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Science 2 January 1998:
Vol. 279. no. 5347, pp. 95 - 98
DOI: 10.1126/science.279.5347.95
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Reports

Discrete Start Sites for DNA Synthesis in the Yeast ARS1 Origin

Anja-Katrin Bielinsky, Susan A. Gerbi *

Sites of DNA synthesis initiation have been detected at the nucleotide level in a yeast origin of bidirectional replication with the use of replication initiation point mapping. The ARS1 origin of Saccharomyces cerevisiae showed a transition from discontinuous to continuous DNA synthesis in an 18-base pair region (nucleotides 828 to 845) from within element B1 toward B2, adjacent to the binding site for the origin recognition complex, the putative initiator protein.

Department of Molecular Biology, Cell Biology and Biochemistry, Division of Biology and Medicine, Brown University, Providence, RI 02912, USA.
*   To whom correspondence should be addressed. E-mail: susan gerbi{at}brown.edu

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