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Science 28 July 1995:
Vol. 269. no. 5223, pp. 538 - 540
DOI: 10.1126/science.7542802
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Articles

Science, Vol 269, Issue 5223, 538-540
Copyright © 1995 by American Association for the Advancement of Science

articles

Frequency and distribution of DNA uptake signal sequences in the Haemophilus influenzae Rd genome

HO Smith, JF Tomb, BA Dougherty, RD Fleischmann, and JC Venter

Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

The naturally transformable, Gram-negative bacterium Haemophilus influenzae Rd preferentially takes up DNA of its own species by recognizing a 9-base pair sequence, 5'-AAGTGCGGT, carried in multiple copies in its chromosome. With the availability of the complete genome sequence, 1465 copies of the 9-base pair uptake site have been identified. Alignment of these sites unexpectedly reveals an extended consensus region of 29 base pairs containing the core 9-base pair region and two downstream 6-base pair A/T-rich regions, each spaced about one helix turn apart. Seventeen percent of the sites are in inverted repeat pairs, many of which are located downstream to gene termini and are capable of forming stem-loop structures in messenger RNA that might function as signals for transcription termination.


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