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Science 3 November 1995:
Vol. 270. no. 5237, pp. 805 - 808
DOI: 10.1126/science.270.5237.805
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Reports

Positional Cloning and Sequence Analysis of the Drosophila Clock Gene, timeless

Michael P. Myers,  Karen Wager-Smith,  Cedric S. Wesley,  Michael W. Young (1),  Amita Sehgal

The Drosophila genes timeless (tim) and period (per) interact, and both are required for production of circadian rhythms. Here the positional cloning and sequencing of tim are reported. The tim gene encodes a previously uncharacterized protein of 1389 amino acids, and possibly another protein of 1122 amino acids. The arrhythmic mutation tim is a 64-base pair deletion that truncates TIM to 749 amino acids. Absence of sequence similarity to the PER dimerization motif (PAS) indicates that direct interaction between PER and TIM would require a heterotypic protein association.


M. P. Myers, K. Wager-Smith, C. S. Wesley, M. W. Young, Howard Hughes Medical Institute, National Science Foundation Science and Technology Center for Biological Timing, and Laboratory of Genetics, Rockefeller University, New York, NY 10021, USA.
A. Sehgal, Department of Neuroscience and Center for Sleep and Respiratory Neurobiology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.
(1) To whom correspondence should be addressed.


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