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Science 25 October 1996:
Vol. 274. no. 5287, pp. 602 - 605
DOI: 10.1126/science.274.5287.602
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Reports

Evidence for Cell-Surface Association Between Fusin and the CD4-gp120 Complex in Human Cell Lines

Cheryl K. Lapham, * Jun Ouyang, Bhaskar Chandrasekhar, Nga Y. Nguyen, Dimiter S. Dimitrov, Hana Golding

Accessory cell-surface molecules involved in the entry of human immunodeficiency virus-type 1 into cells have recently been identified and shown to belong to the family of chemokine receptors. Treatment of human cell lines with soluble monomeric gp120 at 37°C induced an association between the surface CD4-gp120 complex and a 45-kilodalton protein, which can be down-modulated by the phorbol ester phorbol 12-myristate 13-acetate. The three proteins were coprecipitated from the cell membranes with antibodies to CD4 or to gp120. The 45-kilodalton protein comigrated with fusin on sodium dodecyl sulfate gels and reacted with rabbit antisera to fusin in protein immunoblots. No 45-kilodalton protein could be coprecipitated from similarly treated nonhuman cells. However, infection of 3T3.CD4.401 cells with vaccinia-fusin recombinant virus (vCBYF1), followed by gp120 treatment, resulted in coprecipitation of fusin and CD4.401 molecules from their membranes. Together these data provide evidence for physical association between fusin and the CD4-gp120 complex on cell membranes.

C. K. Lapham and H. Golding, Division of Viral Products, Center for Biologics Evaluation and Research (CBER), Food and Drug Administration, Building 29B, Room 3G21, HFM 454, 8800 Rockville Pike, Bethesda, MD 20892, USA.
J. Ouyang and D. S. Dimitrov, Section on Membrane Structure and Function, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
B. Chandrasekhar and N. Y. Nguyen, Facility for Biotechnology Resources, CBER, Food and Drug Administration, Bethesda, MD 20892, USA.
*   To whom correspondence should be addressed.

