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Science 11 July 1997:
Vol. 277. no. 5323, pp. 235 - 239
DOI: 10.1126/science.277.5323.235
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Reports

Abnormal Lignin in a Loblolly Pine Mutant

John Ralph, * John J. MacKay, Ronald D. Hatfield, David M. O'Malley, Ross W. Whetten, Ronald R. Sederoff

Novel lignin is formed in a mutant loblolly pine (Pinus taeda L.) severely depleted in cinnamyl alcohol dehydrogenase (E.C. 1.1.1.195), which converts coniferaldehyde to coniferyl alcohol, the primary lignin precursor in pines. Dihydroconiferyl alcohol, a monomer not normally associated with the lignin biosynthetic pathway, is the major component of the mutant's lignin, accounting for ~30 percent (versus ~3 percent in normal pine) of the units. The level of aldehydes, including new 2-methoxybenzaldehydes, is also increased. The mutant pines grew normally indicating that, even within a species, extensive variations in lignin composition need not disrupt the essential functions of lignin.

J. Ralph, U.S. Dairy Forage Research Center, U.S. Department of Agriculture (USDA)-Agricultural Research Service (ARS), Madison, WI 53706-1108, and Department of Forestry, University of Wisconsin, Madison, WI 53706-1598, USA.
J. J. MacKay, Department of Genetics, North Carolina State University (NCSU), Raleigh, NC 27695-7614, USA.
R. D. Hatfield, U.S. Dairy Forage Research Center, USDA-ARS, Madison, WI 53706-1108, USA.
D. M. O'Malley and R. W. Whetten, Department of Forestry, NCSU, Raleigh, NC 27695-8008, USA.
R. R. Sederoff, Department of Genetics, NCSU, Raleigh, NC 27695-7614 and Department of Forestry, NCSU, Raleigh, NC 27695-8008, USA.
*   To whom correspondence should be addressed at U.S. Dairy Forage Research Center, USDA-ARS, 1925 Linden Drive West, Madison, WI 53706-1108, USA. E-mail: jralph{at}facstaff.wisc.edu

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