Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 20 February 1998:
Vol. 279. no. 5354, pp. 1219 - 1222
DOI: 10.1126/science.279.5354.1219
Prev | Table of Contents | Next

Reports

Identification of a Cullin Homology Region in a Subunit of the Anaphase-Promoting Complex

Hongtao Yu, Jan-Michael Peters, * Randall W. King, Andrew M. Page, Philip Hieter, Marc W. Kirschner dagger

The anaphase-promoting complex is composed of eight protein subunits, including BimE (APC1), CDC27 (APC3), CDC16 (APC6), and CDC23 (APC8). The remaining four human APC subunits, APC2, APC4, APC5, and APC7, as well as human CDC23, were cloned. APC7 contains multiple copies of the tetratrico peptide repeat, similar to CDC16, CDC23, and CDC27. Whereas APC4 and APC5 share no similarity to proteins of known function, APC2 contains a region that is similar to a sequence in cullins, a family of proteins implicated in the ubiquitination of G1 phase cyclins and cyclin-dependent kinase inhibitors. The APC2 gene is essential in Saccharomyces cerevisiae, and apc2 mutants arrest at metaphase and are defective in the degradation of Pds1p. APC2 and cullins may be distantly related members of a ubiquitin ligase family that targets cell cycle regulators for degradation.

H. Yu, J.-M. Peters, R. W. King, M. W. Kirschner, Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA.
A. M. Page and P. Hieter, Centre for Molecular Medicine and Therapeutics, University of British Columbia, Vancouver, BC, V6R2X8, Canada.
*   Present address: Research Institute for Molecular Pathology, Dr. Bohr-Gasse #7, A-1030 Vienna, Austria.

dagger    To whom correspondence should be addressed. E-mail: marc{at}hms.harvard.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Human Cdc34 Employs Distinct Sites To Coordinate Attachment of Ubiquitin to a Substrate and Assembly of Polyubiquitin Chains.
S. Gazdoiu, K. Yamoah, K. Wu, and Z.-Q. Pan (2007)
Mol. Cell. Biol. 27, 7041-7052
   Abstract »    Full Text »    PDF »
Structurally related TPR subunits contribute differently to the function of the anaphase-promoting complex in Drosophila melanogaster.
M. Pal, O. Nagy, D. Menesi, A. Udvardy, and P. Deak (2007)
J. Cell Sci. 120, 3238-3248
   Abstract »    Full Text »    PDF »
Emi1 stably binds and inhibits the anaphase-promoting complex/cyclosome as a pseudosubstrate inhibitor.
J. J. Miller, M. K. Summers, D. V. Hansen, M. V. Nachury, N. L. Lehman, A. Loktev, and P. K. Jackson (2006)
Genes & Dev. 20, 2410-2420
   Abstract »    Full Text »    PDF »
The emerging role of pituitary tumor transforming gene in tumorigenesis..
J. Tfelt-Hansen, D. Kanuparthi, and N. Chattopadhyay (2006)
Clin. Med. Res. 4, 130-137
   Abstract »    Full Text »    PDF »
An architectural map of the anaphase-promoting complex..
B. R. Thornton, T. M. Ng, M. E. Matyskiela, C. W. Carroll, D. O. Morgan, and D. P. Toczyski (2006)
Genes & Dev. 20, 449-460
   Abstract »    Full Text »    PDF »
Mechanistic insight into the allosteric activation of a ubiquitin-conjugating enzyme by RING-type ubiquitin ligases.
E. Ozkan, H. Yu, and J. Deisenhofer (2005)
PNAS 102, 18890-18895
   Abstract »    Full Text »    PDF »
Depletion of Anaphase-promoting Complex or Cyclosome (APC/C) Subunit Homolog APC1 or CDC27 of Trypanosoma brucei Arrests the Procyclic Form in Metaphase but the Bloodstream Form in Anaphase.
P. Kumar and C. C. Wang (2005)
J. Biol. Chem. 280, 31783-31791
   Abstract »    Full Text »    PDF »
In Vivo Characterization of the Nonessential Budding Yeast Anaphase-Promoting Complex/Cyclosome Components Swm1p, Mnd2p and Apc9p.
A. M. Page, V. Aneliunas, J. R. Lamb, and P. Hieter (2005)
Genetics 170, 1045-1062
   Abstract »    Full Text »    PDF »
Arabidopsis Has Two Redundant Cullin3 Proteins That Are Essential for Embryo Development and That Interact with RBX1 and BTB Proteins to Form Multisubunit E3 Ubiquitin Ligase Complexes in Vivo.
