Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 2 May 1997:
Vol. 276. no. 5313, pp. 809 - 812
DOI: 10.1126/science.276.5313.809
Prev | Table of Contents | Next

Reports

Nucleosome Mobility and the Maintenance of Nucleosome Positioning

Michael J. Pazin, Purnima Bhargava, * E. Peter Geiduschek, James T. Kadonaga dagger

To study nucleosome mobility and positioning, the R3 lac repressor was used with an adenosine triphosphate (ATP)-dependent chromatin assembly system to establish the positioning of five nucleosomes, with one nucleosome located between two R3 lac operators. When R3 protein was dissociated from DNA with isopropyl beta -D-thiogalactopyranoside, the R3-induced nucleosome positions remained unchanged for at least 60 minutes in the absence of ATP but rearranged within 15 minutes in the presence of ATP. These results suggest that nucleosomes are dynamic and mobile rather than static and that a DNA binding factor is continuously required for the maintenance of nucleosome positioning.

Department of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla, CA 92093.
*   Permanent address: Center for Cellular and Molecular Biology, Uppal Road, Tarnaka, Hyderabad 500007, India.

dagger    To whom correspondence should be addressed. E-mail: jkadonaga{at}ucsd.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Mi2{beta} Shows Chromatin Enzyme Specificity by Erasing a DNase I-hypersensitive Site Established by ACF.
H. Ishii, H. Du, Z. Zhang, A. Henderson, R. Sen, and M. J. Pazin (2009)
J. Biol. Chem. 284, 7533-7541
   Abstract »    Full Text »    PDF »
Histone Modifications, but Not Nucleosomal Positioning, Correlate with Major Histocompatibility Complex Class I Promoter Activity in Different Tissues In Vivo.
A. S. Kotekar, J. D. Weissman, A. Gegonne, H. Cohen, and D. S. Singer (2008)
Mol. Cell. Biol. 28, 7323-7336
   Abstract »    Full Text »    PDF »
A barrier nucleosome model for statistical positioning of nucleosomes throughout the yeast genome.
T. N. Mavrich, I. P. Ioshikhes, B. J. Venters, C. Jiang, L. P. Tomsho, J. Qi, S. C. Schuster, I. Albert, and B. F. Pugh (2008)
Genome Res. 18, 1073-1083
   Abstract »    Full Text »    PDF »
Chromatin Structure and Expression of a Gene Transcribed by RNA Polymerase III Are Independent of H2A.Z Deposition.
A. G. Arimbasseri and P. Bhargava (2008)
Mol. Cell. Biol. 28, 2598-2607
   Abstract »    Full Text »    PDF »
Positioned Nucleosomes Due to Sequential Remodeling of the Yeast U6 Small Nuclear RNA Chromatin Are Essential for Its Transcriptional Activation.
S. Shivaswamy and P. Bhargava (2006)
J. Biol. Chem. 281, 10461-10472
   Abstract »    Full Text »    PDF »
In Vitro Assembly of the Characteristic Chromatin Organization at the Yeast PHO5 Promoter by a Replication-independent Extract System.
P. Korber and W. Horz (2004)
J. Biol. Chem. 279, 35113-35120
   Abstract »    Full Text »    PDF »
High-Level Activation of Transcription of the Yeast U6 snRNA Gene in Chromatin by the Basal RNA Polymerase III Transcription Factor TFIIIC.
S. Shivaswamy, G. A. Kassavetis, and P. Bhargava (2004)
Mol. Cell. Biol. 24, 3596-3606
   Abstract »    Full Text »    PDF »
Combinatorial Control of DNase I-hypersensitive Site Formation and Erasure by Immunoglobulin Heavy Chain Enhancer-binding Proteins.
H. Ishii, R. Sen, and M. J. Pazin (2004)
J. Biol. Chem. 279, 7331-7338
   Abstract »    Full Text »    PDF »
Properties of Ets-1 Binding to Chromatin and Its Effect on Platelet Factor 4 Gene Expression.
J. Lu, M. J. Pazin, and K. Ravid (2004)
Mol. Cell. Biol. 24, 428-441
   Abstract »    Full Text »    PDF »
Dynamic Properties of Nucleosomes during Thermal and ATP-Driven Mobilization.
A. Flaus and T. Owen-Hughes (2003)
Mol. Cell. Biol. 23, 7767-7779
   Abstract »    Full Text »    PDF »
Constitutive DNase I Hypersensitivity of p53-Regulated Promoters.
C. D. Braastad, Z. Han, and E. A. Hendrickson (2003)
J. Biol. Chem. 278, 8261-8268
   Abstract »    Full Text »    PDF »
LCR-regulated transgene expression levels depend on the Oct-1 site in the AT-rich region of beta -globin intron-2.
