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Science 14 March 1997:
Vol. 275. no. 5306, pp. 1655 - 1657
DOI: 10.1126/science.275.5306.1655
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Reports

RNA Polymerase beta ' Subunit: A Target of DNA Binding-Independent Activation

Alita Miller, * Douglas Wood, Richard H. Ebright, Lucia B. Rothman-Denes dagger

The bacteriophage N4 single-stranded DNA binding protein (N4SSB) activates transcription by the Escherichia coli sigma 70-RNA polymerase at N4 late promoters. Here it is shown that the single-stranded DNA binding activity of N4SSB is not required for transcriptional activation. N4SSB interacts with the carboxyl terminus of the RNA polymerase beta ' subunit in a region that is highly conserved in the largest subunits of prokaryotic and eukaryotic RNA polymerases.

A. Miller, Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL 60637, USA.
D. Wood and L. B. Rothman-Denes, Department of Molecular Genetics and Cell Biology, University of Chicago, 920 East 58 Street, Chicago, IL 60637, USA.
R. H. Ebright, Department of Chemistry and Waksman Institute, Rutgers University, New Brunswick, NJ 08855, USA.
*   Present address: L'Institut de Genétique et de Biologie Moléculaire et Cellulaire, B. P. 163, 67404 Illkirch, France.

dagger    To whom correspondence should be addressed.

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