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Science 2 August 1996:
Vol. 273. no. 5275, pp. 660 - 663
DOI: 10.1126/science.273.5275.660
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Reports

Function of Myosin-V in Filopodial Extension of Neuronal Growth Cones

Feng-Song Wang, Joseph S. Wolenski, Richard E. Cheney, * Mark S. Mooseker, Daniel G. Jay dagger

The molecular mechanisms underlying directed motility of growth cones have not been determined. The role of myosin-V, an unconventional myosin, in growth cone dynamics was examined by chromophore-assisted laser inactivation (CALI). CALI of purified chick brain myosin-V absorbed onto nitrocellulose-coated cover slips inhibited the ability of myosin-V to translocate actin filaments. CALI of myosin-V in growth cones of chick dorsal root ganglion neurons resulted in rapid filopodial retraction. The rate of filopodial extension was significantly decreased, whereas the rate of filopodial retraction was not affected, which suggests a specific role for myosin-V in filopodial extension.

F.-S. Wang and D. G. Jay, Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA.
J. S. Wolenski, R. E. Cheney, M. S. Mooseker, Department of Biology, Yale University, New Haven, CT 06520, USA.
*   Present address: Department of Physiology, University of North Carolina, Chapel Hill, NC 27599, USA.

dagger    To whom correspondence should be addressed. E-mail: jay{at}biosun.harvard.edu


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