R. R. Bharadwaj, C. D. Trainor, P. Pasceri, and J. Ellis (2003)
Blood 101, 1603-1610
   Abstract »    Full Text »    PDF »
Strand pairing by Rad54 and Rad51 is enhanced by chromatin.
V. Alexiadis and J. T. Kadonaga (2002)
Genes & Dev. 16, 2767-2771
   Abstract »    Full Text »    PDF »
Nucleosome mobilization and positioning by ISWI-containing chromatin-remodeling factors.
G. Langst and P. B. Becker (2002)
J. Cell Sci. 114, 2561-2568
   Abstract »    Full Text »    PDF »
Biochemical Analysis of Chromatin Containing Recombinant Drosophila Core Histones.
M. E. Levenstein and J. T. Kadonaga (2002)
J. Biol. Chem. 277, 8749-8754
   Abstract »    Full Text »    PDF »
In Vivo Changes of Nucleosome Positioning in the Pretranscription State.
E. Di Mauro, L. Verdone, B. Chiappini, and M. Caserta (2002)
J. Biol. Chem. 277, 7002-7009
   Abstract »    Full Text »    PDF »
Distinct Domains of Erythroid Kruppel-Like Factor Modulate Chromatin Remodeling and Transactivation at the Endogenous {beta}-Globin Gene Promoter.
R. C. Brown, S. Pattison, J. van Ree, E. Coghill, A. Perkins, S. M. Jane, and J. M. Cunningham (2002)
Mol. Cell. Biol. 22, 161-170
   Abstract »    Full Text »    PDF »
Nucleosomes Are Translationally Positioned on the Active Allele and Rotationally Positioned on the Inactive Allele of the HPRT Promoter.
C. Chen and T. P. Yang (2001)
Mol. Cell. Biol. 21, 7682-7695
   Abstract »    Full Text »    PDF »
Poly(dA-dT) Promoter Elements Increase the Equilibrium Accessibility of Nucleosomal DNA Target Sites.
J. D. Anderson and J. Widom (2001)
Mol. Cell. Biol. 21, 3830-3839
   Abstract »    Full Text »
A Necessary Good: Nuclear Hormone Receptors and Their Chromatin Templates.
F. D. Urnov and A. P. Wolffe (2001)
Mol. Endocrinol. 15, 1-16
   Full Text »
ATP-Dependent Chromatin-Remodeling Complexes.
M. Vignali, A. H. Hassan, K. E. Neely, and J. L. Workman (2000)
Mol. Cell. Biol. 20, 1899-1910
   Full Text »
Selective Nucleosome Disruption by Drugs That Bind in the Minor Groove of DNA.
D. J. Fitzgerald and J. N. Anderson (1999)
J. Biol. Chem. 274, 27128-27138
   Abstract »    Full Text »    PDF »
The Chromatin Structure of the Long Control Region of Human Papillomavirus Type 16 Represses Viral Oncoprotein Expression.
W. Stünkel and H.-U. Bernard (1999)
J. Virol. 73, 1918-1930
   Abstract »    Full Text »
Stable Remodeling of Tailless Nucleosomes by the Human SWI-SNF Complex.
J. R. Guyon, G. J. Narlikar, S. Sif, and R. E. Kingston (1999)
Mol. Cell. Biol. 19, 2088-2097
   Abstract »    Full Text »    PDF »
Promoter Structure and Transcriptional Activation with Chromatin Templates Assembled In Vitro. A SINGLE Gal4-VP16 DIMER BINDS TO CHROMATIN OR TO DNA WITH COMPARABLE AFFINITY.
M. J. Pazin, J. W. Hermann, and J. T. Kadonaga (1998)
J. Biol. Chem. 273, 34653-34660
   Abstract »    Full Text »    PDF »
Xenopus TFIIIA Gene Transcription Is Dependent on cis-Element Positioning and Chromatin Structure.
S. L. Pfaff and W. L. Taylor (1998)
Mol. Cell. Biol. 18, 3811-3818
   Abstract »    Full Text »
The Position and Length of the Steroid-Dependent Hypersensitive Region in the Mouse Mammary Tumor Virus Long Terminal Repeat Are Invariant despite Multiple Nucleosome B Frames.
G. Fragoso, W. D. Pennie, S. John, and G. L. Hager (1998)
Mol. Cell. Biol. 18, 3633-3644
   Abstract »    Full Text »
Nucleosome positioning by the winged helix transcription factor HNF3.
E. Y. Shim, C. Woodcock, and K. S. Zaret (1998)
Genes & Dev. 12, 5-10
   Abstract »    Full Text »
Histone acetyltransferases regulate HIV-1 enhancer activity in vitro.
P. L. Sheridan, T. P. Mayall, E. Verdin, and K. A. Jones (1997)
Genes & Dev. 11, 3327-3340
   Abstract »    Full Text »    PDF »
Chromatin Remodeling and the Control of Gene Expression.
C. Wu (1997)
J. Biol. Chem. 272, 28171-28174
   Full Text »    PDF »
Activation of RNA polymerase II by topologically linked DNA-tracking proteins.
M. Ouhammouch, M. H. Sayre, J. T. Kadonaga, and E. P. Geiduschek (1997)
PNAS 94, 6718-6723
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)