Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 12 September 1997:
Vol. 277. no. 5332, pp. 1662 - 1666
DOI: 10.1126/science.277.5332.1662
Prev | Table of Contents | Next

Reports

Structure of a Murine Leukemia Virus Receptor-Binding Glycoprotein at 2.0 Angstrom Resolution

Deborah Fass, Robert A. Davey, Christian A. Hamson, Peter S. Kim, * James M. Cunningham, * James M. Berger

An essential step in retrovirus infection is the binding of the virus to its receptor on a target cell. The structure of the receptor-binding domain of the envelope glycoprotein from Friend murine leukemia virus was determined to 2.0 angstrom resolution by x-ray crystallography. The core of the domain is an antiparallel beta  sandwich, with two interstrand loops forming a helical subdomain atop the sandwich. The residues in the helical region, but not in the beta  sandwich, are highly variable among mammalian C-type retroviruses with distinct tropisms, indicating that the helical subdomain determines the receptor specificity of the virus.

D. Fass and P. S. Kim, Howard Hughes Medical Institute, Whitehead Institute for Biomedical Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA.
R. A. Davey, C. A. Hamson, J. M. Cunningham, Howard Hughes Medical Institute, Division of Hematology, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Room 30, Thorn Building, 20 Shattuck Street, Boston, MA 02115, USA.
J. M. Berger, Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.
*   To whom correspondence should be addressed.

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Engineering the Surface Glycoproteins of Lentiviral Vectors for Targeted Gene Transfer.
E. Verhoeyen and F.-L. Cosset (2009)
CSH Protocols 2009, pdb.top59
   Abstract »    Full Text »
The Degree of Folding Instability of the Envelope Protein of a Neurovirulent Murine Retrovirus Correlates with the Severity of the Neurological Disease.
J. L. Portis, P. Askovich, J. Austin, Y. Gutierrez-Cotto, and F. J. McAtee (2009)
J. Virol. 83, 6079-6086
   Abstract »    Full Text »    PDF »
Neurovirulence of Polytropic Murine Retrovirus Is Influenced by Two Separate Regions on Opposite Sides of the Envelope Protein Receptor Binding Domain.
K. E. Peterson, S. Pourciau, M. Du, R. LaCasse, M. Pathmajeyan, D. Poulsen, M. Agbandje-McKenna, K. Wehrly, and B. Chesebro (2008)
J. Virol. 82, 8906-8910
   Abstract »    Full Text »    PDF »
Binding of equine infectious anemia virus to the equine lentivirus receptor-1 is mediated by complex discontinuous sequences in the viral envelope gp90 protein.
C. Sun, B. Zhang, J. Jin, and R. C. Montelaro (2008)
J. Gen. Virol. 89, 2011-2019
   Abstract »    Full Text »    PDF »
Cell Surface Expression of the Bovine Leukemia Virus-Binding Receptor on B and T Lymphocytes Is Induced by Receptor Engagement.
M. Lavanya, S. Kinet, A. Montel-Hagen, C. Mongellaz, J.-L. Battini, M. Sitbon, and N. Taylor (2008)
J. Immunol. 181, 891-898
   Abstract »    Full Text »    PDF »
Changes in viral protein function that accompany retroviral endogenization.
N. M. Oliveira, H. Satija, I. A. Kouwenhoven, and M. V. Eiden (2007)
PNAS 104, 17506-17511
   Abstract »    Full Text »    PDF »
TR1.3 Viral Pathogenesis and Syncytium Formation Are Linked to Env-Gag Cooperation.
S. L. Murphy and G. N. Gaulton (2007)
J. Virol. 81, 10777-10785
   Abstract »    Full Text »    PDF »
Identification of a retroviral receptor used by an Envelope protein derived by peptide library screening.
A. Sarangi, K. Bupp, and M. J. Roth (2007)
PNAS 104, 11032-11037
   Abstract »    Full Text »    PDF »
From the Cover: An infectious retrovirus susceptible to an IFN antiviral pathway from human prostate tumors.
