Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 8 November 1996:
Vol. 274. no. 5289, pp. 1001 - 1005
DOI: 10.1126/science.274.5289.1001
Prev | Table of Contents | Next

Reports

Structure of the p53 Tumor Suppressor Bound to the Ankyrin and SH3 Domains of 53BP2

Svetlana Gorina and Nikola P. Pavletich

Mutations in the p53 tumor suppressor are among the most frequently observed genetic alterations in human cancer and map to the 200-amino acid core domain of the protein. The core domain contains the sequence-specific DNA binding activity and the in vitro 53BP2 protein binding activity of p53. The crystal structure of the p53 core domain bound to the 53BP2 protein, which contains an SH3 (Src homology 3) domain and four ankyrin repeats, revealed that (i) the SH3 domain binds the L3 loop of p53 in a manner distinct from that of previously characterized SH3-polyproline peptide complexes, and (ii) an ankyrin repeat, which forms an L-shaped structure consisting of a beta  hairpin and two alpha  helices, binds the L2 loop of p53. The structure of the complex shows that the 53BP2 binding site on the p53 core domain consists of evolutionarily conserved regions that are frequently mutated in cancer and that it overlaps the site of DNA binding. The six most frequently observed p53 mutations disrupt 53BP2 binding in vitro. The structure provides evidence that the 53BP2-p53 complex forms in vivo and may have a critical role in the p53 pathway of tumor suppression.

Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The Myxoma Virus M-T5 Ankyrin Repeat Host Range Protein Is a Novel Adaptor That Coordinately Links the Cellular Signaling Pathways Mediated by Akt and Skp1 in Virus-Infected Cells.
S. J. Werden, J. Lanchbury, D. Shattuck, C. Neff, M. Dufford, and G. McFadden (2009)
J. Virol. 83, 12068-12083
   Abstract »    Full Text »    PDF »
p53 Pre- and Post-Binding Event Theories Revisited: Stresses Reveal Specific and Dynamic p53-Binding Patterns on the p21 Gene Promoter.
J.-F. Millau, N. Bastien, E. F. Bouchard, and R. Drouin (2009)
Cancer Res. 69, 8463-8471
   Abstract »    Full Text »    PDF »
Insight into the Structural Basis of Pro- and Antiapoptotic p53 Modulation by ASPP Proteins.
J. Ahn, I.-J. L. Byeon, C.-H. Byeon, and A. M. Gronenborn (2009)
J. Biol. Chem. 284, 13812-13822
   Abstract »    Full Text »    PDF »
Apoptosis-stimulating protein of p53 (ASPP2) heterozygous mice are tumor-prone and have attenuated cellular damage-response thresholds.
K. M. Kampa, J. D. Acoba, D. Chen, J. Gay, H. Lee, K. Beemer, E. Padiernos, N. Boonmark, Z. Zhu, A. C. Fan, et al. (2009)
PNAS 106, 4390-4395
   Abstract »    Full Text »    PDF »
Identification of ANKRD11 as a p53 coactivator.
P. M. Neilsen, K. M. Cheney, C.-W. Li, J. D. Chen, J. E. Cawrse, R. B. Schulz, J. A. Powell, R. Kumar, and D. F. Callen (2008)
J. Cell Sci. 121, 3541-3552
   Abstract »    Full Text »    PDF »
Molecular interactions of ASPP1 and ASPP2 with the p53 protein family and the apoptotic promoters PUMA and Bax.
S. Patel, R. George, F. Autore, F. Fraternali, J. E. Ladbury, and P. V. Nikolova (2008)
Nucleic Acids Res. 36, 5139-5151
   Abstract »    Full Text »    PDF »
Molecular basis of the interaction between the antiapoptotic Bcl-2 family proteins and the proapoptotic protein ASPP2.
C. Katz, H. Benyamini, S. Rotem, M. Lebendiker, T. Danieli, A. Iosub, H. Refaely, M. Dines, V. Bronner, T. Bravman, et al. (2008)
PNAS 105, 12277-12282
   Abstract »    Full Text »    PDF »
The Structure and Interactions of the Proline-rich Domain of ASPP2.
