G. Mallet, A. Y. Huang, and F. R. Maxfield (2004)
Mol. Biol. Cell 15, 721-733
   Abstract »    Full Text »    PDF »
Molecular and Functional Characterization of Clathrin- and AP-2-binding Determinants within a Disordered Domain of Auxilin.
U. Scheele, J. Alves, R. Frank, M. Duwel, C. Kalthoff, and E. Ungewickell (2003)
J. Biol. Chem. 278, 25357-25368
   Abstract »    Full Text »    PDF »
Does clathrin pull the fission trigger?.
G. Di Paolo and P. De Camilli (2003)
PNAS 100, 4981-4983
   Full Text »    PDF »
The Synaptojanin-like Protein Inp53/Sjl3 Functions with Clathrin in a Yeast TGN-to-Endosome Pathway Distinct from the GGA Protein-dependent Pathway.
S.-A. Ha, J. Torabinejad, D. B. DeWald, M. R. Wenk, L. Lucast, P. De Camilli, R. A. Newitt, R. Aebersold, and S. F. Nothwehr (2003)
Mol. Biol. Cell 14, 1319-1333
   Abstract »    Full Text »    PDF »
STAM and Hrs Are Subunits of a Multivalent Ubiquitin-binding Complex on Early Endosomes.
K. G. Bache, C. Raiborg, A. Mehlum, and H. Stenmark (2003)
J. Biol. Chem. 278, 12513-12521
   Abstract »    Full Text »    PDF »
The autosomal recessive hypercholesterolemia (ARH) protein interfaces directly with the clathrin-coat machinery.
S. K. Mishra, S. C. Watkins, and L. M. Traub (2002)
PNAS 99, 16099-16104
   Abstract »    Full Text »    PDF »
Structural Requirements for Interactions between Leucine-sorting Signals and Clathrin-associated Adaptor Protein Complex AP3.
D. G. Rodionov, S. Honing, A. Silye, T. L. Kongsvik, K. von Figura, and O. Bakke (2002)
J. Biol. Chem. 277, 47436-47443
   Abstract »    Full Text »    PDF »
The delta subunit of AP-3 is required for efficient transport of VSV-G from the trans-Golgi network to the cell surface.
N. Nishimura, H. Plutner, K. Hahn, and W. E. Balch (2002)
PNAS 99, 6755-6760
   Abstract »    Full Text »    PDF »
Mechanism of Interaction between Leucine-based Sorting Signals from the Invariant Chain and Clathrin-associated Adaptor Protein Complexes AP1 and AP2.
T. L. Kongsvik, S. Honing, O. Bakke, and D. G. Rodionov (2002)
J. Biol. Chem. 277, 16484-16488
   Abstract »    Full Text »    PDF »
Developmental and morphological regulation of clathrin-mediated endocytosis in Trypanosoma brucei.
G. W. Morgan, C. L. Allen, T. R. Jeffries, M. Hollinshead, and M. C. Field (2002)
J. Cell Sci. 114, 2605-2615
   Abstract »    Full Text »    PDF »
beta -Arrestin/AP-2 Interaction in G Protein-coupled Receptor Internalization. IDENTIFICATION OF A beta -ARRESTIN BINDING SITE IN beta 2-ADAPTIN.
S. A. Laporte, W. E. Miller, K.-M. Kim, and M. G. Caron (2002)
J. Biol. Chem. 277, 9247-9254
   Abstract »    Full Text »    PDF »
Role of Adaptor Complex AP-3 in Targeting Wild-Type and Mutated CD63 to Lysosomes.
B. A. Rous, B. J. Reaves, G. Ihrke, J. A.G. Briggs, S. R. Gray, D. J. Stephens, G. Banting, and J. P. Luzio (2002)
Mol. Biol. Cell 13, 1071-1082
   Abstract »    Full Text »    PDF »
Structural Requirements for Function of Yeast GGAs in Vacuolar Protein Sorting, alpha -Factor Maturation, and Interactions with Clathrin.
C. Mullins and J. S. Bonifacino (2001)
Mol. Cell. Biol. 21, 7981-7994
   Abstract »    Full Text »    PDF »
Clathrin- and AP-2-binding Sites in HIP1 Uncover a General Assembly Role for Endocytic Accessory Proteins.