P. Figueroa, G. Gusmaroli, G. Serino, J. Habashi, L. Ma, Y. Shen, S. Feng, M. Bostick, J. Callis, H. Hellmann, et al. (2005)
PLANT CELL 17, 1180-1195
   Abstract »    Full Text »    PDF »
BTB Protein Keap1 Targets Antioxidant Transcription Factor Nrf2 for Ubiquitination by the Cullin 3-Roc1 Ligase.
M. Furukawa and Y. Xiong (2005)
Mol. Cell. Biol. 25, 162-171
   Abstract »    Full Text »    PDF »
Changes in the Localization of the Saccharomyces cerevisiae Anaphase-Promoting Complex Upon Microtubule Depolymerization and Spindle Checkpoint Activation.
P. G. Melloy and S. L. Holloway (2004)
Genetics 167, 1079-1094
   Abstract »    Full Text »    PDF »
The Apc5 Subunit of the Anaphase-Promoting Complex/Cyclosome Interacts with Poly(A) Binding Protein and Represses Internal Ribosome Entry Site-Mediated Translation.
N. Koloteva-Levine, D. Pinchasi, I. Pereman, A. Zur, M. Brandeis, and O. Elroy-Stein (2004)
Mol. Cell. Biol. 24, 3577-3587
   Abstract »    Full Text »    PDF »
Suppression of a mitotic mutant by tRNA-Ala anticodon mutations that produce a dominant defect in late mitosis.
Y. Kimata and M. Yanagida (2004)
J. Cell Sci. 117, 2283-2293
   Abstract »    Full Text »    PDF »
Swm1/Apc13 Is an Evolutionarily Conserved Subunit of the Anaphase-Promoting Complex Stabilizing the Association of Cdc16 and Cdc27.
M. Schwickart, J. Havlis, B. Habermann, A. Bogdanova, A. Camasses, T. Oelschlaegel, A. Shevchenko, and W. Zachariae (2004)
Mol. Cell. Biol. 24, 3562-3576
   Abstract »    Full Text »    PDF »
Cullin 3 Promotes Proteasomal Degradation of the Topoisomerase I-DNA Covalent Complex.
H.-F. Zhang, A. Tomida, R. Koshimizu, Y. Ogiso, S. Lei, and T. Tsuruo (2004)
Cancer Res. 64, 1114-1121
   Abstract »    Full Text »    PDF »
Loss of the anaphase-promoting complex in quiescent cells causes unscheduled hepatocyte proliferation.
K. G. Wirth, R. Ricci, J. F. Gimenez-Abian, S. Taghybeeglu, N. R. Kudo, W. Jochum, M. Vasseur-Cognet, and K. Nasmyth (2004)
Genes & Dev. 18, 88-98
   Abstract »    Full Text »    PDF »
VACM-1, a cul-5 gene, inhibits cellular growth by a mechanism that involves MAPK and p53 signaling pathways.
C. Van Dort, P. Zhao, K. Parmelee, B. Capps, A. Poel, L. Listenberger, J. Kossoris, B. Wasilevich, D. Murrey, P. Clare, et al. (2003)
Am J Physiol Cell Physiol 285, C1386-C1396
   Abstract »    Full Text »    PDF »
A Role for Saccharomyces cerevisiae Cul8 Ubiquitin Ligase in Proper Anaphase Progression.
J. J. Michel, J. F. McCarville, and Y. Xiong (2003)
J. Biol. Chem. 278, 22828-22837
   Abstract »    Full Text »    PDF »
Mnd2 and Swm1 Are Core Subunits of the Saccharomyces cerevisiae Anaphase-promoting Complex.
M. C. Hall, M. P. Torres, G. K. Schroeder, and C. H. Borchers (2003)
J. Biol. Chem. 278, 16698-16705
   Abstract »    Full Text »    PDF »
CUL7: A DOC domain-containing cullin selectively binds Skp1{middle dot}Fbx29 to form an SCF-like complex.
D. C. Dias, G. Dolios, R. Wang, and Z.-Q. Pan (2002)
PNAS 99, 16601-16606
   Abstract »    Full Text »    PDF »
Expression analyses and interaction with the anaphase promoting complex protein Apc2 suggest a role for inversin in primary cilia and involvement in the cell cycle.
D. Morgan, L. Eley, J. Sayer, T. Strachan, L. M. Yates, A. S. Craighead, and J. A. Goodship (2002)
Hum. Mol. Genet. 11, 3345-3350
   Abstract »    Full Text »    PDF »
Comparative Genomics of the RBR Family, Including the Parkinson's Disease-Related Gene Parkin and the Genes of the Ariadne Subfamily.
