Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 18 April 1997:
Vol. 276. no. 5311, pp. 409 - 411
DOI: 10.1126/science.276.5311.409
Prev | Table of Contents | Next

Reports

Effect of Transmembrane and Cytoplasmic Domains of IgE on the IgE Response

Gernot Achatz, * Lars Nitschke, Marinus C. Lamers dagger

B cells use immunoglobulin M (IgM) and IgD as antigen receptors, but after contact with antigen they can switch and use IgG, IgA, or IgE. In mice lacking the transmembrane and cytoplasmic domains of IgE, serum IgE is reduced by more than 95 percent and, after immunization, specific responses are negligible. In mice lacking most of the cytoplasmic tail of IgE, serum IgE levels are reduced by 50 percent and specific responses are reduced by 40 to 80 percent, without a clear secondary response. Thus, membrane expression is indispensable for IgE secretion in vivo, and the cytoplasmic tail influences the degree and quality of the response.

Max-Planck-Institut für Immunbiologie, Stübeweg 51, D-79108 Freiburg, Germany.
*   Present address: Institut für Genetik und Allgemeine Biologie, Hellbrunnerstraße 34, A-5020 Salzburg, Austria.

dagger    To whom correspondence should be addressed. E-mail: lamers{at}immunbio.mpg.de

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Suppression of IgE B Cells and IgE Binding to Fc{epsilon}RI by Gene Therapy with Single-Chain Anti-IgE.
T. Ota, M. Aoki-Ota, B. H. Duong, and D. Nemazee (2009)
J. Immunol. 182, 8110-8117
   Abstract »    Full Text »    PDF »
PLC-{gamma}2 is essential for formation and maintenance of memory B cells.
M. Hikida, S. Casola, N. Takahashi, T. Kaji, T. Takemori, K. Rajewsky, and T. Kurosaki (2009)
J. Exp. Med. 206, 681-689
   Abstract »    Full Text »    PDF »
Targeting the Extracellular Membrane-Proximal Domain of Membrane-Bound IgE by Passive Immunization Blocks IgE Synthesis In Vivo.
S. Feichtner, D. Infuhr, G. Achatz-Straussberger, D. Schmid, A. Karnowski, M. Lamers, C. Rhyner, R. Crameri, and G. Achatz (2008)
J. Immunol. 180, 5499-5505
   Abstract »    Full Text »    PDF »
Soluble CD23 Monomers Inhibit and Oligomers Stimulate IGE Synthesis in Human B Cells.
N. McCloskey, J. Hunt, R. L. Beavil, M. R. Jutton, G. J. Grundy, E. Girardi, S. M. Fabiane, D. J. Fear, D. H. Conrad, B. J. Sutton, et al. (2007)
J. Biol. Chem. 282, 24083-24091
   Abstract »    Full Text »    PDF »
Enhancement and suppression of signaling by the conserved tail of IgG memory-type B cell antigen receptors.
K. Horikawa, S. W. Martin, S. L. Pogue, K. Silver, K. Peng, K. Takatsu, and C. C. Goodnow (2007)
J. Exp. Med. 204, 759-769
   Abstract »    Full Text »    PDF »
IgG1 B cell receptor signaling is inhibited by CD22 and promotes the development of B cells whose survival is less dependent on Ig{alpha}/{beta}.
A. Waisman, M. Kraus, J. Seagal, S. Ghosh, D. Melamed, J. Song, Y. Sasaki, S. Classen, C. Lutz, F. Brombacher, et al. (2007)
J. Exp. Med. 204, 747-758
   Abstract »    Full Text »    PDF »
Augmentation of Signaling through BCR Containing IgE but not That Containing IgA Due to Lack of CD22-Mediated Signal Regulation.
M. Sato, T. Adachi, and T. Tsubata (2007)
J. Immunol. 178, 2901-2907
   Abstract »    Full Text »    PDF »
The Extracellular Membrane-Proximal Domain of Human Membrane IgE Controls Apoptotic Signaling of the B Cell Receptor in the Mature B Cell Line A20.
M. Poggianella, M. Bestagno, and O. R. Burrone (2006)
J. Immunol. 177, 3597-3605
   Abstract »    Full Text »    PDF »
HS1-Associated Protein X-1 Interacts with Membrane-Bound IgE: Impact on Receptor-Mediated Internalization.
I. Oberndorfer, D. Schmid, R. Geisberger, G. Achatz-Straussberger, R. Crameri, M. Lamers, and G. Achatz (2006)
J. Immunol. 177, 1139-1145
   Abstract »    Full Text »    PDF »
Differential T Cell-Mediated Regulation of CD23 (Fc{epsilon}RII) in B Cells and Follicular Dendritic Cells..
S. Sukumar, D. H. Conrad, A. K. Szakal, and J. G. Tew (2006)
J. Immunol. 176, 4811-4817
   Abstract »    Full Text »    PDF »
Visualization of the Genesis and Fate of Isotype-switched B Cells during a Primary Immune Response.
K. A. Pape, V. Kouskoff, D. Nemazee, H. L. Tang, J. G. Cyster, L. E. Tze, K. L. Hippen, T. W. Behrens, and M. K. Jenkins (2003)
J. Exp. Med. 197, 1677-1687
   Abstract »    Full Text »    PDF »
Isotype Control of B Cell Signaling.
K. Silver and R. J. Cornall (2003)
Sci. STKE 2003, pe21
   Abstract »    Full Text »    PDF »
Fate of the mutated IgG2 heavy chain: lack of expression of mutated membrane-bound IgG2 on the B cell surface in selective IgG2 deficiency.
T. Terada, H. Kaneko, T. Fukao, H. Tashita, A. L. Li, M. Takemura, and N. Kondo (2001)
Int. Immunol. 13, 249-256
   Abstract »    Full Text »    PDF »
Inactivation of the (&bgr;)-catenin gene by Wnt1-Cre-mediated deletion results in dramatic brain malformation and failure of craniofacial development.
V Brault, R Moore, S Kutsch, M Ishibashi, D. Rowitch, A. McMahon, L Sommer, O Boussadia, and R Kemler (2001)
Development 128, 1253-1264
   Abstract »    PDF »
The Road to the Production of IgE Is Long and Winding.
A. KARNOWSKI, P. YU, G. ACHATZ, and M. C. LAMERS (2000)
Am. J. Respir. Crit. Care Med. 162, S71-75
   Full Text »    PDF »
Gene Dose-Dependent Maturation and Receptor Editing of B Cells Expressing Immunoglobulin (Ig)g1 or Igm/Igg1 Tail Antigen Receptors.
S. L. Pogue and C. C. Goodnow (2000)
J. Exp. Med. 191, 1031-1044
   Abstract »    Full Text »    PDF »
Differences Between IL-4- and IL-4 Receptor {alpha}-Deficient Mice in Chronic Leishmaniasis Reveal a Protective Role for IL-13 Receptor Signaling.
M. Mohrs, B. Ledermann, G. Kohler, A. Dorfmuller, A. Gessner, and F. Brombacher (1999)
J. Immunol. 162, 7302-7308
   Abstract »    Full Text »    PDF »
The Roles of gamma 1 Heavy Chain Membrane Expression and Cytoplasmic Tail in IgG1 Responses.
T. Kaisho, F. Schwenk, and K. Rajewsky (1997)
Science 276, 412-415
   Abstract »    Full Text »
Association of SWAP-70 with the B cell antigen receptor complex.
L. Masat, J. Caldwell, R. Armstrong, H. Khoshnevisan, R. Jessberger, B. Herndier, M. Wabl, and D. Ferrick (2000)
PNAS 97, 2180-2184
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)