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Science 8 September 1995:
Vol. 269. no. 5229, pp. 1429 - 1431
DOI: 10.1126/science.7660126
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Articles

Science, Vol 269, Issue 5229, 1429-1431
Copyright © 1995 by American Association for the Advancement of Science

articles

The chromodomain protein Swi6: a key component at fission yeast centromeres

K Ekwall, JP Javerzat, A Lorentz, H Schmidt, G Cranston, and R Allshire

Medical Research Council Human Genetics Unit, Western General Hospital, Edinburgh, Scotland, UK.

Centromeres attach chromosomes to the spindle during mitosis, thereby ensuring the equal distribution of chromosomes into daughter cells. Transcriptionally silent heterochromatin of unknown function is associated with centromeres in many organisms. In the fission yeast Schizosaccharomyces pombe, the silent mating-type loci, centromeres, and telomeres are assembled into silent heterochromatin-like domains. The Swi6 chromodomain protein affects this silencing, and now it is shown that Swi6p localizes with these three chromosomal regions. In cells lacking Swi6p, centromeres lag on the spindle during anaphase and chromosomes are lost at high rates. Thus, Swi6p is located at fission yeast centromeres and is required for their proper function.


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P. A. Wigge and J. V. Kilmartin (2001)
J. Cell Biol. 152, 349-360
   Abstract »    Full Text »    PDF »
Conservation of Heterochromatin Protein 1 Function.
G. Wang, A. Ma, C.-m. Chow, D. Horsley, N. R. Brown, I. G. Cowell, and P. B. Singh (2000)
Mol. Cell. Biol. 20, 6970-6983
   Abstract »    Full Text »
Four Chromo-domain Proteins of Schizosaccharomyces pombe Differentially Repress Transcription at Various Chromosomal Locations.
G. Thon and J. Verhein-Hansen (2000)
Genetics 155, 551-568
   Abstract »    Full Text »
Fission yeast Hrp1, a chromodomain ATPase, is required for proper chromosome segregation and its overexpression interferes with chromatin condensation.
E. J. Yoo, Y. H. Jin, Y. K. Jang, P. Bjerling, M. Tabish, S. H. Hong, K. Ekwall, and S. D. Park (2000)
Nucleic Acids Res. 28, 2004-2011
   Abstract »    Full Text »    PDF »
Distinct protein interaction domains and protein spreading in a complex centromere.
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Cell cycle-dependent phosphorylation of the ATRX protein correlates with changes in nuclear matrix and chromatin association.
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Hum. Mol. Genet. 9, 539-547
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Live analysis of lagging chromosomes during anaphase and their effect on spindle elongation rate in fission yeast.
A. Pidoux, S Uzawa, P. Perry, W. Cande, and R. Allshire (2000)
J. Cell Sci. 113, 4177-4191
   Abstract »    PDF »
Fission Yeast Mutants That Alleviate Transcriptional Silencing in Centromeric Flanking Repeats and Disrupt Chromosome Segregation.
K. Ekwall, G. Cranston, and R. C. Allshire (1999)
Genetics 153, 1153-1169
   Abstract »    Full Text »
The Schizosaccharomyces pombe hst4+ Gene Is a SIR2 Homologue with Silencing and Centromeric Functions.
L. L. Freeman-Cook, J. M. Sherman, C. B. Brachmann, R. C. Allshire, J. D. Boeke, and L. Pillus (1999)
Mol. Biol. Cell 10, 3171-3186
   Abstract »    Full Text »
Defects in Components of the Proteasome Enhance Transcriptional Silencing at Fission Yeast Centromeres and Impair Chromosome Segregation.
J.-P. Javerzat, G. McGurk, G. Cranston, C. Barreau, P. Bernard, C. Gordon, and R. Allshire (1999)
