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Science 25 April 1997:
Vol. 276. no. 5312, pp. 604 - 607
DOI: 10.1126/science.276.5312.604
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Reports

Identification of alpha -Fodrin as a Candidate Autoantigen in Primary Sjögren's Syndrome

Norio Haneji, * Takanori Nakamura, * Koji Takio, Kumiko Yanagi, Hiroyuki Higashiyama, Ichiro Saito, Sumihare Noji, Hiromu Sugino, Yoshio Hayashi dagger

It is unclear whether organ-specific autoantigens are critical for the development of primary Sjögren's syndrome (SS). A 120-kilodalton organ-specific autoantigen was purified from salivary gland tissues of an NFS/sld mouse model of human SS. The amino-terminal residues were identical to those of the human cytoskeletal protein alpha -fodrin. The purified antigen induced proliferative T cell responses and production of interleukin-2 and interferon-gamma in vitro. Neonatal immunization with the 120-kilodalton antigen prevented the disease in mice. Sera from patients with SS reacted positively with purified antigen and recombinant human alpha -fodrin protein, whereas those from patients with systemic lupus erythematosus and rheumatoid arthritis did not. Thus, the immune response to 120-kilodalton alpha -fodrin could be important in the initial development of primary SS.

N. Haneji, K. Yanagi, H. Higashiyama, I. Saito, Y. Hayashi, Department of Pathology, Tokushima University School of Dentistry, 3 Kuramotocho, Tokushima 770, Japan.
T. Nakamura and H. Sugino, Institute for Enzyme Research, University of Tokushima, 3 Kuramotocho, Tokushima 770, Japan.
K. Takio, Division of Biomolecular Characterization, The Institute of Physical and Chemical Research, Wako, Saitama 351-01, Japan.
S. Noji, Division of Biological Industry, University of Tokushima, Jyosanjima, Tokushima 770, Japan.
*   These authors contributed equally to this study.

dagger    To whom correspondence should be addressed. E-mail: hayashi{at}dent.tokushima-u.ac.jp

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