S. Rotem, C. Katz, H. Benyamini, M. Lebendiker, D. Veprintsev, S. Rudiger, T. Danieli, and A. Friedler (2008)
J. Biol. Chem. 283, 18990-18999
   Abstract »    Full Text »    PDF »
Structural insights into an equilibrium folding intermediate of an archaeal ankyrin repeat protein.
C. Low, U. Weininger, P. Neumann, M. Klepsch, H. Lilie, M. T. Stubbs, and J. Balbach (2008)
PNAS 105, 3779-3784
   Abstract »    Full Text »    PDF »
NM23-H1 Tumor Suppressor and Its Interacting Partner STRAP Activate p53 Function.
H. Jung, H.-A. Seong, and H. Ha (2007)
J. Biol. Chem. 282, 35293-35307
   Abstract »    Full Text »    PDF »
From the Cover: Quaternary structures of tumor suppressor p53 and a specific p53 DNA complex.
H. Tidow, R. Melero, E. Mylonas, S. M. V. Freund, J. G. Grossmann, J. M. Carazo, D. I. Svergun, M. Valle, and A. R. Fersht (2007)
PNAS 104, 12324-12329
   Abstract »    Full Text »    PDF »
Crystal Structure of the Rac Activator, Asef, Reveals Its Autoinhibitory Mechanism.
K. Murayama, M. Shirouzu, Y. Kawasaki, M. Kato-Murayama, K. Hanawa-Suetsugu, A. Sakamoto, Y. Katsura, A. Suenaga, M. Toyama, T. Terada, et al. (2007)
J. Biol. Chem. 282, 4238-4242
   Abstract »    Full Text »    PDF »
Effects of Oncogenic Mutations and DNA Response Elements on the Binding of p53 to p53-binding Protein 2 (53BP2).
H. Tidow, D. B. Veprintsev, S. M. V. Freund, and A. R. Fersht (2006)
J. Biol. Chem. 281, 32526-32533
   Abstract »    Full Text »    PDF »
From the Cover: Structural basis for understanding oncogenic p53 mutations and designing rescue drugs.
A. C. Joerger, H. C. Ang, and A. R. Fersht (2006)
PNAS 103, 15056-15061
   Abstract »    Full Text »    PDF »
Crystal structure of SV40 large T-antigen bound to p53: interplay between a viral oncoprotein and a cellular tumor suppressor.
W. Lilyestrom, M. G. Klein, R. Zhang, A. Joachimiak, and X. S. Chen (2006)
Genes & Dev. 20, 2373-2382
   Abstract »    Full Text »    PDF »
Genomic Organization, Differential Expression, and Interaction of SQUAMOSA Promoter-Binding-Like Transcription Factors and microRNA156 in Rice.
K. Xie, C. Wu, and L. Xiong (2006)
Plant Physiology 142, 280-293
   Abstract »    Full Text »    PDF »
ASPP2 is a haploinsufficient tumor suppressor that cooperates with p53 to suppress tumor growth..
V. Vives, J. Su, S. Zhong, I. Ratnayaka, E. Slee, R. Goldin, and X. Lu (2006)
Genes & Dev. 20, 1262-1267
   Abstract »    Full Text »    PDF »
E2 Proteins from High- and Low-Risk Human Papillomavirus Types Differ in Their Ability To Bind p53 and Induce Apoptotic Cell Death.
J. L. Parish, A. Kowalczyk, H.-T. Chen, G. E. Roeder, R. Sessions, M. Buckle, and K. Gaston (2006)
J. Virol. 80, 4580-4590
   Abstract »    Full Text »    PDF »
Disruption of G1-phase phospholipid turnover by inhibition of Ca2+-independent phospholipase A2 induces a p53-dependent cell-cycle arrest in G1 phase.
X. H. Zhang, C. Zhao, K. Seleznev, K. Song, J. J. Manfredi, and Z. A. Ma (2006)
J. Cell Sci. 119, 1005-1015
   Abstract »    Full Text »    PDF »
Control of ASPP2/53BP2L Protein Levels by Proteasomal Degradation Modulates p53 Apoptotic Function.
