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Science 8 December 1995:
Vol. 270. no. 5242, pp. 1660 - 1663
DOI: 10.1126/science.270.5242.1660
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Reports

Sodium-Driven Potassium Uptake by the Plant Potassium Transporter HKT1 and Mutations Conferring Salt Tolerance

Francisco Rubio,  Walter Gassmann,  Julian I. Schroeder

Sodium (Na+) at high millimolar concentrations in soils is toxic to most higher plants and severely reduces agricultural production worldwide. However, the molecular mechanisms for plant Na+ uptake remain unknown. Here, the wheat root high-affinity potassium (K+) uptake transporter HKT1 was shown to function as a high-affinity K+-Na+ cotransporter. High-affinity K+ uptake was activated by micromolar Na+ concentrations; moreover, high-affinity Na+ uptake was activated by K+ (half-activation constant, 2.8 µM K+). However, at physiologically detrimental concentrations of Na+, K+ accumulation mediated by HKT1 was blocked and low-affinity Na+ uptake occurred (Michaelis constant, 16 mM Na+), which correlated to Na+ toxicity in plants. Point mutations in the sixth putative transmembrane domain of HKT1 that increase Na+ tolerance were isolated with the use of yeast as a screening system. Na+ uptake and Na+ inhibition of K+ accumulation indicate a possible role for HKT1 in physiological Na+ toxicity in plants.


Department of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla, CA 92093-0116, USA.


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The Arabidopsis thaliana SOS2 gene encodes a protein kinase that is required for salt tolerance.
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Altered selectivity in an Arabidopsis metal transporter.
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PLANT CELL 14, 575-588
   Abstract »    Full Text »    PDF »


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