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Science 2 May 1997:
Vol. 276. no. 5313, pp. 812 - 816
DOI: 10.1126/science.276.5313.812
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Reports

Light-Induced Structural Changes in Photosynthetic Reaction Center: Implications for Mechanism of Electron-Proton Transfer

M. H. B. Stowell, * T. M. McPhillips, dagger D. C. Rees, ddagger S. M. Soltis, E. Abresch, G. Feher ddagger

High resolution x-ray diffraction data from crystals of the Rhodobacter sphaeroides photosynthetic reaction center (RC) have been collected at cryogenic temperature in the dark and under illumination, and the structures were refined at 2.2 and 2.6 angstrom resolution, respectively. In the charge-separated D+QAQB- state (where D is the primary electron donor (a bacteriochlorophyll dimer), and QA and QB are the primary and secondary quinone acceptors, respectively), QB- is located approximately 5 angstroms from the QB position in the charge-neutral (DQAQB) state, and has undergone a 180° propeller twist around the isoprene chain. A model based on the difference between the two structures is proposed to explain the observed kinetics of electron transfer from QA-QB to QAQB- and the relative binding affinities of the different ubiquinone species in the QB pocket. In addition, several water channels (putative proton pathways) leading from the QB pocket to the surface of the RC were delineated, one of which leads directly to the membrane surface.

M. H. B. Stowell, T. M. McPhillips, D. C. Rees, Division of Chemistry and Chemical Engineering, 147-75CH, California Institute of Technology, Pasadena, CA 91125, USA.
S. M. Soltis, Stanford Synchrotron Radiation Laboratory, P.O. Box 4349, Bin 69, Stanford, CA 94309, USA.
E. Abresch, G. Feher, Department of Physics, 0319, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0319, USA.
*   Present address: Medical Research Council, Laboratory of Molecular Biology, Cambridge CB2 2QH, UK.

dagger    Present address: Stanford Synchrotron Radiation Laboratory, P.O. Box 4349, Bin 69, Stanford, CA 94309, USA.

ddagger    To whom correspondence should be addressed.

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