B. Dong, S. Kim, S. Hong, J. Das Gupta, K. Malathi, E. A. Klein, D. Ganem, J. L. DeRisi, S. A. Chow, and R. H. Silverman (2007)
PNAS 104, 1655-1660
   Abstract »    Full Text »    PDF »
Conserved Receptor-binding Domains of Lake Victoria Marburgvirus and Zaire Ebolavirus Bind a Common Receptor.
J. H. Kuhn, S. R. Radoshitzky, A. C. Guth, K. L. Warfield, W. Li, M. J. Vincent, J. S. Towner, S. T. Nichol, S. Bavari, H. Choe, et al. (2006)
J. Biol. Chem. 281, 15951-15958
   Abstract »    Full Text »    PDF »
Linkage of Reduced Receptor Affinity and Superinfection to Pathogenesis of TR1.3 Murine Leukemia Virus.
S. L. Murphy, M. Chung-Landers, M. Honczarenko, and G. N. Gaulton (2006)
J. Virol. 80, 4601-4609
   Abstract »    Full Text »    PDF »
Involvement of the C-Terminal Disulfide-Bonded Loop of Murine Leukemia Virus SU Protein in a Postbinding Step Critical for Viral Entry.
M. D. Burkhart, P. D'Agostino, S. C. Kayman, and A. Pinter (2005)
J. Virol. 79, 7868-7876
   Abstract »    Full Text »    PDF »
Retrovirus envelope protein complex structure in situ studied by cryo-electron tomography.
F. Forster, O. Medalia, N. Zauberman, W. Baumeister, and D. Fass (2005)
PNAS 102, 4729-4734
   Abstract »    Full Text »    PDF »
Novel Host Range and Cytopathic Variant of Ecotropic Friend Murine Leukemia Virus.
Y. T. Jung, T. Wu, and C. A. Kozak (2004)
J. Virol. 78, 12189-12197
   Abstract »    Full Text »    PDF »
Change of Tropism of SL3-2 Murine Leukemia Virus, Using Random Mutational Libraries.
S. Bahrami, M. Duch, and F. S. Pedersen (2004)
J. Virol. 78, 9343-9351
   Abstract »    Full Text »    PDF »
Complementation of a Binding-Defective Retrovirus by a Host Cell Receptor Mutant.
Z. Qian, H. Wang, C. Empig, W. F. Anderson, and L. M. Albritton (2004)
J. Virol. 78, 5766-5772
   Abstract »    Full Text »    PDF »
Identification of the Receptor Binding Domain of the Mouse Mammary Tumor Virus Envelope Protein.
Y. Zhang, J. C. Rassa, M. E. deObaldia, L. M. Albritton, and S. R. Ross (2003)
J. Virol. 77, 10468-10478
   Abstract »    Full Text »    PDF »
Generation of Novel Syncytium-Inducing and Host Range Variants of Ecotropic Moloney Murine Leukemia Virus in Mus spicilegus.
Y. T. Jung and C. A. Kozak (2003)
J. Virol. 77, 5065-5072
   Abstract »    Full Text »    PDF »
Distinct Mechanisms of Neutralization by Monoclonal Antibodies Specific for Sites in the N-Terminal or C-Terminal Domain of Murine Leukemia Virus SU.
M. D. Burkhart, S. C. Kayman, Y. He, and A. Pinter (2003)
J. Virol. 77, 3993-4003
   Abstract »    Full Text »    PDF »
A Virus-Virus Interaction Circumvents the Virus Receptor Requirement for Infection by Pathogenic Retroviruses.
D. L. Wensel, W. Li, and J. M. Cunningham (2003)
J. Virol. 77, 3460-3469
   Abstract »    Full Text »    PDF »
Structure and Mechanism of a Coreceptor for Infection by a Pathogenic Feline Retrovirus.
