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Science 4 April 1997:
Vol. 276. no. 5309, pp. 114 - 117
DOI: 10.1126/science.276.5309.114
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Reports

Coordination of Drosophila Metamorphosis by Two Ecdysone-Induced Nuclear Receptors

Kevin P. White, Patrick Hurban, Toshiki Watanabe, * David S. Hogness dagger

The functions of the ecdysone-induced DHR3 and E75B orphan nuclear receptors in the early stages of Drosophila metamorphosis were investigated. DHR3 represses the ecdysone induction of early genes turned on by the pulse of ecdysone that triggers metamorphosis. It also induces beta FTZF1, an orphan nuclear receptor that is essential for the appropriate response to the subsequent prepupal pulse of ecdysone. The E75B receptor, which lacks a complete DNA binding domain, inhibits this inductive function by forming a complex with DHR3 on the beta FTZF1 promoter, thereby providing a timing mechanism for beta FTZF1 induction that is dependent on the disappearance of E75B.

Departments of Developmental Biology and Biochemistry, Beckman Center B300, Stanford University School of Medicine, Stanford, CA 94305-5427, USA.
*   Present address: Laboratory of Molecular Biology of Marine Organisms, Ocean Research Institute, University of Tokyo, 1-15-1 Minamidai, Nakano-ku, Tokyo 164, Japan.

dagger    To whom correspondence should be addressed.

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