Z. Zhu, J. Ramos, K. Kampa, S. Adimoolam, M. Sirisawad, Z. Yu, D. Chen, L. Naumovski, and C. D. Lopez (2005)
J. Biol. Chem. 280, 34473-34480
   Abstract »    Full Text »    PDF »
Inhibition of the 53BP2S-mediated apoptosis by nuclear factor {kappa}B and Bcl-2 family proteins.
N. Takahashi, S. Kobayashi, S. Kajino, K. Imai, K. Tomoda, S. Shimizu, and T. Okamoto (2005)
Genes Cells 10, 803-811
   Abstract »    Full Text »    PDF »
MxA, a Member of the Dynamin Superfamily, Interacts with the Ankyrin-like Repeat Domain of TRPC.
M. P. Lussier, S. Cayouette, P. K. Lepage, C. L. Bernier, N. Francoeur, M. St-Hilaire, M. Pinard, and G. Boulay (2005)
J. Biol. Chem. 280, 19393-19400
   Abstract »    Full Text »    PDF »
Importance of a Surface Hydrophobic Pocket on Protein Phosphatase-1 Catalytic Subunit in Recognizing Cellular Regulators.
J. A. Gibbons, D. C. Weiser, and S. Shenolikar (2005)
J. Biol. Chem. 280, 15903-15911
   Abstract »    Full Text »    PDF »
Structures of p53 Cancer Mutants and Mechanism of Rescue by Second-site Suppressor Mutations.
A. C. Joerger, H. C. Ang, D. B. Veprintsev, C. M. Blair, and A. R. Fersht (2005)
J. Biol. Chem. 280, 16030-16037
   Abstract »    Full Text »    PDF »
Binding of Rad51 and Other Peptide Sequences to a Promiscuous, Highly Electrostatic Binding Site in p53.
A. Friedler, D. B. Veprintsev, T. Rutherford, K. I. von Glos, and A. R. Fersht (2005)
J. Biol. Chem. 280, 8051-8059
   Abstract »    Full Text »    PDF »
A mutation in NFkB interacting protein 1 results in cardiomyopathy and abnormal skin development in wa3 mice.
B. J. Herron, C. Rao, S. Liu, L. Laprade, J. A. Richardson, E. Olivieri, C. Semsarian, S. E. Millar, L. Stubbs, and D. R. Beier (2005)
Hum. Mol. Genet. 14, 667-677
   Abstract »    Full Text »    PDF »
53BP2 induces apoptosis through the mitochondrial death pathway.
S. Kobayashi, S. Kajino, N. Takahashi, S. Kanazawa, K. Imai, Y. Hibi, H. Ohara, M. Itoh, and T. Okamoto (2005)
Genes Cells 10, 253-260
   Abstract »    Full Text »    PDF »
Genetic and Biochemical Interactions Among Yar1, Ltv1 and RpS3 Define Novel Links Between Environmental Stress and Ribosome Biogenesis in Saccharomyces cerevisiae.
J. W. Loar, R. M. Seiser, A. E. Sundberg, H. J. Sagerson, N. Ilias, P. Zobel-Thropp, E. A. Craig, and D. E. Lycan (2004)
Genetics 168, 1877-1889
   Abstract »    Full Text »    PDF »
An experimentally determined protein folding energy landscape.
C. C. Mello and D. Barrick (2004)
PNAS 101, 14102-14107
   Abstract »    Full Text »    PDF »
Functional Subdomain in the Ankyrin Domain of Tankyrase 1 Required for Poly(ADP-Ribosyl)ation of TRF1 and Telomere Elongation.
H. Seimiya, Y. Muramatsu, S. Smith, and T. Tsuruo (2004)
Mol. Cell. Biol. 24, 1944-1955
   Abstract »    Full Text »    PDF »
ASPP1 and ASPP2: Common Activators of p53 Family Members.
D. Bergamaschi, Y. Samuels, B. Jin, S. Duraisingham, T. Crook, and X. Lu (2004)
Mol. Cell. Biol. 24, 1341-1350
   Abstract »    Full Text »    PDF »
The Crystal Structure of Gankyrin, an Oncoprotein Found in Complexes with Cyclin-dependent Kinase 4, a 19 S Proteasomal ATPase Regulator, and the Tumor Suppressors Rb and p53.