A. L. Barnett, D. L. Wensel, W. Li, D. Fass, and J. M. Cunningham (2003)
J. Virol. 77, 2717-2729
   Abstract »    Full Text »    PDF »
Envelope Proteins Containing Single Amino Acid Substitutions Support a Structural Model of the Receptor-Binding Domain of Bovine Leukemia Virus Surface Protein.
E. R. Johnston, L. M. Albritton, and K. Radke (2002)
J. Virol. 76, 10861-10872
   Abstract »    Full Text »    PDF »
Relationship between SU Subdomains That Regulate the Receptor-Mediated Transition from the Native (Fusion-Inhibited) to the Fusion-Active Conformation of the Murine Leukemia Virus Glycoprotein.
D. Lavillette, A. Ruggieri, B. Boson, M. Maurice, and F.-L. Cosset (2002)
J. Virol. 76, 9673-9685
   Abstract »    Full Text »    PDF »
Bovine Leukemia Virus SU Protein Interacts with Zinc, and Mutations within Two Interacting Regions Differently Affect Viral Fusion and Infectivity In Vivo.
J.-S. Gatot, I. Callebaut, C. Van Lint, D. Demonte, P. Kerkhofs, D. Portetelle, A. Burny, L. Willems, and R. Kettmann (2002)
J. Virol. 76, 7956-7967
   Abstract »    Full Text »    PDF »
Genetic and Biochemical Analyses of Receptor and Cofactor Determinants for T-Cell-Tropic Feline Leukemia Virus Infection.
A. S. Lauring, H. H. Cheng, M. V. Eiden, and J. Overbaugh (2002)
J. Virol. 76, 8069-8078
   Abstract »    Full Text »    PDF »
Quaternary Structure of Coronavirus Spikes in Complex with Carcinoembryonic Antigen-related Cell Adhesion Molecule Cellular Receptors.
D. N. Lewicki and T. M. Gallagher (2002)
J. Biol. Chem. 277, 19727-19734
   Abstract »    Full Text »    PDF »
A Unique Heparin-Binding Domain in the Envelope Protein of the Neuropathogenic PVC-211 Murine Leukemia Virus May Contribute to Its Brain Capillary Endothelial Cell Tropism.
A. Jinno-Oue, M. Oue, and S. K. Ruscetti (2001)
J. Virol. 75, 12439-12445
   Abstract »    Full Text »    PDF »
Comprehensive Mutational Analysis of the Moloney Murine Leukemia Virus Envelope Protein.
S. M. Rothenberg, M. N. Olsen, L. C. Laurent, R. A. Crowley, and P. O. Brown (2001)
J. Virol. 75, 11851-11862
   Abstract »    Full Text »
Two Point Mutations Produce Infectious Retrovirus Bearing a Green Fluorescent Protein-SU Fusion Protein.
K. Kizhatil, A. Gromley, and L. M. Albritton (2001)
J. Virol. 75, 11881-11885
   Abstract »    Full Text »
Fv-4: Identification of the Defect in Env and the Mechanism of Resistance to Ecotropic Murine Leukemia Virus.
G. M. Taylor, Y. Gao, and D. A. Sanders (2001)
J. Virol. 75, 11244-11248
   Abstract »    Full Text »    PDF »
Receptor Binding Transforms the Surface Subunit of the Mammalian C-Type Retrovirus Envelope Protein from an Inhibitor to an Activator of Fusion.
A. L. Barnett and J. M. Cunningham (2001)
J. Virol. 75, 9096-9105
   Abstract »    Full Text »    PDF »
Receptors and Entry Cofactors for Retroviruses Include Single and Multiple Transmembrane-Spanning Proteins as well as Newly Described Glycophosphatidylinositol-Anchored and Secreted Proteins.
J. Overbaugh, A. D. Miller, and M. V. Eiden (2001)
Microbiol. Mol. Biol. Rev. 65, 371-389
   Abstract »    Full Text »    PDF »
Identification of Envelope Determinants of Feline Leukemia Virus Subgroup B That Permit Infection and Gene Transfer to Cells Expressing Human Pit1 or Pit2.
