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Science 29 November 1996:
Vol. 274. no. 5292, pp. 1514 - 1517
DOI: 10.1126/science.274.5292.1514
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Reports

Sequence Similarity Between the 73-Kilodalton Protein of Mammalian CPSF and a Subunit of Yeast Polyadenylation Factor I

Andreas Jenny, * Lionel Minvielle-Sebastia, Pascal J. Preker, Walter Keller dagger

The 3' ends of most eukaryotic messenger RNAs are generated by endonucleolytic cleavage and polyadenylation. In mammals, the cleavage and polyadenylation specificity factor (CPSF) plays a central role in both steps of the processing reaction. Here, the cloning of the 73-kilodalton subunit of CPSF is reported. Sequence analyses revealed that a yeast protein (Ysh1) was highly similar to the 73-kD polypeptide. Ysh1 constitutes a new subunit of polyadenylation factor I (PFI), which has a role in yeast pre-mRNA 3'-end formation. This finding was unexpected because in contrast to CPSF, PFI is only required for the polyadenylation reaction. These results contribute to the understanding of how 3'-end processing factors may have evolved.

Department of Cell Biology, Biozentrum, University of Basel, CH-4056 Basel, Switzerland. The first three authors contributed equally to this work.
*   Present address: EMBL, Meyerhofstrasse 1, D-69117 Heidelberg, Germany.

dagger    E-mail: Keller2{at}ubaclu.unibas.ch

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