Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.


Science 6 June 1997:
Vol. 276. no. 5318, pp. 1566 - 1568
DOI: 10.1126/science.276.5318.1566

Reports

Aluminum Tolerance in Transgenic Plants by Alteration of Citrate Synthesis

Juan Manuel de la Fuente, * Verenice Ramírez-Rodríguez, * José Luis Cabrera-Ponce, Luis Herrera-Estrella dagger

Aluminum when in soluble form, as found in acidic soils that comprise about 40 percent of the world's arable land, is toxic to many crops. Organic acid excretion has been correlated with aluminum tolerance in higher plants. Overproduction of citrate was shown to result in aluminum tolerance in transgenic tobacco (Nicotiana tabacum) and papaya (Carica papaya) plants.

Departamento de Ingeniería Genética, Centro de Investigacion y Estudios Avanzados, Unidad Irapuato, Apdo, Postal 629 (36500) Irapuato, Guanajuato, México.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed at Departamento de Ingeniería Genética, Centro de Investigaciones y Estudios Avanzados (CINVESTAV), Unidad Irapuato, Apdo, Postal 629 (36500) Irapuato, Gto, México. E-mail: lherrera{at}irapuato.ira.cinvestav.mx


Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Decreased Mitochondrial Activities of Malate Dehydrogenase and Fumarase in Tomato Lead to Altered Root Growth and Architecture via Diverse Mechanisms.
M. J. van der Merwe, S. Osorio, T. Moritz, A. Nunes-Nesi, and A. R. Fernie (2009)
Plant Physiology 149, 653-669
   Abstract »    Full Text »    PDF »
Overexpression of an auxilin-like gene (F9E10.5) can suppress Al uptake in roots of Arabidopsis.
B. Ezaki, H. Kiyohara, H. Matsumoto, and S. Nakashima (2007)
J. Exp. Bot. 58, 497-506
   Abstract »    Full Text »    PDF »
The BnALMT1 and BnALMT2 Genes from Rape Encode Aluminum-Activated Malate Transporters That Enhance the Aluminum Resistance of Plant Cells.
A. Ligaba, M. Katsuhara, P. R. Ryan, M. Shibasaka, and H. Matsumoto (2006)
Plant Physiology 142, 1294-1303
   Abstract »    Full Text »    PDF »
Citrate Transporters Play a Critical Role in Aluminium-stimulated Citrate Efflux in Rice Bean (Vigna umbellata) Roots.
J. L. YANG, L. ZHANG, Y. Y. LI, J. F. YOU, P. WU, and S. J. ZHENG (2006)
Ann. Bot. 97, 579-584
   Abstract »    Full Text »    PDF »
Functions of two genes in aluminium (Al) stress resistance: repression of oxidative damage by the AtBCB gene and promotion of efflux of Al ions by the NtGDI1gene.
B. Ezaki, K. Sasaki, H. Matsumoto, and S. Nakashima (2005)
J. Exp. Bot. 56, 2661-2671
   Abstract »    Full Text »    PDF »
Engineering high-level aluminum tolerance in barley with the ALMT1 gene.
E. Delhaize, P. R. Ryan, D. M. Hebb, Y. Yamamoto, T. Sasaki, and H. Matsumoto (2004)
PNAS 101, 15249-15254
   Abstract »    Full Text »    PDF »
Mechanism of Gene Expression of Arabidopsis Glutathione S-Transferase, AtGST1, and AtGST11 in Response to Aluminum Stress.
B. Ezaki, M. Suzuki, H. Motoda, M. Kawamura, S. Nakashima, and H. Matsumoto (2004)
Plant Physiology 134, 1672-1682
   Abstract »    Full Text »    PDF »
Overexpression of ATP Sulfurylase in Indian Mustard: Effects on Tolerance and Accumulation of Twelve Metals.
A. L. Wangeline, J. L. Burkhead, K. L. Hale, S. D. Lindblom, N. Terry, M. Pilon, and E. A. H. Pilon-Smits (2004)
J. Environ. Qual. 33, 54-60
   Abstract »    Full Text »    PDF »
Comparison of Al-induced gene expression in sensitive and tolerant soybean cultivars.
V. Ermolayev, W. Weschke, and R. Manteuffel (2003)
J. Exp. Bot. 54, 2745-2756
   Abstract »    Full Text »    PDF »
Alteration of Citrate Metabolism in Cluster Roots of White Lupin.
T. Kihara, T. Wada, Y. Suzuki, T. Hara, and H. Koyama (2003)
Plant Cell Physiol. 44, 901-908
   Abstract »    Full Text »    PDF »
Modulation of Citrate Metabolism Alters Aluminum Tolerance in Yeast and Transgenic Canola Overexpressing a Mitochondrial Citrate Synthase.
V. M. Anoop, U. Basu, M. T. McCammon, L. McAlister-Henn, and G. J. Taylor (2003)
Plant Physiology 132, 2205-2217
   Abstract »    Full Text »    PDF »
Al-induced efflux of organic acid anions is poorly associated with internal organic acid metabolism in triticale roots.
J. E. Hayes and J. F. Ma (2003)
J. Exp. Bot. 54, 1753-1759
   Abstract »    Full Text »    PDF »
Analysis of Transgenic Indian Mustard Plants for Phytoremediation of Metal-Contaminated Mine Tailings.
L. E. Bennett, J. L. Burkhead, K. L. Hale, N. Terry, M. Pilon, and E. A. H. Pilon-Smits (2003)
J. Environ. Qual. 32, 432-440
   Abstract »    Full Text »    PDF »
Characterization of Citrate Transport through the Plasma Membrane in a Carrot Mutant Cell Line with Enhanced Citrate Excretion.