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   Abstract »    Full Text »    PDF »
Identification of V3 Loop-binding Proteins as Potential Receptors Implicated in the Binding of HIV Particles to CD4+ Cells.
C. Callebaut, J. Blanco, N. Benkirane, B. Krust, E. Jacotot, G. Guichard, N. Seddiki, J. Svab, E. Dam, S. Muller, et al. (1998)
J. Biol. Chem. 273, 21988-21997
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The leukotriene B4 receptor functions as a novel type of coreceptor mediating entry of primary HIV-1 isolates into CD4-positive cells.
C. Owman, A. Garzino-Demo, F. Cocchi, M. Popovic, A. Sabirsh, and R. C. Gallo (1998)
PNAS 95, 9530-9534
   Abstract »    Full Text »    PDF »
The Cytomegalovirus-Encoded Chemokine Receptor US28 Can Enhance Cell-Cell Fusion Mediated by Different Viral Proteins.
O. Pleskoff, C. Treboute, and M. Alizon (1998)
J. Virol. 72, 6389-6397
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gp120 envelope glycoproteins of human immunodeficiency viruses competitively antagonize signaling by coreceptors CXCR4 and CCR5.
N. Madani, S. L. Kozak, M. P. Kavanaugh, and D. Kabat (1998)
PNAS 95, 8005-8010
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CXCR4 Sequences Involved in Coreceptor Determination of Human Immunodeficiency Virus Type-1 Tropism. UNMASKING OF ACTIVITY WITH M-TROPIC Env GLYCOPROTEINS.
Z.-x. Wang, J. F. Berson, T.-y. Zhang, Y.-H. Cen, Y. Sun, M. Sharron, Z.-h. Lu, and S. C. Peiper (1998)
J. Biol. Chem. 273, 15007-15015
   Abstract »    Full Text »    PDF »
Similar Levels of Human Immunodeficiency Virus Type 1 Replication in Human TH1 and TH2 Clones.
J. A. Mikovits, D. D. Taub, S. M. Turcovski-Corrales, and F. W. Ruscetti (1998)
J. Virol. 72, 5231-5238
   Abstract »    Full Text »    PDF »
Human Immunodeficiency Virus Type 1 Attachment to HeLa CD4 Cells Is CD4 Independent and gp120 Dependent and Requires Cell Surface Heparans.
I. Mondor, S. Ugolini, and Q. J. Sattentau (1998)
J. Virol. 72, 3623-3634
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T-Cell-Line-Tropic Human Immunodeficiency Virus Type 1 That Is Made Resistant to Stromal Cell-Derived Factor 1alpha Contains Mutations in the Envelope gp120 but Does Not Show a Switch in Coreceptor Use.
D. Schols, J. A. Este, C. Cabrera, and E. De Clercq (1998)
J. Virol. 72, 4032-4037
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Specific Interaction of HIV-1 and HIV-2 Surface Envelope Glycoproteins with Monolayers of Galactosylceramide and Ganglioside GM3.
D. Hammache, G. Pieroni, N. Yahi, O. Delezay, N. Koch, H. Lafont, C. Tamalet, and J. Fantini (1998)
J. Biol. Chem. 273, 7967-7971
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Effects of CCR5 and CD4 Cell Surface Concentrations on Infections by Macrophagetropic Isolates of Human Immunodeficiency Virus Type 1.
E. J. Platt, K. Wehrly, S. E. Kuhmann, B. Chesebro, and D. Kabat (1998)
J. Virol. 72, 2855-2864
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Multiple Residues Contribute to the Inability of Murine CCR-5 To Function as a Coreceptor for Macrophage-Tropic Human Immunodeficiency Virus Type 1 Isolates.
T. M. Ross, P. D. Bieniasz, and B. R. Cullen (1998)
J. Virol. 72, 1918-1924
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Identification of a Domain within the Human T-Cell Leukemia Virus Type 2 Envelope Required for Syncytium Induction and Replication.
B. Poon and I. S. Y. Chen (1998)
J. Virol. 72, 1959-1966
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Modulation of Feline Immunodeficiency Virus Infection by Stromal Cell-Derived Factor.
M. J. Hosie, N. Broere, J. Hesselgesser, J. D. Turner, J. A. Hoxie, J. C. Neil, and B. J. Willett (1998)
J. Virol. 72, 2097-2104
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T Cells Expressing Activated LFA-1 Are More Susceptible to Infection with Human Immunodeficiency Virus Type 1 Particles Bearing Host-Encoded ICAM-1.
J.-F. Fortin, R. Cantin, and M. J. Tremblay (1998)
J. Virol. 72, 2105-2112
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Cytosolic Gag p24 as an Index of Productive Entry of Human Immunodeficiency Virus Type 1.
V. Marechal, F. Clavel, J. M. Heard, and O. Schwartz (1998)
J. Virol. 72, 2208-2212
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Human Immunodeficiency Virus (HIV) Envelope Binds to CXCR4 Independently of CD4, and Binding Can Be Enhanced by Interaction with Soluble CD4 or by HIV Envelope Deglycosylation.
J. C. Bandres, Q. F. Wang, J. O'Leary, F. Baleaux, A. Amara, J. A. Hoxie, S. Zolla-Pazner, and M. K. Gorny (1998)
J. Virol. 72, 2500-2504
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Involvement of Extracellular Signal-Regulated Kinase Module in HIV-Mediated CD4 Signals Controlling Activation of Nuclear Factor-{kappa}B and AP-1 Transcription Factors.
L. Briant, V. Robert-Hebmann, V. Sivan, A. Brunet, J. Pouyssegur, and C. Devaux (1998)
J. Immunol. 160, 1875-1885
   Abstract »    Full Text »    PDF »
Chemokines -- Chemotactic Cytokines That Mediate Inflammation.
A. D. Luster (1998)
N. Engl. J. Med. 338, 436-445
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Induction of Neutralizing Antibodies to T-Cell Line-Adapted and Primary Human Immunodeficiency Virus Type 1 Isolates with a Prime-Boost Vaccine Regimen in Chimpanzees.
S. Zolla-Pazner, M. Lubeck, S. Xu, S. Burda, R. J. Natuk, F. Sinangil, K. Steimer, R. C. Gallo, J. W. Eichberg, T. Matthews, et al. (1998)
J. Virol. 72, 1052-1059
   Abstract »    Full Text »    PDF »
Use of Murine CXCR-4 as a Second Receptor by Some T-Cell-Tropic Human Immunodeficiency Viruses.
C. Parolin, A. Borsetti, H. Choe, M. Farzan, P. Kolchinsky, M. Heesen, Q. Ma, C. Gerard, G. Palu, M. E. Dorf, et al. (1998)
J. Virol. 72, 1652-1656
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Intracellular and Surface Expression of the HIV-1 Coreceptor CXCR4/Fusin on Various Leukocyte Subsets: Rapid Internalization and Recycling Upon Activation.
R. Forster, E. Kremmer, A. Schubel, D. Breitfeld, A. Kleinschmidt, C. Nerl, G. Bernhardt, and M. Lipp (1998)
J. Immunol. 160, 1522-1531
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Identification and Characterization of the CXCR4 Chemokine Receptor in Human T Cell Lines: Ligand Binding, Biological Activity, and HIV-1 Infectivity.
J. Hesselgesser, M. Liang, J. Hoxie, M. Greenberg, L. F. Brass, M. J. Orsini, D. Taub, and R. Horuk (1998)
J. Immunol. 160, 877-883
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Differential regulation of CXCR4 and CCR5 endocytosis.
N Signoret, M. Rosenkilde, P. Klasse, T. Schwartz, M. Malim, J. Hoxie, and M Marsh (1998)
J. Cell Sci. 111, 2819-2830
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Conformational Changes in Cell Surface HIV-1 Envelope Glycoproteins Are Triggered by Cooperation between Cell Surface CD4 and Co-receptors.
P. L. St. J. Jones, T. Korte, and R. Blumenthal (1998)
J. Biol. Chem. 273, 404-409
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Detection and Delineation of CXCR-4 (Fusin) as an Entry and Fusion Cofactor for T Cell-Tropic HIV-1 by Three Different Monoclonal Antibodies.
T. Hori, H. Sakaida, A. Sato, T. Nakajima, H. Shida, O. Yoshie, and T. Uchiyama (1998)
J. Immunol. 160, 180-188
   Abstract »    Full Text »    PDF »
In Vitro Behavior of Hematopoietic Progenitor Cells Under the Influence of Chemoattractants: Stromal Cell-Derived Factor-1, Steel Factor, and the Bone Marrow Environment.
C. H. Kim and H. E. Broxmeyer (1998)
Blood 91, 100-110
   Abstract »    Full Text »    PDF »
CD4-Independent Binding of SIV gp120 to Rhesus CCR5.
K. A. Martin, R. Wyatt, M. Farzan, H. Choe, L. Marcon, E. Desjardins, J. Robinson, J. Sodroski, C. Gerard, and N. P. Gerard (1997)
Science 278, 1470-1473
   Abstract »    Full Text »


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