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Science 23 February 1996:
Vol. 271. no. 5252, pp. 1123 - 1125
DOI: 10.1126/science.271.5252.1123
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Reports

Locus Control Region Function and Heterochromatin-Induced Position Effect Variegation

Richard Festenstein, (1) Mauro Tolaini, Paola Corbella, Clio Mamalaki, Jenny Parrington, Margaret Fox, Antigoni Miliou, Margaret Jones, Dimitris Kioussis (1)

Human CD2 locus control region (LCR) sequences are shown here to be essential for establishing an open chromatin configuration. Transgenic mice carrying an hCD2 minigene attached only to the 3' CD2 transcriptional enhancer exhibited variegated expression when the transgene integrated in the centromere. In contrast, mice carrying a transgene with additional 3' sequences showed no variegation even when the latter integrated in centromeric positions. This result suggests that LCRs operate by ensuring an open chromatin configuration and that a short region, with no enhancer activity, functions in the establishment, maintenance, or both of an open chromatin domain.

R. Festenstein, M. Tolaini, P. Corbella, C. Mamalaki, D. Kioussis, Division of Molecular Immunology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK.
J. Parrington, M. Fox, A. Miliou, Medical Research Council Human Biochemical Genetics Unit, Galton Laboratory, University College London, Wolfson House, 4 Stephenson Way, London NW1 2HE, UK.
M. Jones, Nuffield Department of Pathologgy, John Radcliffe Hospital, Oxford OX3 9DU, UK.
(1) To whom correspondence should be addressed.


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