Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 23 May 1997:
Vol. 276. no. 5316, pp. 1230 - 1233
DOI: 10.1126/science.276.5316.1230
Prev | Table of Contents | Next

Reports

Direct Measurement of Angles Between Bond Vectors in High-Resolution NMR

Bernd Reif, Mirko Hennig, Christian Griesinger *

Angles between two interatomic vectors are measured for structure elucidation in solution nuclear magnetic resonance (NMR). The angles can be determined directly by using the effects of dipole-dipole cross-correlated relaxation of double-quantum and zero-quantum coherences. The measured rates can be directly related to the angular geometry without need for calibration of a Karplus-type curve, as is the case for scalar coupling measurements, and depend only on the rotational correlation time of the molecule as an empirical parameter. This makes the determination of torsional angles independent from the measurement of coupling constants. The two interatomic vectors can in principle be arbitrarily far apart. The method was demonstrated on the measurement of the peptide backbone angle psi  in the protein rhodniin, which is difficult to determine in solution by NMR spectroscopy.

Institut für Organische Chemie, Marie-Curie-Straße 11, Universität Frankfurt, D-60439 Frankfurt, Germany.
*   To whom correspondence should be addressed. E-mail: cigr{at}krypton.org.chemie.uni-frankfurt.de

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Rapid protein fold determination using unassigned NMR data.
J. Meiler and D. Baker (2003)
PNAS 100, 15404-15409
   Abstract »    Full Text »    PDF »
Reduced-dimensionality NMR spectroscopy for high-throughput protein resonance assignment.
T. Szyperski, D. C. Yeh, D. K. Sukumaran, H. N. B. Moseley, and G. T. Montelione (2002)
PNAS 99, 8009-8014
   Abstract »    Full Text »    PDF »
High-resolution NMR of encapsulated proteins dissolved in low-viscosity fluids.
A. J. Wand, M. R. Ehrhardt, and P. F. Flynn (1998)
PNAS 95, 15299-15302
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)