Mol. Cell. Biol. 19, 5155-5165
   Abstract »    Full Text »    PDF »
Proper metaphase spindle length is determined by centromere proteins Mis12 and Mis6 required for faithful chromosome segregation.
G. Goshima, S. Saitoh, and M. Yanagida (1999)
Genes & Dev. 13, 1664-1677
   Abstract »    Full Text »
Transcriptional Repression by XPc1, a New Polycomb Homolog in Xenopus laevis Embryos, Is Independent of Histone Deacetylase.
J. Strouboulis, S. Damjanovski, D. Vermaak, F. Meric, and A. P. Wolffe (1999)
Mol. Cell. Biol. 19, 3958-3968
   Abstract »    Full Text »    PDF »
Position Effect Variegation at the Mating-Type Locus of Fission Yeast: A cis-Acting Element Inhibits Covariegated Expression of Genes in the Silent and Expressed Domains.
N. Ayoub, I. Goldshmidt, and A. Cohen (1999)
Genetics 152, 495-508
   Abstract »    Full Text »
A Nonessential HP1-Like Protein Affects Starvation-Induced Assembly of Condensed Chromatin and Gene Expression in Macronuclei of Tetrahymena thermophila.
H. Huang, J. F. Smothers, E. A. Wiley, and C. D. Allis (1999)
Mol. Cell. Biol. 19, 3624-3634
   Abstract »    Full Text »    PDF »
A New Member of the Sin3 Family of Corepressors Is Essential for Cell Viability and Required for Retroelement Propagation in Fission Yeast.
V. D. Dang, M. J. Benedik, K. Ekwall, J. Choi, R. C. Allshire, and H. L. Levin (1999)
Mol. Cell. Biol. 19, 2351-2365
   Abstract »    Full Text »    PDF »
Reduced dosage of a single fission yeast MCM protein causes genetic instability and S phase delay.
D. Liang, J. Hodson, and S. Forsburg (1999)
J. Cell Sci. 112, 559-567
   Abstract »    PDF »
Fission Yeast Bub1 Is a Mitotic Centromere Protein Essential for the Spindle Checkpoint and the Preservation of Correct Ploidy through Mitosis.
P. Bernard, K. Hardwick, and J.-P. Javerzat (1998)
J. Cell Biol. 143, 1775-1787
   Abstract »    Full Text »    PDF »
An HP1-like protein is missing from transcriptionally silent micronuclei of Tetrahymena.
H. Huang, E. A. Wiley, C. R. Lending, and C. D. Allis (1998)
PNAS 95, 13624-13629
   Abstract »    Full Text »    PDF »
Histone Deacetylase Homologs Regulate Epigenetic Inheritance of Transcriptional Silencing and Chromosome Segregation in Fission Yeast.
S. I. S. Grewal, M. J. Bonaduce, and A. J. S. Klar (1998)
Genetics 150, 563-576
   Abstract »    Full Text »
The fission yeast microtubule cytoskeleton.
I. Hagan (1998)
J. Cell Sci. 111, 1603-1612
   Abstract »    PDF »
Purification and characterization of a CENP-B homologue protein that binds to the centromeric K-type repeat DNA of Schizosaccharomyces pombe.
J.-K. Lee, J. A. Huberman, and J. Hurwitz (1997)
PNAS 94, 8427-8432
   Abstract »    Full Text »    PDF »
A Centromere DNA-binding Protein from Fission Yeast Affects Chromosome Segregation and Has Homology to Human CENP-B.
D. Halverson, M. Baum, J. Stryker, J. Carbon, and L. Clarke (1997)
J. Cell Biol. 136, 487-500
   Abstract »    Full Text »    PDF »
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Q. Ye and H. J. Worman (1996)
J. Biol. Chem. 271, 14653-14656
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Mutations in the fission yeast silencing factors clr4+ and rik1+ disrupt the localisation of the chromo domain protein Swi6p and impair centromere function.
K Ekwall, E. Nimmo, J. Javerzat, B Borgstrom, R Egel, G Cranston, and R Allshire (1996)
J. Cell Sci. 109, 2637-2648
   Abstract »    PDF »
The Centromere: Hub of Chromosomal Activities.
A. F. Pluta, A. M. Mackay, A. M. Ainsztein, I. G. Goldberg, and W. C. Earnshaw (1995)
Science 270, 1591-1594
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