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Science 15 December 1995:
Vol. 270. no. 5243, pp. 1831 - 1835
DOI: 10.1126/science.270.5243.1831
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Reports

Precise Spatial Positioning of Chromosomes During Prometaphase: Evidence for Chromosomal Order

Robert Nagele (1) (2),  Theresa Freeman (1),  Lydia McMorrow,  Hsin-yi Lee

The relative locations of several chromosomes within wheel-shaped prometaphase chromosome rosettes of human fibroblasts and HeLa cells were determined with fluorescence hybridization. Homologs were consistently positioned on opposite sides of the rosette, which suggests that chromosomes are separated into two haploid sets, each derived from one parent. The relative locations of chromosomes on the rosette were mapped by dual hybridizations. The data suggest that the chromosome orders within the two haploid sets are antiparallel. This chromosome arrangement in human cells appears to be both independent of cell type- and species-specific and may influence chromosome topology throughout the cell cycle.


R. Nagele and T. Freeman, Department of Molecular Biology, University of Medicine and Dentistry of New Jersey, School of Osteopathic Medicine, Stratford, NJ 08084, USA.
L. McMorrow, Department of Laboratory Science, Thomas Jefferson University, Philadelphia, PA 19107, USA.
H.-y. Lee, Department of Biology, Rutgers University, Camden, NJ 08102, USA.
(1) These authors contributed equally to this work.
(2) To whom correspondence should be addressed.


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