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Science 28 February 1997:
Vol. 275. no. 5304, pp. 1308 - 1311
DOI: 10.1126/science.275.5304.1308
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Reports

Formation of Actin Stress Fibers and Focal Adhesions Enhanced by Rho-Kinase

Mutsuki Amano, Kazuyasu Chihara, Kazushi Kimura, Yuko Fukata, Nao Nakamura, Yoshiharu Matsuura, Kozo Kaibuchi *

The small guanosine triphosphatase (GTPase) Rho is implicated in the formation of stress fibers and focal adhesions in fibroblasts stimulated by extracellular signals such as lysophosphatidic acid (LPA). Rho-kinase is activated by Rho and may mediate some biological effects of Rho. Microinjection of the catalytic domain of Rho-kinase into serum-starved Swiss 3T3 cells induced the formation of stress fibers and focal adhesions, whereas microinjection of the inactive catalytic domain, the Rho-binding domain, or the pleckstrin-homology domain inhibited the LPA-induced formation of stress fibers and focal adhesions. Thus, Rho-kinase appears to mediate signals from Rho and to induce the formation of stress fibers and focal adhesions.

M. Amano, K. Chihara, Y. Fukata, N. Nakamura, K. Kaibuchi, Division of Signal Transduction, Nara Institute of Science and Technology, Ikoma 630-01, Japan.
K. Kimura, Division of Signal Transduction, Nara Institute of Science and Technology, Ikoma 630-01, Japan, and Second Department of Anatomy, Kyoto University Faculty of Medicine, Kyoto 606, Japan.
Y. Matsuura, Department of Virology II, National Institute of Health, Tokyo 162, Japan.
*   To whom correspondence should be addressed.