I. Marin and A. Ferrus (2002)
Mol. Biol. Evol. 19, 2039-2050
   Abstract »    Full Text »    PDF »
The Ubiquitin-Dependent Targeting Pathway in Saccharomyces cerevisiae Plays a Critical Role in Multiple Chromatin Assembly Regulatory Steps.
T. A. A. Harkness, G. F. Davies, V. Ramaswamy, and T. G. Arnason (2002)
Genetics 162, 615-632
   Abstract »    Full Text »    PDF »
The anaphase-promoting complex: it's not just for mitosis any more.
J. W. Harper, J. L. Burton, and M. J. Solomon (2002)
Genes & Dev. 16, 2179-2206
   Full Text »    PDF »
The anaphase promoting complex/cyclosome is required during development for modified cell cycles.
H. Kashevsky, J. A. Wallace, B. H. Reed, C. Lai, A. Hayashi-Hagihara, and T. L. Orr-Weaver (2002)
PNAS 99, 11217-11222
   Abstract »    Full Text »    PDF »
Laser Capture Microdissection and Microarray Expression Analysis of Lung Adenocarcinoma Reveals Tobacco Smoking- and Prognosis-related Molecular Profiles.
K. Miura, E. D. Bowman, R. Simon, A. C. Peng, A. I. Robles, R. T. Jones, T. Katagiri, P. He, H. Mizukami, L. Charboneau, et al. (2002)
Cancer Res. 62, 3244-3250
   Abstract »    Full Text »    PDF »
Activation of UBC5 Ubiquitin-conjugating Enzyme by the RING Finger of ROC1 and Assembly of Active Ubiquitin Ligases by All Cullins.
M. Furukawa, T. Ohta, and Y. Xiong (2002)
J. Biol. Chem. 277, 15758-15765
   Abstract »    Full Text »    PDF »
The Ubiquitin-Proteasome Proteolytic Pathway: Destruction for the Sake of Construction.
M. H. Glickman and A. Ciechanover (2002)
Physiol Rev 82, 373-428
   Abstract »    Full Text »    PDF »
CulB, a Putative Ubiquitin Ligase Subunit, Regulates Prestalk Cell Differentiation and Morphogenesis in Dictyostelium spp..
B. Wang and A. Kuspa (2002)
Eukaryot. Cell 1, 126-136
   Abstract »    Full Text »    PDF »
Multiple Subunits of the Caenorhabditis elegans Anaphase-Promoting Complex Are Required for Chromosome Segregation During Meiosis I.
E. S. Davis, L. Wille, B. A. Chestnut, P. L. Sadler, D. C. Shakes, and A. Golden (2002)
Genetics 160, 805-813
   Abstract »    Full Text »    PDF »
Phenotypic characterization of Drosophila ida mutants: defining the role of APC5 in cell cycle progression.
A. M. Bentley, B. C. Williams, M. L. Goldberg, and A. J. Andres (2002)
J. Cell Sci. 115, 949-961
   Abstract »    Full Text »    PDF »
UV-damaged DNA-binding Proteins Are Targets of CUL-4A-mediated Ubiquitination and Degradation.
X. Chen, Y. Zhang, L. Douglas, and P. Zhou (2001)
J. Biol. Chem. 276, 48175-48182
   Abstract »    Full Text »    PDF »
APC2 Cullin Protein and APC11 RING Protein Comprise the Minimal Ubiquitin Ligase Module of the Anaphase-promoting Complex.
Z. Tang, B. Li, R. Bharadwaj, H. Zhu, E. Ozkan, K. Hakala, J. Deisenhofer, and H. Yu (2001)
Mol. Biol. Cell 12, 3839-3851
   Abstract »    Full Text »    PDF »
Substrate recognition by the Cdc20 and Cdh1 components of the anaphase-promoting complex.
C. M. Pfleger, E. Lee, and M. W. Kirschner (2001)
Genes & Dev. 15, 2396-2407
   Abstract »    Full Text »    PDF »
In Vivo Action of the HRD Ubiquitin Ligase Complex: Mechanisms of Endoplasmic Reticulum Quality Control and Sterol Regulation.
R. G. Gardner, A. G. Shearer, and R. Y. Hampton (2001)
Mol. Cell. Biol. 21, 4276-4291
   Abstract »    Full Text »    PDF »
Phosphorylation- and SKP1-independent in Vitro Ubiquitination of E2F1 by Multiple ROC-Cullin Ligases.
