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Science 29 August 1997:
Vol. 277. no. 5330, pp. 1313 - 1316
DOI: 10.1126/science.277.5330.1313
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Reports

Accelerated Aging and Nucleolar Fragmentation in Yeast sgs1 Mutants

David A. Sinclair, Kevin Mills, Leonard Guarente

The SGS1 gene of yeast encodes a DNA helicase with homology to the human WRN gene. Mutations in WRN result in Werner's syndrome, a disease with symptoms resembling premature aging. Mutation of SGS1 is shown to cause premature aging in yeast mother cells on the basis of a shortened life-span and the aging-induced phenotypes of sterility and redistribution of the Sir3 silencing protein from telomeres to the nucleolus. Further, in old sgs1 cells the nucleolus is enlarged and fragmented--changes that also occur in old wild-type cells. These findings suggest a conserved mechanism of cellular aging that may be related to nucleolar structure.

Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Volume 277, Number 5330, Issue of 29 August 1997, pp. 1313-1316
©1997 by The American Association for the Advancement of Science.

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