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Science 20 September 1996:
Vol. 273. no. 5282, pp. 1702 - 1705
DOI: 10.1126/science.273.5282.1702
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Reports

Parallel and Antiparallel (G·GC)2 Triple Helix Fragments in a Crystal Structure

Dominique Vlieghe, Luc Van Meervelt, Alain Dautant, Bernard Gallois, Gilles Précigoux, Olga Kennard *

Nucleic acid triplexes are formed by sequence-specific interactions between single-stranded polynucleotides and the double helix. These triplexes are implicated in genetic recombination in vivo and have application to areas that include genome analysis and antigene therapy. Despite the importance of the triple helix, only limited high-resolution structural information is available. The x-ray crystal structure of the oligonucleotide d(GGCCAATTGG) is described; it was designed to contain the d(G·GC)2 fragment and thus provide the basic repeat unit of a DNA triple helix. Parameters derived from this crystal structure have made it possible to construct models of both parallel and antiparallel triple helices.

D. Vlieghe and L. Van Meervelt, Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, B-3001 Heverlee, Belgium.
A. Dautant, B. Gallois, G. Précigoux, Unité de Biophysique Structurale, EP CNRS, Université de Bordeaux II, 33405 Talence, France.
O. Kennard, Cambridge Crystallographic Data Centre, 12 Union Road, Cambridge CB2 1EZ, UK.
*   To whom correspondence should be addressed.

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