S. Krzywda, A. M. Brzozowski, H. Higashitsuji, J. Fujita, R. Welchman, S. Dawson, R. J. Mayer, and A. J. Wilkinson (2004)
J. Biol. Chem. 279, 1541-1545
   Abstract »    Full Text »    PDF »
Isolation of Temperature-sensitive p53 Mutations from a Comprehensive Missense Mutation Library.
K. Shiraishi, S. Kato, S.-Y. Han, W. Liu, K. Otsuka, M. Sakayori, T. Ishida, M. Takeda, R. Kanamaru, N. Ohuchi, et al. (2004)
J. Biol. Chem. 279, 348-355
   Abstract »    Full Text »    PDF »
Complementary DNA Cloning and Characterization of Rat spergen-2, a Spermatogenic Cell-Specific Gene 2 Encoding a 56-Kilodalton Nuclear Protein Bearing Ankyrin Repeat Motifs.
H. Iida, A. Urasoko, M. Doiguchi, T. Mori, K. Toshimori, and Y. Shibata (2003)
Biol Reprod 69, 421-429
   Abstract »    Full Text »    PDF »
X-ray Crystal Structure of an I{kappa}B{beta}{middle dot}NF-{kappa}B p65 Homodimer Complex.
S. Malek, D.-B. Huang, T. Huxford, S. Ghosh, and G. Ghosh (2003)
J. Biol. Chem. 278, 23094-23100
   Abstract »    Full Text »    PDF »
Degeneracy and Function of the Ubiquitous RVXF Motif That Mediates Binding to Protein Phosphatase-1.
P. Wakula, M. Beullens, H. Ceulemans, W. Stalmans, and M. Bollen (2003)
J. Biol. Chem. 278, 18817-18823
   Abstract »    Full Text »    PDF »
Assembly of the PINCH-ILK-CH-ILKBP complex precedes and is essential for localization of each component to cell-matrix adhesion sites.
Y. Zhang, K. Chen, Y. Tu, A. Velyvis, Y. Yang, J. Qin, and C. Wu (2003)
J. Cell Sci. 115, 4777-4786
   Abstract »    Full Text »    PDF »
Mapping the Proteome of Barrel Medic (Medicago truncatula).
B. S. Watson, V. S. Asirvatham, L. Wang, and L. W. Sumner (2003)
Plant Physiology 131, 1104-1123
   Abstract »    Full Text »    PDF »
Designed to be stable: Crystal structure of a consensus ankyrin repeat protein.
A. Kohl, H. K. Binz, P. Forrer, M. T. Stumpp, A. Pluckthun, and M. G. Grutter (2003)
PNAS 100, 1700-1705
   Abstract »    Full Text »    PDF »
The Cdc42 Binding and Scaffolding Activities of the Fission Yeast Adaptor Protein Scd2.
M. Endo, M. Shirouzu, and S. Yokoyama (2003)
J. Biol. Chem. 278, 843-852
   Abstract »    Full Text »    PDF »
Two sequence motifs from HIF-1alpha bind to the DNA-binding site of p53.
L. O. Hansson, A. Friedler, S. Freund, S. Rudiger, and A. R. Fersht (2002)
PNAS 99, 10305-10309
   Abstract »    Full Text »    PDF »
Vitreous Treatment of Cultured Human RPE Cells Results in Differential Expression of 10 New Genes.
N. Kociok, A. Hueber, P. Esser, U. Schraermeyer, G. Thumann, T. T. Luther, J. Jordan, G. Welsandt, and B. Kirchhof (2002)
Invest. Ophthalmol. Vis. Sci. 43, 2474-2480
   Abstract »    Full Text »    PDF »
Identification of Gasz, an Evolutionarily Conserved Gene Expressed Exclusively in Germ Cells and Encoding a Protein with Four Ankyrin Repeats, a Sterile-{alpha} Motif, and a Basic Leucine Zipper.
W. Yan, A. Rajkovic, M. M. Viveiros, K. H. Burns, J. J. Eppig, and M. M. Matzuk (2002)
Mol. Endocrinol. 16, 1168-1184
   Abstract »    Full Text »    PDF »
The Telomeric Poly(ADP-ribose) Polymerase, Tankyrase 1, Contains Multiple Binding Sites for Telomeric Repeat Binding Factor 1 (TRF1) and a Novel Acceptor, 182-kDa Tankyrase-binding Protein (TAB182).
H. Seimiya and S. Smith (2002)
J. Biol. Chem. 277, 14116-14126
   Abstract »    Full Text »    PDF »
Structure of the 53BP1 BRCT region bound to p53 and its comparison to the Brca1 BRCT structure.
W. S. Joo, P. D. Jeffrey, S. B. Cantor, M. S. Finnin, D. M. Livingston, and N. P. Pavletich (2002)
Genes & Dev. 16, 583-593
   Abstract »    Full Text »    PDF »
CLIPR-59, a new trans-Golgi/TGN cytoplasmic linker protein belonging to the CLIP-170 family.
F. Perez, K. Pernet-Gallay, C. Nizak, H. V. Goodson, T. E. Kreis, and B. Goud (2002)
J. Cell Biol. 156, 631-642
   Abstract »    Full Text »    PDF »
Protein phosphatase 1 - targeted in many directions.
P. T. W. Cohen (2002)
J. Cell Sci. 115, 241-256
   Abstract »    Full Text »    PDF »
A peptide that binds and stabilizes p53 core domain: Chaperone strategy for rescue of oncogenic mutants.
A. Friedler, L. O. Hansson, D. B. Veprintsev, S. M. V. Freund, T. M. Rippin, P. V. Nikolova, M. R. Proctor, S. Rudiger, and A. R. Fersht (2002)
PNAS
   Abstract »    Full Text »    PDF »
Functional Analysis of Asb-1 Using Genetic Modification in Mice.
B. T. Kile, D. Metcalf, S. Mifsud, L. DiRago, N. A. Nicola, D. J. Hilton, and W. S. Alexander (2001)
Mol. Cell. Biol. 21, 6189-6197
   Abstract »    Full Text »    PDF »
AMF1 (GPS2) Modulates p53 Transactivation.
Y.-C. Peng, F. Kuo, D. E. Breiding, Y.-F. Wang, C. P. Mansur, and E. J. Androphy (2001)
Mol. Cell. Biol. 21, 5913-5924
   Abstract »    Full Text »    PDF »
Analysis of Ankyrin Repeats Reveals How a Single Point Mutation in RFXANK Results in Bare Lymphocyte Syndrome.
N. Nekrep, M. Geyer, N. Jabrane-Ferrat, and B. M. Peterlin (2001)
Mol. Cell. Biol. 21, 5566-5576
   Abstract »    Full Text »    PDF »
Conservation of glp-1 Regulation and Function in Nematodes.
D. Rudel and J. Kimble (2001)
Genetics 157, 639-654
   Abstract »    Full Text »
Proapoptotic p53-Interacting Protein 53BP2 Is Induced by UV Irradiation but Suppressed by p53.
C. D. Lopez, Y. Ao, L. H. Rohde, T. D. Perez, D. J. O'Connor, X. Lu, J. M. Ford, and L. Naumovski (2000)
Mol. Cell. Biol. 20, 8018-8025
   Abstract »    Full Text »
AnkB, a Periplasmic Ankyrin-Like Protein in Pseudomonas aeruginosa, Is Required for Optimal Catalase B (KatB) Activity and Resistance to Hydrogen Peroxide.
M. L. Howell, E. Alsabbagh, J.-F. Ma, U. A. Ochsner, M. G. Klotz, T. J. Beveridge, K. M. Blumenthal, E. C. Niederhoffer, R. E. Morris, D. Needham, et al. (2000)
J. Bacteriol. 182, 4545-4556
   Abstract »    Full Text »
The importance of being proline: the interaction of proline-rich motifs in signaling proteins with their cognate domains.
B. K. KAY, M. P. WILLIAMSON, and M. SUDOL (2000)
FASEB J 14, 231-241
   Abstract »    Full Text »
Spectrin tethers and mesh in the biosynthetic pathway.
M. De Matteis and J. Morrow (2000)
J. Cell Sci. 113, 2331-2343
   Abstract »    PDF »
APCL, a Central Nervous System-specific Homologue of Adenomatous Polyposis Coli Tumor Suppressor, Binds to p53-binding Protein 2 and Translocates it to the Perinucleus.
H. Nakagawa, K. Koyama, Y. Murata, M. Morito, T. Akiyama, and Y. Nakamura (2000)
Cancer Res. 60, 101-105
   Abstract »    Full Text »
A Requirement for Ankyrin Binding to Clathrin during Coated Pit Budding.
P. Michaely, A. Kamal, R. G. W. Anderson, and V. Bennett (1999)
J. Biol. Chem. 274, 35908-35913
   Abstract »    Full Text »    PDF »
Characterization of the Shank Family of Synaptic Proteins. MULTIPLE GENES, ALTERNATIVE SPLICING, AND DIFFERENTIAL EXPRESSION IN BRAIN AND DEVELOPMENT.
S. Lim, S. Naisbitt, J. Yoon, J.-I. Hwang, P.-G. Suh, M. Sheng, and E. Kim (1999)
J. Biol. Chem. 274, 29510-29518
   Abstract »    Full Text »    PDF »
Regulation of Transcription at the Saccharomyces cerevisiae Start Transition by Stb1, a Swi6-Binding Protein.
Y. Ho, M. Costanzo, L. Moore, R. Kobayashi, and B. J. Andrews (1999)
Mol. Cell. Biol. 19, 5267-5278
   Abstract »    Full Text »    PDF »
Identification of a Novel Inhibitor of Nuclear Factor-kappa B, RelA-associated Inhibitor.
J.-P. Yang, M. Hori, T. Sanda, and T. Okamoto (1999)
J. Biol. Chem. 274, 15662-15670
   Abstract »    Full Text »    PDF »
Interaction of NPR1 with basic leucine zipper protein transcription factors that bind sequences required for salicylic acid induction of the PR-1 gene.
Y. Zhang, W. Fan, M. Kinkema, X. Li, and X. Dong (1999)
PNAS 96, 6523-6528
   Abstract »    Full Text »    PDF »
The Arabidopsis ssi1 Mutation Restores Pathogenesis-Related Gene Expression in npr1 Plants and Renders Defensin Gene Expression Salicylic Acid Dependent.
J. Shah, P. Kachroo, and D. F. Klessig (1999)
PLANT CELL 11, 191-206
   Abstract »    Full Text »
Mechanisms by which Ikappa B proteins control NF-kappa B activity.
S. Simeonidis, D. Stauber, G. Chen, W. A. Hendrickson, and D. Thanos (1999)
PNAS 96, 49-54
   Abstract »    Full Text »    PDF »
Structure and Mechanism in NF-{kappa}B/I{kappa}B Signaling.
T. HUXFORD, S. MALEK, and G. GHOSH (1999)
Cold Spring Harb Symp Quant Biol 64, 533-540
   Abstract »    PDF »
Regulation of Cdc28 Cyclin-Dependent Protein Kinase Activity during the Cell Cycle of the Yeast Saccharomyces cerevisiae.
M. D. Mendenhall and A. E. Hodge (1998)
Microbiol. Mol. Biol. Rev. 62, 1191-1243
   Abstract »    Full Text »    PDF »
Stimulation of p53-mediated Transcriptional Activation by the p53-binding Proteins, 53BP1 and 53BP2.
K. Iwabuchi, B. Li, H. F. Massa, B. J. Trask, T. Date, and S. Fields (1998)
J. Biol. Chem. 273, 26061-26068
   Abstract »    Full Text »    PDF »
Ikappa Balpha Functions through Direct Contacts with the Nuclear Localization Signals and the DNA Binding Sequences of NF-kappa B.
S. Malek, T. Huxford, and G. Ghosh (1998)
J. Biol. Chem. 273, 25427-25435
   Abstract »    Full Text »    PDF »
Structure of the Ankyrin-binding Domain of alpha -Na,K-ATPase.
Z. Zhang, P. Devarajan, A. L. Dorfman, and J. S. Morrow (1998)
J. Biol. Chem. 273, 18681-18684
   Abstract »    Full Text »    PDF »
Nuclear Localization of Ikappa Balpha Is Mediated by the Second Ankyrin Repeat: the Ikappa Balpha Ankyrin Repeats Define a Novel Class of cis-Acting Nuclear Import Sequences.
