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Science 22 December 1995:
Vol. 270. no. 5244, pp. 1992 - 1994
DOI: 10.1126/science.270.5244.1992
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Reports

Tissue Plasminogen Activator Induction in Purkinje Neurons After Cerebellar Motor Learning

Nicholas W. Seeds (1),  Brian L. Williams,  Paula C. Bickford

The cerebellar cortex is implicated in the learning of complex motor skills. This learning may require synaptic remodeling of Purkinje cell inputs. An extracellular serine protease, tissue plasminogen activator (tPA), is involved in remodeling various nonneural tissues and is associated with developing and regenerating neurons. In situ hybridization showed that expression of tPA messenger RNA was increased in the Purkinje neurons of rats within an hour of their being trained for a complex motor task. Antibody to tPA also showed the induction of tPA protein associated with cerebellar Purkinje cells. Thus, the induction of tPA during motor learning may play a role in activity-dependent synaptic plasticity.


N. W. Seeds, Neuroscience Program and Department of Biochemistry/Biophysics and Genetics, University of Colorado Health Sciences Center, Denver, CO 80262, USA.
B. L. Williams, Medical Scientist Training Program, University of Colorado Health Sciences Center, Denver, CO 80262, USA.
P. C. Bickford, Department of Pharmacology and Veterans Administration Medical Center, University of Colorado Health Sciences Center, Denver, CO 80262, USA.
(1) To whom correspondence should be addressed.


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