T. Ohta and Y. Xiong (2001)
Cancer Res. 61, 1347-1353
   Abstract »    Full Text »
Metaphase to Anaphase (mat) Transition-Defective Mutants inCaenorhabditis elegans.
A. Golden, P. L. Sadler, M. R. Wallenfang, J. M. Schumacher, D. R. Hamill, G. Bates, B. Bowerman, G. Seydoux, and D. C. Shakes (2000)
J. Cell Biol. 151, 1469-1482
   Abstract »    Full Text »    PDF »
The CUL1 C-Terminal Sequence and ROC1 Are Required for Efficient Nuclear Accumulation, NEDD8 Modification, and Ubiquitin Ligase Activity of CUL1.
M. Furukawa, Y. Zhang, J. McCarville, T. Ohta, and Y. Xiong (2000)
Mol. Cell. Biol. 20, 8185-8197
   Abstract »    Full Text »
The RING-H2 finger protein APC11 and the E2 enzyme UBC4 are sufficient to ubiquitinate substrates of the anaphase-promoting complex.
M. Gmachl, C. Gieffers, A. V. Podtelejnikov, M. Mann, and J.-M. Peters (2000)
PNAS 97, 8973-8978
   Abstract »    Full Text »    PDF »
The APC11 RING-H2 Finger Mediates E2-Dependent Ubiquitination.
J. D. Leverson, C. A.P. Joazeiro, A. M. Page, H.-k. Huang, P. Hieter, and T. Hunter (2000)
Mol. Biol. Cell 11, 2315-2325
   Abstract »    Full Text »
Stem-Loop Binding Protein, the Protein That Binds the 3' End of Histone mRNA, Is Cell Cycle Regulated by Both Translational and Posttranslational Mechanisms.
M. L. Whitfield, L.-X. Zheng, A. Baldwin, T. Ohta, M. M. Hurt, and W. F. Marzluff (2000)
Mol. Cell. Biol. 20, 4188-4198
   Abstract »    Full Text »
EMB-30: An APC4 Homologue Required for Metaphase-to-Anaphase Transitions during Meiosis and Mitosis in Caenorhabditis elegans.
T. Furuta, S. Tuck, J. Kirchner, B. Koch, R. Auty, R. Kitagawa, A. M. Rose, and D. Greenstein (2000)
Mol. Biol. Cell 11, 1401-1419
   Abstract »    Full Text »
The SCFHOS/beta -TRCP-ROC1 E3 Ubiquitin Ligase Utilizes Two Distinct Domains within CUL1 for Substrate Targeting and Ubiquitin Ligation.
K. Wu, S. Y. Fuchs, A. Chen, P. Tan, C. Gomez, Z.'e. Ronai, and Z.-Q. Pan (2000)
Mol. Cell. Biol. 20, 1382-1393
   Abstract »    Full Text »
Human Bubr1 Is a Mitotic Checkpoint Kinase That Monitors Cenp-E Functions at Kinetochores and Binds the Cyclosome/APC.
G.K.T. Chan, S.A. Jablonski, V. Sudakin, J.C. Hittle, and T.J. Yen (1999)
J. Cell Biol. 146, 941-954
   Abstract »    Full Text »    PDF »
Components of an SCF ubiquitin ligase localize to the centrosome and regulate the centrosome duplication cycle.
E. Freed, K. R. Lacey, P. Huie, S. A. Lyapina, R. J. Deshaies, T. Stearns, and P. K. Jackson (1999)
Genes & Dev. 13, 2242-2257
   Abstract »    Full Text »
Regulation of APC Activity by Phosphorylation and Regulatory Factors.
S. Kotani, H. Tanaka, H. Yasuda, and K. Todokoro (1999)
J. Cell Biol. 146, 791-800
   Abstract »    Full Text »    PDF »
Studying interactions of four proteins in the yeast two-hybrid system: Structural resemblance of the pVHL/elongin BC/hCUL-2 complex with the ubiquitin ligase complex SKP1/cullin/F-box protein.
A. Pause, B. Peterson, G. Schaffar, R. Stearman, and R. D. Klausner (1999)
PNAS 96, 9533-9538
   Abstract »    Full Text »    PDF »
Whose end is destruction: cell division and the anaphase-promoting complex.
W. Zachariae and K. Nasmyth (1999)
Genes & Dev. 13, 2039-2058
   Full Text »
Pds1p of budding yeast has dual roles: inhibition of anaphase initiation and regulation of mitotic exit.
