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Science 13 December 1996:
Vol. 274. no. 5294, pp. 1903 - 1905
DOI: 10.1126/science.274.5294.1903
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Reports

NF-AT-Driven Interleukin-4 Transcription Potentiated by NIP45

Martin R. Hodge, *dagger Hyung J. Chun, * Jyothi Rengarajan, Aya Alt, Rebecca Lieberson, Laurie H. Glimcher

The induction of cytokine gene transcription is mediated in part by the nuclear factor of activated T cells (NF-AT). Factors involved in the mechanisms of NF-AT-mediated transcription are not well understood. A nuclear factor that interacted with the Rel homology domain (RHD) of NF-ATp was identified with the use of a two-hybrid interaction trap. Designated NIP45 (NF-AT interacting protein), it has minimal similarity to any known genes. Transcripts encoding this factor were enriched in lymphoid tissues and testes. NIP45 synergized with NF-ATp and the proto-oncogene c-Maf to activate the interleukin-4 (IL-4) cytokine promoter; transient overexpression of NIP45 with NF-ATp and c-maf in B lymphoma cells induced measurable endogenous IL-4 protein production. The identification of NIP45 advances our understanding of gene activation of cytokines, critical mediators of the immune response.

Department of Cancer Biology, Harvard School of Public Health and Department of Medicine, Harvard Medical School, Boston, MA 02115, USA.
*   These authors contributed equally and are both considered primary authors.

dagger    Present address: Millennium Pharmaceuticals Inc., Cambridge, MA 02139, USA.

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