Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 2 February 1996:
Vol. 271. no. 5249, pp. 662 - 665
DOI: 10.1126/science.271.5249.662
Prev | Table of Contents | Next

Reports

Expression of a Plasmodium Gene Introduced into Subtelomeric Regions of Plasmodium berghei Chromosomes

Melissa R. van Dijk,  Chris J. Janse,  Andrew P. Waters (1)

Targeted integration of exogenous DNA into the genome of malaria parasites will allow their phenotype to be modulated by means of gene disruption or the stable expression of foreign and mutated genes. Described here is the site-specific integration through reciprocal exchange, and subsequent expression, of a selectable marker gene into the genome of the pathogenic, bloodstage forms of the rodent malaria parasite Plasmodium berghei. Stable integration of a single copy of the marker gene (retained for more than 70 generations in the absence of drug pressure) into a nontranscribed subtelomeric repeat array of different chromosomes was observed. Expression of the gene within the subtelomeres indicated that the previously recorded absence of transcription in these regions could be due to a corresponding absence of genes rather than active silencing mechanisms.


Department of Parasitology, University of Leiden, 2300 AL Leiden, Netherlands.
(1) To whom correspondence should be addressed.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Conditional mutagenesis using site-specific recombination in Plasmodium berghei.
T. G. Carvalho, S. Thiberge, H. Sakamoto, and R. Menard (2004)
PNAS 101, 14931-14936
   Abstract »    Full Text »    PDF »
Plasmodium knowlesi Provides a Rapid In Vitro and In Vivo Transfection System That Enables Double-Crossover Gene Knockout Studies.
C. H. M. Kocken, H. Ozwara, A. van der Wel, A. L. Beetsma, J. M. Mwenda, and A. W. Thomas (2002)
Infect. Immun. 70, 655-660
   Abstract »    Full Text »    PDF »
An alteration in concatameric structure is associated with efficient segregation of plasmids in transfected Plasmodium falciparum parasites.
R. A. O'Donnell, P. R. Preiser, D. H. Williamson, P. W. Moore, A. F. Cowman, and B. S. Crabb (2001)
Nucleic Acids Res. 29, 716-724
   Abstract »    Full Text »    PDF »
Targeted Terminal Deletions as a Tool for Functional Genomics Studies in Plasmodium.
T. Pace, R. Scotti, C. J. Janse, A. P. Waters, C. Birago, and M. Ponzi (2000)
Genome Res. 10, 1414-1420
   Abstract »    Full Text »
Green Fluorescent Protein as a Marker in Plasmodium berghei Transformation.
A. A. Sultan, V. Thathy, V. Nussenzweig, and R. Menard (1999)
Infect. Immun. 67, 2602-2606
   Abstract »    Full Text »    PDF »
Subtle Mutagenesis by Ends-in Recombination in Malaria Parasites.
A. Nunes, V. Thathy, T. Bruderer, A. A. Sultan, R. S. Nussenzweig, and R. Menard (1999)
Mol. Cell. Biol. 19, 2895-2902
   Abstract »    Full Text »    PDF »
Precise Timing of Expression of a Plasmodium falciparum-derived Transgene in Plasmodium berghei Is a Critical Determinant of Subsequent Subcellular Localization.
C. H. M. Kocken, A. M. van der Wel, M. A. Dubbeld, D. L. Narum, F. M. van de Rijke, G.-J. van Gemert, X. van der Linde, L. H. Bannister, C. Janse, A. P. Waters, et al. (1998)
J. Biol. Chem. 273, 15119-15124
   Abstract »    Full Text »    PDF »
Transfection of the Primate Malaria Parasite Plasmodium knowlesi Using Entirely Heterologous Constructs.
A. M. v. d. Wel, A. M. Tomas, C. H.M. Kocken, P. Malhotra, C. J. Janse, A. P. Waters, and A. W. Thomas (1997)
J. Exp. Med. 185, 1499-1504
   Abstract »    Full Text »    PDF »
Antifolate-resistant mutants of Plasmodium falciparum dihydrofolate reductase.
W. Sirawaraporn, T. Sathitkul, R. Sirawaraporn, Y. Yuthavong, and D. V. Santi (1997)
PNAS 94, 1124-1129
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)