S. K. Mishra, N. R. Agostinelli, T. J. Brett, I. Mizukami, T. S. Ross, and L. M. Traub (2001)
J. Biol. Chem. 276, 46230-46236
   Abstract »    Full Text »    PDF »
Amphiphysin is necessary for organization of the excitation-contraction coupling machinery of muscles, but not for synaptic vesicle endocytosis in Drosophila.
A. Razzaq, I. M. Robinson, H. T. McMahon, J. N. Skepper, Y. Su, A. C. Zelhof, A. P. Jackson, N. J. Gay, and C. J. O'Kane (2001)
Genes & Dev. 15, 2967-2979
   Abstract »    Full Text »    PDF »
Insulin-regulated Release from the Endosomal Recycling Compartment Is Regulated by Budding of Specialized Vesicles.
M. A. Lampson, J. Schmoranzer, A. Zeigerer, S. M. Simon, and T. E. McGraw (2001)
Mol. Biol. Cell 12, 3489-3501
   Abstract »    Full Text »    PDF »
Golgi-localized, gamma-Ear-containing, ADP-Ribosylation Factor-binding Proteins: Roles of the Different Domains and Comparison with AP-1 and Clathrin.
J. Hirst, M. R. Lindsay, and M. S. Robinson (2001)
Mol. Biol. Cell 12, 3573-3588
   Abstract »    Full Text »    PDF »
Adaptins. The Final Recount.
M. Boehm and J. S. Bonifacino (2001)
Mol. Biol. Cell 12, 2907-2920
   Abstract »    Full Text »    PDF »
The actin-binding protein Hip1R associates with clathrin during early stages of endocytosis and promotes clathrin assembly in vitro.
A. E.Y. Engqvist-Goldstein, R. A. Warren, M. M. Kessels, J. H. Keen, J. Heuser, and D. G. Drubin (2001)
J. Cell Biol. 154, 1209-1224
   Abstract »    Full Text »    PDF »
{gamma}Subunit of the AP-1 Adaptor Complex Binds Clathrin: Implications for Cooperative Binding in Coated Vesicle Assembly.
B. Doray and S. Kornfeld (2001)
Mol. Biol. Cell 12, 1925-1935
   Abstract »    Full Text »    PDF »
AP-3 Mediates Tyrosinase but Not TRP-1 Trafficking in Human Melanocytes.
M. Huizing, R. Sarangarajan, E. Strovel, Y. Zhao, W. A. Gahl, and R. E. Boissy (2001)
Mol. Biol. Cell 12, 2075-2085
   Abstract »    Full Text »    PDF »
Yeast Gga Coat Proteins Function with Clathrin in Golgi to Endosome Transport.
G. Costaguta, C. J. Stefan, E. S. Bensen, S. D. Emr, and G. S. Payne (2001)
Mol. Biol. Cell 12, 1885-1896
   Abstract »    Full Text »    PDF »
Simultaneous Binding of PtdIns(4,5)P2 and Clathrin by AP180 in the Nucleation of Clathrin Lattices on Membranes.
M. G. J. Ford, B. M. F. Pearse, M. K. Higgins, Y. Vallis, D. J. Owen, A. Gibson, C. R. Hopkins, P. R. Evans, and H. T. McMahon (2001)
Science 291, 1051-1055
   Abstract »    Full Text »
Distribution and Function of Ap-1 Clathrin Adaptor Complexes in Polarized Epithelial Cells.
H. Folsch, M. Pypaert, P. Schu, and I. Mellman (2001)
J. Cell Biol. 152, 595-606
   Abstract »    Full Text »    PDF »
Dominant-Interfering Hsc70 Mutants Disrupt Multiple Stages of the Clathrin-Coated Vesicle Cycle in Vivo.
S. L. Newmyer and S. L. Schmid (2001)
J. Cell Biol. 152, 607-620
   Abstract »    Full Text »    PDF »
Roles of the cytoplasmic and transmembrane domains of syntaxins in intracellular localization and trafficking.
