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Science 15 November 1996:
Vol. 274. no. 5290, pp. 1205 - 1208
DOI: 10.1126/science.274.5290.1205
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Reports

Requirement of the Manx Gene for Expression of Chordate Features in a Tailless Ascidian Larva

Billie J. Swalla * and William R. Jeffery dagger

An evolutionary change in development was studied in two closely related ascidian species, one exhibiting a conventional tadpole larva and the other a modified tailless larva. Interspecific hybridization restores chordate features to the tailless larva. The zinc finger gene Manx is expressed in cells that generate chordate features in the tailed species but is down-regulated in the tailless species. Manx expression is restored in hybrid embryos. Antisense oligodeoxynucleotide treatment inhibited Manx expression and chordate features in hybrid embryos, which suggests that Manx is required for development of the chordate larval phenotype in ascidians.

B. J. Swalla, Department of Biology, Vanderbilt University, Nashville, TN 37235, USA, and Station Biologique, Roscoff, France.
W. R. Jeffery, Bodega Marine Laboratory, Bodega Bay, CA 94923, USA; Section of Molecular and Cellular Biology, University of California, Davis, CA 95616, USA; and Station Biologique, Roscoff, France.
*   To whom correspondence should be addressed. E-mail: swallabj{at}ctrvax.vanderbilt.edu

dagger    Present address: Biology Department, Pennsylvania State University, University Park, PA 16802, USA.

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