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Science 21 July 1995: Vol. 269. no. 5222, pp. 400 - 403 DOI: 10.1126/science.7618105
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Articles
Science, Vol 269, Issue 5222, 400-403
Copyright © 1995 by American Association for the Advancement of Science
Simultaneous identification of bacterial virulence genes by negative selection
M Hensel,
JE Shea,
C Gleeson,
MD Jones,
E Dalton,
and
DW Holden
Department of Infectious Diseases and Bacteriology, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
An insertional mutagenesis system that uses transposons carrying unique DNA sequence tags was developed for the isolation of bacterial virulence genes. The tags from a mixed population of bacterial mutants representing the inoculum and bacteria recovered from infected hosts were detected by amplification, radiolabeling, and hybridization analysis. When applied to a murine model of typhoid fever caused by Salmonella typhimurium, mutants with attenuated virulence were revealed by use of tags that were present in the inoculum but not in bacteria recovered from infected mice. This approach resulted in the identification of new virulence genes, some of which are related to, but functionally distinct from, the inv/spa family of S. typhimurium.
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- Generation of Attenuated Mycobacterium bovis Strains by Signature-Tagged Mutagenesis for Discovery of Novel Vaccine Candidates.
- D. M. Collins, B. Skou, S. White, S. Bassett, L. Collins, R. For, K. Hurr, G. Hotter, and G. W. de Lisle (2005)
Infect. Immun.
73, 2379-2386
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- Designer Arrays for Defined Mutant Analysis To Detect Genes Essential for Survival of Mycobacterium tuberculosis in Mouse Lungs.
- G. Lamichhane, S. Tyagi, and W. R. Bishai (2005)
Infect. Immun.
73, 2533-2540
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- Generation of transposon insertion mutant libraries for Gram-positive bacteria by electroporation of phage Mu DNA transposition complexes.
- M. I. Pajunen, A. T. Pulliainen, J. Finne, and H. Savilahti (2005)
Microbiology
151, 1209-1218
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- Identification of Novel Cancer Drug Targets Using High Throughput RNAi Screens.
- R. Bernards (2005)
Am. Assoc. Cancer Res. Educ. Book
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- Identification of viral genes essential for replication of murine {gamma}-herpesvirus 68 using signature-tagged mutagenesis.
- M. J. Song, S. Hwang, W. H. Wong, T.-T. Wu, S. Lee, H.-I Liao, and R. Sun (2005)
PNAS
102, 3805-3810
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- Identification of Enterohemorrhagic Escherichia coli O26:H- Genes Required for Intestinal Colonization in Calves.
- P. M. van Diemen, F. Dziva, M. P. Stevens, and T. S. Wallis (2005)
Infect. Immun.
73, 1735-1743
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- Optimization of Virulence Functions Through Glucosylation of Shigella LPS.
- N. P. West, P. Sansonetti, J. Mounier, R. M. Exley, C. Parsot, S. Guadagnini, M.-C. Prevost, A. Prochnicka-Chalufour, M. Delepierre, M. Tanguy, et al. (2005)
Science
307, 1313-1317
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- Staphylococcus aureus Virulence Factors Identified by Using a High-Throughput Caenorhabditis elegans-Killing Model.
- J. Begun, C. D. Sifri, S. Goldman, S. B. Calderwood, and F. M. Ausubel (2005)
Infect. Immun.
73, 872-877
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- Large-Scale Screen Highlights the Importance of Capsule for Virulence in the Zoonotic Pathogen Streptococcus iniae.
- J. D. Miller and M. N. Neely (2005)
Infect. Immun.
73, 921-934
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- The Salmonella Pathogenicity Island (SPI)-2 and SPI-1 Type III Secretion Systems Allow Salmonella Serovar typhimurium to Trigger Colitis via MyD88-Dependent and MyD88-Independent Mechanisms.
- S. Hapfelmeier, B. Stecher, M. Barthel, M. Kremer, A. J. Muller, M. Heikenwalder, T. Stallmach, M. Hensel, K. Pfeffer, S. Akira, et al. (2005)
J. Immunol.
174, 1675-1685
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- Phenotypic Screening of Escherichia coli K-12 Tn5 Insertion Libraries, Using Whole-Genome Oligonucleotide Microarrays.
- K. M. Winterberg, J. Luecke, A. S. Bruegl, and W. S. Reznikoff (2005)
Appl. Envir. Microbiol.
71, 451-459
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- Dissecting Cancer Pathways and Vulnerabilities with RNAi.
- T.F. WESTBROOK, F. STEGMEIER, and S.J. ELLEDGE (2005)
Cold Spring Harb Symp Quant Biol
70, 435-444
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- Large-Scale Identification of Genes Required for Full Virulence of Staphylococcus aureus.
- B. M. Benton, J. P. Zhang, S. Bond, C. Pope, T. Christian, L. Lee, K. M. Winterberg, M. B. Schmid, and J. M. Buysse (2004)
J. Bacteriol.
186, 8478-8489
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- ATP-Binding Cassette Transporters Are Targets for the Development of Antibacterial Vaccines and Therapies.
- H. S. Garmory and R. W. Titball (2004)
Infect. Immun.
72, 6757-6763
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- Functional Characterization of Two Type III Secretion Systems of Vibrio parahaemolyticus.
- K.-S. Park, T. Ono, M. Rokuda, M.-H. Jang, K. Okada, T. Iida, and T. Honda (2004)
Infect. Immun.
72, 6659-6665
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- Identification of Escherichia coli O157 : H7 genes influencing colonization of the bovine gastrointestinal tract using signature-tagged mutagenesis.
- F. Dziva, P. M. van Diemen, M. P. Stevens, A. J. Smith, and T. S. Wallis (2004)
Microbiology
150, 3631-3645
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- Identification of Candidate Gammaherpesvirus 68 Genes Required for Virus Replication by Signature-Tagged Transposon Mutagenesis.
- N. J. Moorman, C. Y. Lin, and S. H. Speck (2004)
J. Virol.
78, 10282-10290
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- Molecular barcodes detect redundancy and contamination in hairpin-bisulfite PCR.
- B. E. Miner, R. J. Stoger, A. F. Burden, C. D. Laird, and R. S. Hansen (2004)
Nucleic Acids Res.
32, e135
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- Identification and Functional Characterization of a Xenorhabdus nematophila Oligopeptide Permease.
- S. S. Orchard and H. Goodrich-Blair (2004)
Appl. Envir. Microbiol.
70, 5621-5627
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- Identification of Mycobacterium tuberculosis Counterimmune (cim) Mutants in Immunodeficient Mice by Differential Screening.
- K. B. Hisert, M. A. Kirksey, J. E. Gomez, A. O. Sousa, J. S. Cox, W. R. Jacobs Jr., C. F. Nathan, and J. D. McKinney (2004)
Infect. Immun.
72, 5315-5321
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- Secretion and Function of Salmonella SPI-2 Effector SseF Require Its Chaperone, SscB.
- S. Dai and D. Zhou (2004)
J. Bacteriol.
186, 5078-5086
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- Delineation of Upstream Signaling Events in the Salmonella Pathogenicity Island 2 Transcriptional Activation Pathway.
- C. C. