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Science 25 October 1996:
Vol. 274. no. 5287, pp. 614 - 617
DOI: 10.1126/science.274.5287.614
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Reports

An Asymmetric Model for the Nucleosome: A Binding Site for Linker Histones Inside the DNA Gyres

Dmitry Pruss, Blaine Bartholomew, Jim Persinger, Jeffrey Hayes, Gina Arents, Evangelos N. Moudrianakis, Alan P. Wolffe *

Histone-DNA contacts within a nucleosome influence the function of trans-acting factors and the molecular machines required to activate the transcription process. The internal architecture of a positioned nucleosome has now been probed with the use of photoactivatable cross-linking reagents to determine the placement of histones along the DNA molecule. A model for the nucleosome is proposed in which the winged-helix domain of the linker histone is asymmetrically located inside the gyres of DNA that also wrap around the core histones. This domain extends the path of the protein superhelix to one side of the core particle.

D. Pruss and A. P. Wolffe, Laboratory of Molecular Embryology, National Institute of Child Health and Human Development, National Institutes of Health, Building 6, Room B1A-13, Bethesda, MD 20892-2710, USA.
B. Bartholomew and J. Persinger, Department of Medical Biochemistry, Southern Illinois University at Carbondale, School of Medicine, Carbondale, IL 62901-4413, USA.
J. Hayes, Department of Biochemistry, School of Medicine and Dentistry, University of Rochester, Rochester, NY 14642, USA.
G. Arents and E. N. Moudrianakis, Department of Biology, Johns Hopkins University, Baltimore, MD 21218, USA.
*   To whom correspondence should be addressed. E-mail: awlme{at}helix.nih.gov

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