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Science 29 September 1995:
Vol. 269. no. 5232, pp. 1866 - 1869
DOI: 10.1126/science.7569926
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Articles

Science, Vol 269, Issue 5232, 1866-1869
Copyright © 1995 by American Association for the Advancement of Science

articles

Modulation of transcription factor Ets-1 DNA binding: DNA-induced unfolding of an alpha helix

JM Petersen, JJ Skalicky, LW Donaldson, LP McIntosh, T Alber, and BJ Graves

Department of Oncological Sciences, University of Utah School of Medicine, Salt Lake City 84132, USA.

Conformational changes, including local protein folding, play important roles in protein-DNA interactions. Here, studies of the transcription factor Ets-1 provided evidence that local protein unfolding also can accompany DNA binding. Circular dichroism and partial proteolysis showed that the secondary structure of the Ets-1 DNA-binding domain is unchanged in the presence of DNA. In contrast, DNA allosterically induced the unfolding of an alpha helix that lies within a flanking region involved in the negative regulation of DNA binding. These findings suggest a structural basis for the intramolecular inhibition of DNA binding and a mechanism for the cooperative partnerships that are common features of many eukaryotic transcription factors.


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Pip, a lymphoid-restricted IRF, contains a regulatory domain that is important for autoinhibition and ternary complex formation with the Ets factor PU.1..
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A Novel Family of Developmentally Regulated Mammalian Transcription Factors Containing the TEA/ATTS DNA Binding Domain.
P. Jacquemin, J.-J. Hwang, J. A. Martial, P. Dolle, and I. Davidson (1996)
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Pax-5 (BSAP) recruits Ets proto-oncogene family proteins to form functional ternary complexes on a B-cell-specific promoter..
D Fitzsimmons, W Hodsdon, W Wheat, S M Maira, B Wasylyk, and J Hagman (1996)
Genes & Dev. 10, 2198-2211
   Abstract »    PDF »
The product of the mouse nude locus, Whn, regulates the balance between epithelial cell growth and differentiation..
J L Brissette, J Li, J Kamimura, D Lee, and G P Dotto (1996)
Genes & Dev. 10, 2212-2221
   Abstract »    PDF »
The EVES motif mediates both intermolecular and intramolecular regulation of c-Myb..
A B Dash, F C Orrico, and S A Ness (1996)
Genes & Dev. 10, 1858-1869
   Abstract »    PDF »
p53: puzzle and paradigm..
L J Ko and C Prives (1996)
Genes & Dev. 10, 1054-1072
   PDF »
Transcription revisited: a commentary on the 1995 Cold Spring Harbor Laboratory meeting, "Mechanisms of Eukaryotic Transcription"..
S L McKnight (1996)
Genes & Dev. 10, 367-381
   PDF »
MRG1 Expression in Fibroblasts Is Regulated by Sp1/Sp3 and an Ets Transcription Factor.
B. Han, N. Liu, X. Yang, H. B. Sun, and Y.-C. Yang (2001)
J. Biol. Chem. 276, 7937-7942
   Abstract »    Full Text »    PDF »
PU.1-mediated Transcription Is Enhanced by HMG-I(Y)-dependent Structural Mechanisms.
R. T. Lewis, A. Andreucci, and B. S. Nikolajczyk (2001)
J. Biol. Chem. 276, 9550-9557
   Abstract »    Full Text »    PDF »
DNA Binding by the ETS-domain Transcription Factor PEA3 Is Regulated by Intramolecular and Intermolecular Protein{middle dot}Protein Interactions.
A. Greenall, N. Willingham, E. Cheung, D. S. Boam, and A. D. Sharrocks (2001)
J. Biol. Chem. 276, 16207-16215
   Abstract »    Full Text »    PDF »
Physical Interaction and Functional Synergy between Glucocorticoid Receptor and Ets2 Proteins for Transcription Activation of the Rat Cytochrome P-450c27 Promoter.
J. Mullick, H. K. Anandatheerthavarada, G. Amuthan, S. V. Bhagwat, G. Biswas, V. Camasamudram, N. K. Bhat, S. E. P. Reddy, V. Rao, and N. G. Avadhani (2001)
J. Biol. Chem. 276, 18007-18017
   Abstract »    Full Text »    PDF »
ERM Transactivation Is Up-regulated by the Repression of DNA Binding after the PKA Phosphorylation of a Consensus Site at the Edge of the ETS Domain.
J.-L. Baert, C. Beaudoin, L. Coutte, and Y. de Launoit (2002)
J. Biol. Chem. 277, 1002-1012
   Abstract »    Full Text »    PDF »
Inhibitory Module of Ets-1 Allosterically Regulates DNA Binding through a Dipole-facilitated Phosphate Contact.
H. Wang, L. P. McIntosh, and B. J. Graves (2002)
J. Biol. Chem. 277, 2225-2233
   Abstract »    Full Text »    PDF »


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