K. Kasai and K. Akagawa (2001)
J. Cell Sci. 114, 3115-3124
   Abstract »    Full Text »    PDF »
Vps41p Function in the Alkaline Phosphatase Pathway Requires Homo-oligomerization and Interaction with AP-3 through Two Distinct Domains.
T. Darsow, D. J. Katzmann, C. R. Cowles, and S. D. Emr (2001)
Mol. Biol. Cell 12, 37-51
   Abstract »    Full Text »
Molecular analysis of vesicular amine transporter function and targeting to secretory organelles.
J. D. ERICKSON and H. VAROQUI (2000)
FASEB J 14, 2450-2458
   Abstract »    Full Text »
A Conserved Clathrin Assembly Motif Essential for Synaptic Vesicle Endocytosis.
J. R. Morgan, K. Prasad, W. Hao, G. J. Augustine, and E. M. Lafer (2000)
J. Neurosci. 20, 8667-8676
   Abstract »    Full Text »    PDF »
The Assembly of AP-3 Adaptor Complex-containing Clathrin-coated Vesicles on Synthetic Liposomes.
M. T. Drake, Y. Zhu, and S. Kornfeld (2000)
Mol. Biol. Cell 11, 3723-3736
   Abstract »    Full Text »
Clathrin in mitotic spindles.
C. T. Okamoto, J. McKinney, and Y. Y. Jeng (2000)
Am J Physiol Cell Physiol 279, C369-C374
   Abstract »    Full Text »    PDF »
The AP-3 Complex Required for Endosomal Synaptic Vesicle Biogenesis is Associated with a Casein Kinase Ialpha -Like Isoform.
V. V. Faundez and R. B. Kelly (2000)
Mol. Biol. Cell 11, 2591-2604
   Abstract »    Full Text »
Lysosome-related organelles.
E. C. DELL’ANGELICA, C. MULLINS, S. CAPLAN, and J. S. BONIFACINO (2000)
FASEB J 14, 1265-1278
   Abstract »    Full Text »
Defective Pigment Granule Biogenesis and Aberrant Behavior Caused by Mutations in the Drosophila AP-3{beta} Adaptin Gene ruby.
D. Kretzschmar, B. Poeck, H. Roth, R. Ernst, A. Keller, M. Porsch, R. Strauss, and G. O. Pflugfelder (2000)
Genetics 155, 213-223
   Abstract »    Full Text »
Ggas: A Family of Adp Ribosylation Factor-Binding Proteins Related to Adaptors and Associated with the Golgi Complex.
E. C. Dell'Angelica, R. Puertollano, C. Mullins, R. C. Aguilar, J. D. Vargas, L. M. Hartnell, and J. S. Bonifacino (2000)
J. Cell Biol. 149, 81-94
   Abstract »    Full Text »    PDF »
Vear, a Novel Golgi-associated Protein with VHS and gamma -Adaptin "Ear" Domains.
A. Poussu, O. Lohi, and V.-P. Lehto (2000)
J. Biol. Chem. 275, 7176-7183
   Abstract »    Full Text »    PDF »
Epsin Binds to Clathrin by Associating Directly with the Clathrin-terminal Domain. EVIDENCE FOR COOPERATIVE BINDING THROUGH TWO DISCRETE SITES.
M. T. Drake, M. A. Downs, and L. M. Traub (2000)
J. Biol. Chem. 275, 6479-6489
   Abstract »    Full Text »    PDF »
From the Cover: Peptide-in-groove interactions link target proteins to the beta -propeller of clathrin.
E. ter Haar, S. C. Harrison, and T. Kirchhausen (2000)
PNAS 97, 1096-1100
   Abstract »    Full Text »    PDF »
Molecular Characterization of the Protein Encoded by the Hermansky-Pudlak Syndrome Type 1 Gene.
E. C. Dell'Angelica, R. C. Aguilar, N. Wolins, S. Hazelwood, W. A. Gahl, and J. S. Bonifacino (2000)
J. Biol. Chem. 275, 1300-1306
   Abstract »    Full Text »    PDF »
Bi-directional trafficking between the trans-Golgi network and the endosomal/lysosomal system.
