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Science 19 April 1996:
Vol. 272. no. 5260, pp. 419 - 421
DOI: 10.1126/science.272.5260.419
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Reports

Circadian Rhythms in Cultured Mammalian Retina

Gianluca Tosini and Michael Menaker *

Many retinal functions are circadian, but in most instances the location of the clock that drives the rhythm is not known. Cultured neural retinas of the golden hamster (Mesocricetus auratus) exhibited circadian rhythms of melatonin synthesis for at least 5 days at 27°C. The rhythms were entrained by light cycles applied in vitro and were free-running in constant darkness. Retinas from hamsters homozygous for the circadian mutation tau, which shortens the free-running period of the circadian activity rhythm by 4 hours, showed a shortened free-running period of melatonin synthesis. The mammalian retina contains a genetically programmed circadian oscillator that regulates its synthesis of melatonin.

Department of Biology and National Science Foundation Center for Biological Timing, University of Virginia, Charlottesville, VA 22903, USA.
* To whom correspondence should be addressed.


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