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Uncoordinated regulation of stress fibers and focal adhesions by DAP kinase.
J.-C. Kuo, J.-R. Lin, J. M. Staddon, H. Hosoya, and R.-H. Chen (2003)
J. Cell Sci. 116, 4777-4790
   Abstract »    Full Text »    PDF »
Asymmetric Distribution of Myosin IIB in Migrating Endothelial Cells Is Regulated by a rho-dependent Kinase and Contributes to Tail Retraction.
J. Kolega (2003)
Mol. Biol. Cell 14, 4745-4757
   Abstract »    Full Text »    PDF »
Parallel Coiled-coil Association of the RhoA-binding Domain in Rho-kinase.
T. Shimizu, K. Ihara, R. Maesaki, M. Amano, K. Kaibuchi, and T. Hakoshima (2003)
J. Biol. Chem. 278, 46046-46051
   Abstract »    Full Text »    PDF »
ROCK-generated contractility regulates breast epithelial cell differentiation in response to the physical properties of a three-dimensional collagen matrix.
M. A. Wozniak, R. Desai, P. A. Solski, C. J. Der, and P. J. Keely (2003)
J. Cell Biol. 163, 583-595
   Abstract »    Full Text »    PDF »
Nitric Oxide Inhibits Stretch-Induced MAPK Activation in Mesangial Cells Through RhoA Inactivation.
J. C. Krepinsky, A. J. Ingram, D. Tang, D. Wu, L. Liu, and J. W. Scholey (2003)
J. Am. Soc. Nephrol. 14, 2790-2800
   Abstract »    Full Text »    PDF »
Higher Expression of RhoC Is Related to Invasiveness in Non-Small Cell Lung Carcinoma.
Y. Shikada, I. Yoshino, T. Okamoto, S. Fukuyama, T. Kameyama, and Y. Maehara (2003)
Clin. Cancer Res. 9, 5282-5286
   Abstract »    Full Text »    PDF »
CCK-A receptor activates RhoA through G{alpha}12/13 in NIH3T3 cells.
S. L. Le Page, Y. Bi, and J. A. Williams (2003)
Am J Physiol Cell Physiol 285, C1197-C1206
   Abstract »    Full Text »    PDF »
Hypoxia Inhibits Myosin Phosphatase in Pulmonary Arterial Smooth Muscle Cells: Role of Rho-Kinase.
Z. Wang, M. C. Lanner, N. Jin, D. Swartz, L. Li, and R. A. Rhoades (2003)
Am. J. Respir. Cell Mol. Biol. 29, 465-471
   Abstract »    Full Text »    PDF »
Caspase activation during phorbol ester-induced apoptosis requires ROCK-dependent myosin-mediated contraction.
J.-M. Lai, C.-L. Hsieh, and Z.-F. Chang (2003)
J. Cell Sci. 116, 3491-3501
   Abstract »    Full Text »    PDF »
Hyaluronan-mediated CD44 Interaction with RhoGEF and Rho Kinase Promotes Grb2-associated Binder-1 Phosphorylation and Phosphatidylinositol 3-Kinase Signaling Leading to Cytokine (Macrophage-Colony Stimulating Factor) Production and Breast Tumor Progression.
L. Y. W. Bourguignon, P. A. Singleton, H. Zhu, and F. Diedrich (2003)
J. Biol. Chem. 278, 29420-29434
   Abstract »    Full Text »    PDF »
Contractile activity and smooth muscle {alpha}-actin organization in thrombin-induced human lung myofibroblasts.
G. S. Bogatkevich, E. Tourkina, C. S. Abrams, R. A. Harley, R. M. Silver, and A. Ludwicka-Bradley (2003)
Am J Physiol Lung Cell Mol Physiol 285, L334-L343
   Abstract »    Full Text »    PDF »
The RhoA Effector mDia Is Induced During T Cell Activation and Regulates Actin Polymerization and Cell Migration in T Lymphocytes.
M. Vicente-Manzanares, M. Rey, M. Perez-Martinez, M. Yanez-Mo, D. Sancho, J. R. Cabrero, O. Barreiro, H. de la Fuente, K. Itoh, and F. Sanchez-Madrid (2003)
J. Immunol. 171, 1023-1034
   Abstract »    Full Text »    PDF »
Rho Kinase Promotes Alloimmune Responses by Regulating the Proliferation and Structure of T Cells.
P.-L. Tharaux, R. C. Bukoski, P. N. Rocha, S. D. Crowley, P. Ruiz, C. Nataraj, D. N. Howell, K. Kaibuchi, R. F. Spurney, and T. M. Coffman (2003)
J. Immunol. 171, 96-105
   Abstract »    Full Text »    PDF »
Differential regulation of junctional complex assembly in renal epithelial cell lines.
S. Gopalakrishnan, M. A. Hallett, S. J. Atkinson, and James. A. Marrs (2003)
Am J Physiol Cell Physiol 285, C102-C111
   Abstract »    Full Text »    PDF »
Effects of Rho Kinase and Actin Stress Fibers on Sustained Extracellular Signal-Regulated Kinase Activity and Activation of G1 Phase Cyclin-Dependent Kinases.
K. Roovers and R. K. Assoian (2003)
Mol. Cell. Biol. 23, 4283-4294
   Abstract »    Full Text »    PDF »
A role for LIM kinase in cancer invasion.
K. Yoshioka, V. Foletta, O. Bernard, and K. Itoh (2003)
PNAS 100, 7247-7252
   Abstract »    Full Text »    PDF »
{alpha}-Adducin dissociates from F-actin and spectrin during platelet activation.
K. L. Barkalow, J. E. Italiano Jr., D. E. Chou, Y. Matsuoka, V. Bennett, and J. H. Hartwig (2003)
J. Cell Biol. 161, 557-570
   Abstract »    Full Text »    PDF »
The formin-homology-domain-containing protein FHOD1 enhances cell migration.
S. Koka, C. L. Neudauer, X. Li, R. E. Lewis, J. B. McCarthy, and J. J. Westendorf (2003)
J. Cell Sci. 116, 1745-1755
   Abstract »    Full Text »    PDF »


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