W. Rohn, Y Rouille, S Waguri, and B Hoflack (2000)
J. Cell Sci. 113, 2093-2101
   Abstract »    PDF »
Lysosome-endosome fusion and lysosome biogenesis.
J. Luzio, B. Rous, N. Bright, P. Pryor, B. Mullock, and R. Piper (2000)
J. Cell Sci. 113, 1515-1524
   Abstract »    PDF »
Synthetic Genetic Interactions With Temperature-Sensitive Clathrin in Saccharomyces cerevisiae: Roles for Synaptojanin-Like Inp53p and Dynamin-Related Vps1p in Clathrin-Dependent Protein Sorting at the trans-Golgi Network.
E. S. Bensen, G. Costaguta, and G. S. Payne (2000)
Genetics 154, 83-97
   Abstract »    Full Text »
The Leucine-based Sorting Motifs in the Cytoplasmic Domain of the Invariant Chain Are Recognized by the Clathrin Adaptors AP1 and AP2 and their Medium Chains.
M. W. Hofmann, S. Honing, D. Rodionov, B. Dobberstein, K. von Figura, and O. Bakke (1999)
J. Biol. Chem. 274, 36153-36158
   Abstract »    Full Text »    PDF »
The Epsins Define a Family of Proteins That Interact with Components of the Clathrin Coat and Contain a New Protein Module.
J. A. Rosenthal, H. Chen, V. I. Slepnev, L. Pellegrini, A. E. Salcini, P. P. Di Fiore, and P. De Camilli (1999)
J. Biol. Chem. 274, 33959-33965
   Abstract »    Full Text »    PDF »
Adaptor Complex-independent Clathrin Function in Yeast.
B. G. Yeung, H. L. Phan, and G. S. Payne (1999)
Mol. Biol. Cell 10, 3643-3659
   Abstract »    Full Text »
AP180 and AP-2 Interact Directly in a Complex That Cooperatively Assembles Clathrin.
W. Hao, Z. Luo, L. Zheng, K. Prasad, and E. M. Lafer (1999)
J. Biol. Chem. 274, 22785-22794
   Abstract »    Full Text »    PDF »
Clathrin Assembly Lymphoid Myeloid Leukemia (CALM) Protein: Localization in Endocytic-coated Pits, Interactions with Clathrin, and the Impact of Overexpression on Clathrin-mediated Traffic.
F. Tebar, S. K. Bohlander, and A. Sorkin (1999)
Mol. Biol. Cell 10, 2687-2702
   Abstract »    Full Text »
Characterization of a Fourth Adaptor-related Protein Complex.
J. Hirst, N. A. Bright, B. Rous, and M. S. Robinson (1999)
Mol. Biol. Cell 10, 2787-2802
   Abstract »    Full Text »
The Structural Era of Endocytosis.
M. Marsh and H. T. McMahon (1999)
Science 285, 215-220
   Abstract »    Full Text »
Molecular Bases for the Recognition of Tyrosine-based Sorting Signals.
J. S. Bonifacino and E. C. Dell'Angelica (1999)
J. Cell Biol. 145, 923-926
   Full Text »    PDF »
Splice Variants of Intersectin Are Components of the Endocytic Machinery in Neurons and Nonneuronal Cells.
N. K. Hussain, M. Yamabhai, A. R. Ramjaun, A. M. Guy, D. Baranes, J. P. O'Bryan, C. J. Der, B. K. Kay, and P. S. McPherson (1999)
J. Biol. Chem. 274, 15671-15677
   Abstract »    Full Text »    PDF »
The Specificity of Vesicle Trafficking: Coat Proteins and SNAREs.
A. A. Sanderfoot and N. V. Raikhel (1999)
PLANT CELL 11, 629-642
   Full Text »
AP-4, a Novel Protein Complex Related to Clathrin Adaptors.
E. C. Dell'Angelica, C. Mullins, and J. S. Bonifacino (1999)
J. Biol. Chem. 274, 7278-7285
   Abstract »    Full Text »    PDF »
Early Embryonic Death of Mice Deficient in gamma -Adaptin.