S. Sachdev, A. Hoffmann, and M. Hannink (1998)
Mol. Cell. Biol. 18, 2524-2534
   Abstract »    Full Text »
The Structure of GABPalpha /beta : An ETS Domain- Ankyrin Repeat Heterodimer Bound to DNA.
A. H. Batchelor, D. E. Piper, F. C. de la Brousse, S. L. McKnight, and C. Wolberger (1998)
Science 279, 1037-1041
   Abstract »    Full Text »
A Bipartite Model of 2-5A-dependent RNase L.
B. Dong and R. H. Silverman (1997)
J. Biol. Chem. 272, 22236-22242
   Abstract »    Full Text »    PDF »
Structure and Organization of the Human Ankyrin-1 Gene. BASIS FOR COMPLEXITY OF PRE-mRNA PROCESSING.
P. G. Gallagher, W. T. Tse, A. L. Scarpa, S. E. Lux, and B. G. Forget (1997)
J. Biol. Chem. 272, 19220-19228
   Abstract »    Full Text »    PDF »
Signal perception and transduction in plant defense responses..
Y Yang, J Shah, and D F Klessig (1997)
Genes & Dev. 11, 1621-1639
   PDF »
A proline-rich motif in p53 is required for transactivation- independent growth arrest as induced by Gas1.
E. M. Ruaro, L. Collavin, G. Del Sal, R. Haffner, M. Oren, A. J. Levine, and C. Schneider (1997)
PNAS 94, 4675-4680
   Abstract »    Full Text »    PDF »
The Adapter Type Protein CMS/CD2AP Binds to the Proto-oncogenic Protein c-Cbl through a Tyrosine Phosphorylation-regulated Src Homology 3 Domain Interaction.
K. H. Kirsch, M.-M. Georgescu, T. Shishido, W. Y. Langdon, R. B. Birge, and H. Hanafusa (2001)
J. Biol. Chem. 276, 4957-4963
   Abstract »    Full Text »    PDF »
Preparation and Crystallization of Dynamic NF-kappa B{middle dot}Ikappa B Complexes.
T. Huxford, S. Malek, and G. Ghosh (2000)
J. Biol. Chem. 275, 32800-32806
   Abstract »    Full Text »    PDF »
A Deubiquitinating Enzyme UBPY Interacts with the Src Homology 3 Domain of Hrs-binding Protein via a Novel Binding Motif PX(V/I)(D/N)RXXKP.
M. Kato, K. Miyazawa, and N. Kitamura (2000)
J. Biol. Chem. 275, 37481-37487
   Abstract »    Full Text »    PDF »
Yes-associated Protein and p53-binding Protein-2 Interact through Their WW and SH3 Domains.
X. Espanel and M. Sudol (2001)
J. Biol. Chem. 276, 14514-14523
   Abstract »    Full Text »    PDF »
High Thermostability and Lack of Cooperative DNA Binding Distinguish the p63 Core Domain from the Homologous Tumor Suppressor p53.
C. Klein, G. Georges, K.-P. Kunkele, R. Huber, R. A. Engh, and S. Hansen (2001)
J. Biol. Chem. 276, 37390-37401
   Abstract »    Full Text »    PDF »
NMR Spectroscopy Reveals the Solution Dimerization Interface of p53 Core Domains Bound to Their Consensus DNA.
C. Klein, E. Planker, T. Diercks, H. Kessler, K.-P. Kunkele, K. Lang, S. Hansen, and M. Schwaiger (2001)
J. Biol. Chem. 276, 49020-49027
   Abstract »    Full Text »    PDF »
A peptide that binds and stabilizes p53 core domain: Chaperone strategy for rescue of oncogenic mutants.
A. Friedler, L. O. Hansson, D. B. Veprintsev, S. M. V. Freund, T. M. Rippin, P. V. Nikolova, M. R. Proctor, S. Rudiger, and A. R. Fersht (2002)
PNAS 99, 937-942
   Abstract »    Full Text »    PDF »
CLIPR-59, a new trans-Golgi/TGN cytoplasmic linker protein belonging to the CLIP-170 family.
F. Perez, K. Pernet-Gallay, C. Nizak, H. V. Goodson, T. E. Kreis, and B. Goud (2002)
J. Cell Biol. 156, 631-642
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)