J. Sugai, M. Eiden, M. M. Anderson, N. Van Hoeven, C. D. Meiering, and J. Overbaugh (2001)
J. Virol. 75, 6841-6849
   Abstract »    Full Text »
Adaptation of Chimeric Retroviruses In Vitro and In Vivo: Isolation of Avian Retroviral Vectors with Extended Host Range.
E. V. Barsov, W. S. Payne, and S. H. Hughes (2001)
J. Virol. 75, 4973-4983
   Abstract »    Full Text »
Mus cervicolor Murine Leukemia Virus Isolate M813 Belongs to a Unique Receptor Interference Group.
V. Prassolov, S. Hein, M. Ziegler, D. Ivanov, C. Münk, J. Löhler, and C. Stocking (2001)
J. Virol. 75, 4490-4498
   Abstract »    Full Text »
Activation of Membrane Fusion by Murine Leukemia Viruses Is Controlled in cis or in trans by Interactions between the Receptor-Binding Domain and a Conserved Disulfide Loop of the Carboxy Terminus of the Surface Glycoprotein.
D. Lavillette, B. Boson, S. J. Russell, and F.-L. Cosset (2001)
J. Virol. 75, 3685-3695
   Abstract »    Full Text »
Modular organization of the Friend murine leukemia virus envelope protein underlies the mechanism of infection.
A. L. Barnett, R. A. Davey, and J. M. Cunningham (2001)
PNAS 98, 4113-4118
   Abstract »    Full Text »    PDF »
Feline Leukemia Virus Envelope Sequences That Affect T-Cell Tropism and Syncytium Formation Are Not Part of Known Receptor-Binding Domains.
S. R. Gwynn, F. C. Hankenson, A. S. Lauring, J. L. Rohn, and J. Overbaugh (2000)
J. Virol. 74, 5754-5761
   Abstract »    Full Text »
Oligomeric Modeling and Electrostatic Analysis of the gp120 Envelope Glycoprotein of Human Immunodeficiency Virus.
P. D. Kwong, R. Wyatt, Q. J. Sattentau, J. Sodroski, and W. A. Hendrickson (2000)
J. Virol. 74, 1961-1972
   Abstract »    Full Text »
A Comprehensive Approach to Mapping the Interacting Surfaces of Murine Amphotropic and Feline Subgroup B Leukemia Viruses with Their Cell Surface Receptors.
C. S. Tailor, A. Nouri, and D. Kabat (2000)
J. Virol. 74, 237-244
   Abstract »    Full Text »
Activation of a Cell Entry Pathway Common to Type C Mammalian Retroviruses by Soluble Envelope Fragments.
D. Lavillette, A. Ruggieri, S. J. Russell, and F.-L. Cosset (2000)
J. Virol. 74, 295-304
   Abstract »    Full Text »
Brain Infection by Neuroinvasive but Avirulent Murine Oncornaviruses.
S. Askovic, F. J. McAtee, C. Favara, and J. L. Portis (2000)
J. Virol. 74, 465-473
   Abstract »    Full Text »
Second-Site Changes Affect Viability of Amphotropic/Ecotropic Chimeric Enveloped Murine Leukemia Viruses.
L. O'Reilly and M. J. Roth (2000)
J. Virol. 74, 899-913
   Abstract »    Full Text »
A Hydrophobic Patch in Ecotropic Murine Leukemia Virus Envelope Protein Is the Putative Binding Site for a Critical Tyrosine Residue on the Cellular Receptor.
T. Zavorotinskaya and L. M. Albritton (1999)
J. Virol. 73, 10164-10172
   Abstract »    Full Text »
Induction of Syncytia by Neuropathogenic Murine Leukemia Viruses Depends on Receptor Density, Host Cell Determinants, and the Intrinsic Fusion Potential of Envelope Protein.
