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Science 20 February 1998:
Vol. 279. no. 5354, pp. 1190 - 1193
DOI: 10.1126/science.279.5354.1190
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Reports

Probing Single Secretory Vesicles with Capillary Electrophoresis

Daniel T. Chiu, Sheri J. Lillard, Richard H. Scheller, Richard N. Zare, * Sandra E. Rodriguez-Cruz, Evan R. Williams, Owe Orwar, Mats Sandberg, J. Anders Lundqvist

Secretory vesicles obtained from the atrial gland of the gastropod mollusk Aplysia californica were chemically analyzed individually with a combination of optical trapping, capillary electrophoresis separation, and a laser-induced fluorescence detection. With the use of optical trapping, a single vesicle that had attoliters (10-18 liters) of volume was introduced into the tapered inlet of a separation capillary. Once the vesicle was injected, it was lysed, and its components were fluorescently labeled with naphthalene-2,3-dicarboxaldehyde before separation. The resultant electropherograms indicated distinct variations in the contents of single vesicles.

D. T. Chiu, S. J. Lillard, R. N. Zare, Department of Chemistry, Stanford University, Stanford, CA 94305, USA.
R. H. Scheller, Department of Molecular and Cellular Physiology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
S. E. Rodriguez-Cruz and E. R. Williams, Department of Chemistry, University of California, Berkeley, CA 94720, USA.
O. Orwar and J. A. Lundqvist, Department of Chemistry, Göteborg University, SE-412 96, Göteborg, Sweden.
M. Sandberg, Department of Anatomy and Cell Biology, Göteborg University, SE-413 90, Göteborg, Sweden.
*   To whom correspondence should be addressed.

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