T. Ohno, H. Koyama, and T. Hara (2003)
Plant Cell Physiol. 44, 156-162
   Abstract »    Full Text »    PDF »
QTL Analysis of Al Tolerance in Recombinant Inbred Lines of Arabidopsis thaliana.
Y. Kobayashi and H. Koyama (2002)
Plant Cell Physiol. 43, 1526-1533
   Abstract »    Full Text »    PDF »
Food Biotechnology: Benefits and Concerns.
M. C. Falk, B. M. Chassy, S. K. Harlander, T. J. Hoban IV, M. N. McGloughlin, and A. R. Akhlaghi (2002)
J. Nutr. 132, 1384-1390
   Abstract »    Full Text »    PDF »
A Gene Encoding Multidrug Resistance (MDR)-Like Protein is Induced by Aluminum and Inhibitors of Calcium Flux in Wheat.
T. Sasaki, B. Ezaki, and H. Matsumoto (2002)
Plant Cell Physiol. 43, 177-185
   Abstract »    Full Text »    PDF »
Overexpression of Malate Dehydrogenase in Transgenic Alfalfa Enhances Organic Acid Synthesis and Confers Tolerance to Aluminum.
M. Tesfaye, S. J. Temple, D. L. Allan, C. P. Vance, and D. A. Samac (2001)
Plant Physiology 127, 1836-1844
   Abstract »    Full Text »    PDF »
Different Mechanisms of Four Aluminum (Al)-Resistant Transgenes for Al Toxicity in Arabidopsis.
B. Ezaki, M. Katsuhara, M. Kawamura, and H. Matsumoto (2001)
Plant Physiology 127, 918-927
   Abstract »    Full Text »    PDF »
Possible Involvement of Protein Phosphorylation in Aluminum-Responsive Malate Efflux from Wheat Root Apex.
H. Osawa and H. Matsumoto (2001)
Plant Physiology 126, 411-420
   Abstract »    Full Text »
Expression of a Pseudomonas aeruginosa Citrate Synthase Gene in Tobacco Is Not Associated with Either Enhanced Citrate Accumulation or Efflux.
E. Delhaize, D. M. Hebb, and P. R. Ryan (2001)
Plant Physiology 125, 2059-2067
   Abstract »    Full Text »
The High Level of Aluminum Resistance in Signalgrass Is Not Associated with Known Mechanisms of External Aluminum Detoxification in Root Apices.
P. Wenzl, G. M. Patiño, A. L. Chaves, J. E. Mayer, and I. M. Rao (2001)
Plant Physiology 125, 1473-1484
   Abstract »    Full Text »
Fortified Foods and Phytoremediation. Two Sides of the Same Coin.
M. L. Guerinot and D. E. Salt (2001)
Plant Physiology 125, 164-167
   Full Text »
Overexpression of Mitochondrial Citrate Synthase in Arabidopsis thaliana Improved Growth on a Phosphorus-Limited Soil.
H. Koyama, A. Kawamura, T. Kihara, T. Hara, E. Takita, and D. Shibata (2000)
Plant Cell Physiol. 41, 1030-1037
   Abstract »    Full Text »    PDF »
Pattern of Aluminum-Induced Secretion of Organic Acids Differs between Rye and Wheat.
X. F. Li, J. F. Ma, and H. Matsumoto (2000)
Plant Physiology 123, 1537-1544
   Abstract »    Full Text »
Expression of Aluminum-Induced Genes in Transgenic Arabidopsis Plants Can Ameliorate Aluminum Stress and/or Oxidative Stress.
B. Ezaki, R. C. Gardner, Y. Ezaki, and H. Matsumoto (2000)
Plant Physiology 122, 657-666
   Abstract »    Full Text »    PDF »
Early Copper-Induced Leakage of K+ from Arabidopsis Seedlings Is Mediated by Ion Channels and Coupled to Citrate Efflux.
A. S. Murphy, W. R. Eisinger, J. E. Shaff, L. V. Kochian, and L. Taiz (1999)
Plant Physiology 121, 1375-1382
   Abstract »    Full Text »
Use of plant roots for phytoremediation and molecular farming.
D. Gleba, N. V. Borisjuk, L. G. Borisjuk, R. Kneer, A. Poulev, M. Skarzhinskaya, S. Dushenkov, S. Logendra, Y. Y. Gleba, and I. Raskin (1999)
PNAS 96, 5973-5977
   Abstract »    Full Text »    PDF »
Transgenic plants for tropical regions: Some considerations about their development and their transfer to the small farmer.
L. Herrera-Estrella (1999)
PNAS 96, 5978-5981
   Abstract »    Full Text »    PDF »
Oxalate Exudation by Taro in Response to Al.
Z. Ma and S. C. Miyasaka (1998)
Plant Physiology 118, 861-865
   Abstract »    Full Text »
High Aluminum Resistance in Buckwheat . I. Al-induced Specific Secretion of Oxalic Acid from Root Tips.
S. Jian Zheng, J. Feng Ma, and H. Matsumoto (1998)
Plant Physiology 117, 745-751
   Abstract »    Full Text »
Aluminum-Resistant Arabidopsis Mutants That Exhibit Altered Patterns of Aluminum Accumulation and Organic Acid Release from Roots.
P. B. Larsen, J. Degenhardt, C.-Y. Tai, L. M. Stenzler, S. H. Howell, and L. V. Kochian (1998)
Plant Physiology 117, 9-17
   Abstract »    Full Text »
Overexpression of the Saccharomyces cerevisiae Magnesium Transport System Confers Resistance to Aluminum Ion.
C. W. MacDiarmid and R. C. Gardner (1998)
J. Biol. Chem. 273, 1727-1732
   Abstract »    Full Text »    PDF »



To Advertise     Find Products


Science. ISSN 0036-8075 (print), 1095-9203 (online)