M. Chung, K. Kizhatil, L. M. Albritton, and G. N. Gaulton (1999)
J. Virol. 73, 9377-9385
   Abstract »    Full Text »    PDF »
Failure To Cleave Murine Leukemia Virus Envelope Protein Does Not Preclude Its Incorporation in Virions and Productive Virus-Receptor Interaction.
T. Zavorotinskaya and L. M. Albritton (1999)
J. Virol. 73, 5621-5629
   Abstract »    Full Text »
Suppression of a Fusion Defect by Second Site Mutations in the Ecotropic Murine Leukemia Virus Surface Protein.
T. Zavorotinskaya and L. M. Albritton (1999)
J. Virol. 73, 5034-5042
   Abstract »    Full Text »
Identification of a Receptor-Binding Pocket on the Envelope Protein of Friend Murine Leukemia Virus.
R. A. Davey, Y. Zuo, and J. M. Cunningham (1999)
J. Virol. 73, 3758-3763
   Abstract »    Full Text »
Identification of the block in targeted retroviral-mediated gene transfer.
Y. Zhao, L. Zhu, S. Lee, L. Li, E. Chang, N.-W. Soong, D. Douer, and W. F. Anderson (1999)
PNAS 96, 4005-4010
   Abstract »    Full Text »    PDF »
The Hypervariable Domain of the Murine Leukemia Virus Surface Protein Tolerates Large Insertions and Deletions, Enabling Development of a Retroviral Particle Display System.
S. C. Kayman, H. Park, M. Saxon, and A. Pinter (1999)
J. Virol. 73, 1802-1808
   Abstract »    Full Text »
A human cell-surface receptor for xenotropic and polytropic murine leukemia viruses: Possible role in G protein-coupled signal transduction.
J.-L. Battini, J. E. J. Rasko, and A. D. Miller (1999)
PNAS 96, 1385-1390
   Abstract »    Full Text »    PDF »
A Proline-Rich Motif Downstream of the Receptor Binding Domain Modulates Conformation and Fusogenicity of Murine Retroviral Envelopes.
D. Lavillette, M. Maurice, C. Roche, S. J. Russell, M. Sitbon, and F.-L. Cosset (1998)
J. Virol. 72, 9955-9965
   Abstract »    Full Text »    PDF »
Role of Variable Regions A and B in Receptor Binding Domain of Amphotropic Murine Leukemia Virus Envelope Protein.
J.-Y. Han, Y. Zhao, W. F. Anderson, and P. M. Cannon (1998)
J. Virol. 72, 9101-9108
   Abstract »    Full Text »    PDF »
Sequence Analysis of Mus dunni Endogenous Virus Reveals a Hybrid VL30/Gibbon Ape Leukemia Virus-Like Structure and a Distinct Envelope.
G. Wolgamot, L. Bonham, and A. D. Miller (1998)
J. Virol. 72, 7459-7466
   Abstract »    Full Text »    PDF »
Moloney Murine Leukemia Virus Envelope Protein Subunits, gp70 and Pr15E, Form a Stable Disulfide-Linked Complex.
D.-J. E. Opstelten, M. Wallin, and H. Garoff (1998)
J. Virol. 72, 6537-6545
   Abstract »    Full Text »    PDF »
Supramolecular organization of immature and mature murine leukemia virus revealed by electron cryo-microscopy: Implications for retroviral assembly mechanisms.
M. Yeager, E. M. Wilson-Kubalek, S. G. Weiner, P. O. Brown, and A. Rein (1998)
PNAS 95, 7299-7304
   Abstract »    Full Text »    PDF »
Single Amino Acid Insertion in Loop 4 Confers Amphotropic Murine Leukemia Virus Receptor Function upon Murine Pit1.
M. D. Lundorf, F. S. Pedersen, B. O'Hara, and L. Pedersen (1998)
J. Virol. 72, 4524-4527
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)