O. Cohen-Fix and D. Koshland (1999)
Genes & Dev. 13, 1950-1959
   Abstract »    Full Text »
Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34.
J. H. Seol, R.M. R. Feldman, W. Zachariae, A. Shevchenko, C. C. Correll, S. Lyapina, Y. Chi, M. Galova, J. Claypool, S. Sandmeyer, et al. (1999)
Genes & Dev. 13, 1614-1626
   Abstract »    Full Text »
Characterization of the DOC1/APC10 Subunit of the Yeast and the Human Anaphase-promoting Complex.
R. Grossberger, C. Gieffers, W. Zachariae, A. V. Podtelejnikov, A. Schleiffer, K. Nasmyth, M. Mann, and J.-M. Peters (1999)
J. Biol. Chem. 274, 14500-14507
   Abstract »    Full Text »    PDF »
The Schizosaccharomyces pombe dim1+ Gene Interacts with the Anaphase-Promoting Complex or Cyclosome (APC/C) Component lid1+ and Is Required for APC/C Function.
L. D. Berry, A. Feoktistova, M. D. Wright, and K. L. Gould (1999)
Mol. Cell. Biol. 19, 2535-2546
   Abstract »    Full Text »    PDF »
Regulation of Cdc28 Cyclin-Dependent Protein Kinase Activity during the Cell Cycle of the Yeast Saccharomyces cerevisiae.
M. D. Mendenhall and A. E. Hodge (1998)
Microbiol. Mol. Biol. Rev. 62, 1191-1243
   Abstract »    Full Text »    PDF »
A Late Mitotic Regulatory Network Controlling Cyclin Destruction in Saccharomyces cerevisiae.
S. L. Jaspersen, J. F. Charles, R. L. Tinker-Kulberg, and D. O. Morgan (1998)
Mol. Biol. Cell 9, 2803-2817
   Abstract »    Full Text »
Cloning and Expression Analysis of a Novel Salicylate Suppressible Gene, Hs-CUL-3, a Member of Cullin/Cdc53 Family.
M. Du, L. Sansores-Garcia, Z. Zu, and K. K.-y. Wu (1998)
J. Biol. Chem. 273, 24289-24292
   Abstract »    Full Text »    PDF »
Xe-p9, a Xenopus Suc1/Cks protein, is essential for the Cdc2-dependent phosphorylation of the anaphase- promoting complex at mitosis.
D. Patra and W. G. Dunphy (1998)
Genes & Dev. 12, 2549-2559
   Abstract »    Full Text »
The mouse and human genes encoding the recognition component of the N-end rule pathway.
Y. T. Kwon, Y. Reiss, V. A. Fried, A. Hershko, J. K. Yoon, D. K. Gonda, P. Sangan, N. G. Copeland, N. A. Jenkins, and A. Varshavsky (1998)
PNAS 95, 7898-7903
   Abstract »    Full Text »    PDF »
PRT1 of Arabidopsis thaliana encodes a component of the plant N-end rule pathway.
T. Potuschak, S. Stary, P. Schlogelhofer, F. Becker, V. Nejinskaia, and A. Bachmair (1998)
PNAS 95, 7904-7908
   Abstract »    Full Text »    PDF »
Mass Spectrometric Analysis of the Anaphase-Promoting Complex from Yeast: Identification of a Subunit Related to Cullins.
W. Zachariae, A. Shevchenko, P. D. Andrews, R. Ciosk, M. Galova, M. J. Stark, M. Mann, and K. Nasmyth (1998)
Science 279, 1216-1219
   Abstract »    Full Text »
BIMAAPC3, a component of the Aspergillus anaphase promoting complex/cyclosome, is required for a G2 checkpoint blocking entry into mitosis in the absence of NIMA function.
C. Lies, J Cheng, S. James, N. Morris, M. O'Connell, and P. Mirabito (1998)
J. Cell Sci. 111, 1453-1465
   Abstract »    PDF »
Membrane Topology and Function of Der3/Hrd1p as a Ubiquitin-Protein Ligase (E3) Involved in Endoplasmic Reticulum Degradation.
P. M. Deak and D. H. Wolf (2001)
J. Biol. Chem. 276, 10663-10669
   Abstract »    Full Text »    PDF »
The evolutionarily conserved Dim1 protein defines a novel branch of the thioredoxin fold superfamily.
Y.-Z. ZHANG, K. L. GOULD, R. L. DUNBRACK JR., H. CHENG, H. RODER, and E. A. GOLEMIS (1999)
Physiol Genomics 1, 109-118
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)