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Science 30 August 1996:
Vol. 273. no. 5279, pp. 1231 - 1233
DOI: 10.1126/science.273.5279.1231
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Reports

Modulation of Virulence Factor Expression by Pathogen Target Cell Contact

Jonas Pettersson, * Roland Nordfelth, * Elena Dubinina, Tomas Bergman, Mikael Gustafsson, Karl Eric Magnusson, Hans Wolf-Watz dagger

Upon contact with the eukaryotic cell, Yersinia pseudotuberculosis increased the rate of transcription of virulence genes (yop), as determined by in situ monitoring of light emission from individual bacteria expressing luciferase under the control of the yopE promoter. The microbe-host interaction triggered export of LcrQ, a negative regulator of Yop expression, via the Yop-type III secretion system. The intracellular concentration of LcrQ was thereby lowered, resulting in increased expression of Yops. These results suggest a key role for the type III secretion system of pathogenic bacteria to coordinate secretion with expression of virulence factors after physical contact with the target cell.

J. Pettersson, R. Nordfelth, E. Dubinina, T. Berman, H. Wolf-Watz, Department of Cell and Molecular Biology, University of Umeå, S-901 87 Umeå, Sweden.
M. Gustafsson and K. E. Magnusson, Department of Medical Microbiology, University of Linköping, S-581 85 Linköping, Sweden.
*   These authors have contributed equally to this work.

dagger    To whom correspondence should be addressed. E-mail: hans.wolf-watz{at}cmb.umu.se


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