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Science 17 October 1997:
Vol. 278. no. 5337, pp. 460 - 463
DOI: 10.1126/science.278.5337.460
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Reports

CDC20 and CDH1: A Family of Substrate-Specific Activators of APC-Dependent Proteolysis

Rosella Visintin, Susanne Prinz, Angelika Amon *

Proteolysis mediated by the anaphase-promoting complex (APC) triggers chromosome segregation and exit from mitosis, yet its regulation is poorly understood. The conserved Cdc20 and Cdh1 proteins were identified as limiting, substrate-specific activators of APC-dependent proteolysis. CDC20 was required for the degradation of the APC substrate Pds1 but not for that of other APC substrates, such as Clb2 and Ase1. Conversely, cdh1Delta mutants were impaired in the degradation of Ase1 and Clb2 but not in that of Pds1. Overexpression of either CDC20 or CDH1 was sufficient to induce APC-dependent proteolysis of the appropriate target in stages of the cell cycle in which substrates are normally stable.

Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA.
*   To whom correspondence should be addressed.

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Control of localization of a spindle checkpoint protein, Mad2, in fission yeast.
A. E. Ikui, K. Furuya, M. Yanagida, and T. Matsumoto (2002)
J. Cell Sci. 115, 1603-1610
   Abstract »    Full Text »    PDF »
Inhibition of APC-mediated proteolysis by the meiosis-specific protein kinase Ime2.
M. Bolte, P. Steigemann, G. H. Braus, and S. Irniger (2002)
PNAS 99, 4385-4390
   Abstract »    Full Text »    PDF »
Transcriptional silencing in Saccharomyces cerevisiae and Schizosaccharomyces pombe.
Y. Huang (2002)
Nucleic Acids Res. 30, 1465-1482
   Abstract »    Full Text »    PDF »
The Ubiquitin-Proteasome Proteolytic Pathway: Destruction for the Sake of Construction.
M. H. Glickman and A. Ciechanover (2002)
Physiol Rev 82, 373-428
   Abstract »    Full Text »    PDF »
Evidence That the pre-mRNA Splicing Factor Clf1p Plays a Role in DNA Replication in Saccharomyces cerevisiae.
W. Zhu, I. R. Rainville, M. Ding, M. Bolus, N. H. Heintz, and D. S. Pederson (2002)
Genetics 160, 1319-1333
   Abstract »    Full Text »    PDF »
Checkpoint Protein BubR1 Acts Synergistically with Mad2 to Inhibit Anaphase-promoting Complex.
G. Fang (2002)
Mol. Biol. Cell 13, 755-766
   Abstract »    Full Text »    PDF »
fzr-1 and lin-35/Rb function redundantly to control cell proliferation in C. elegans as revealed by a nonbiased synthetic screen.
D. S. Fay, S. Keenan, and M. Han (2002)
Genes & Dev. 16, 503-517
   Abstract »    Full Text »    PDF »


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