D. Zizioli, C. Meyer, G. Guhde, P. Saftig, K. von Figura, and P. Schu (1999)
J. Biol. Chem. 274, 5385-5390
   Abstract »    Full Text »    PDF »
Interactions of the AP-1 Golgi Adaptor with the Polymeric Immunoglobulin Receptor and Their Possible Role in Mediating Brefeldin A-sensitive Basolateral Targeting from the trans-Golgi Network.
E. Orzech, K. Schlessinger, A. Weiss, C. T. Okamoto, and B. Aroeti (1999)
J. Biol. Chem. 274, 2201-2215
   Abstract »    Full Text »    PDF »
Utilization of the indirect lysosome targeting pathway by lysosome-associated membrane proteins (LAMPs) is influenced largely by the C-terminal residue of their GYXXphi targeting signals.
N. Gough, M. Zweifel, O Martinez-Augustin, R. Aguilar, J. Bonifacino, and D. Fambrough (1999)
J. Cell Sci. 112, 4257-4269
   Abstract »    PDF »
Studies on the inhibition of endosome fusion by GTPgammaS-bound ARF.
A. Jones, D. Spiro, T Kirchhausen, P Melancon, and M Wessling-Resnick (1999)
J. Cell Sci. 112, 3477-3485
   Abstract »    PDF »
Clathrin, adaptors and eps15 in endosomes containing activated epidermal growth factor receptors.
T Sorkina, A Bild, F Tebar, and A Sorkin (1999)
J. Cell Sci. 112, 317-327
   Abstract »    PDF »
The tyrosinase tail mediates sorting to the lysosomal compartment in MDCK cells via a di-leucine and a tyrosine-based signal.
T Simmen, A Schmidt, W Hunziker, and F Beermann (1999)
J. Cell Sci. 112, 45-53
   Abstract »    PDF »
New Perspectives on Mechanisms Involved in Generating Epithelial Cell Polarity.
C. YEAMAN, K. K. GRINDSTAFF, and W. J. NELSON (1999)
Physiol Rev 79, 73-98
   Abstract »    Full Text »    PDF »
Intersectin, a Novel Adaptor Protein with Two Eps15 Homology and Five Src Homology 3 Domains.
M. Yamabhai, N. G. Hoffman, N. L. Hardison, P. S. McPherson, L. Castagnoli, G. Cesareni, and B. K. Kay (1998)
J. Biol. Chem. 273, 31401-31407
   Abstract »    Full Text »    PDF »
Neuroendocrine Synaptic Vesicles Are Formed In Vitro by Both Clathrin-dependent and Clathrin-independent Pathways.
G. Shi, V. Faundez, J. Roos, E. C. Dell'Angelica, and R. B. Kelly (1998)
J. Cell Biol. 143, 947-955
   Abstract »    Full Text »    PDF »
The Mammalian AP-3 Adaptor-like Complex Mediates the Intracellular Transport of Lysosomal Membrane Glycoproteins.
R. Le Borgne, A. Alconada, U. Bauer, and B. Hoflack (1998)
J. Biol. Chem. 273, 29451-29461
   Abstract »    Full Text »    PDF »
Interaction of Insulin Receptor Substrate-1 with the sigma 3A Subunit of the Adaptor Protein Complex-3 in Cultured Adipocytes.
B. VanRenterghem, M. Morin, M. P. Czech, and R. A. Heller-Harrison (1998)
J. Biol. Chem. 273, 29942-29949
   Abstract »    Full Text »    PDF »
An immunologically distinct beta -adaptin on tubulovesicles of gastric oxyntic cells.
C. T. Okamoto and Y. Y. Jeng (1998)
Am J Physiol Cell Physiol 275, C1323-C1329
   Abstract »    Full Text »    PDF »
An arf1{Delta} Synthetic Lethal Screen Identifies a New Clathrin Heavy Chain Conditional Allele That Perturbs Vacuolar Protein Transport in Saccharomyces cerevisiae.
C.-Y. Chen and T. R. Graham (1998)
Genetics 150, 577-589
   Abstract »    Full Text »
Vesiculation and sorting from PC12-derived endosomes in vitro.
Y. Lichtenstein, C. Desnos, V. Faundez, R. B. Kelly, and L. Clift-O'Grady (1998)
PNAS 95, 11223-11228
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)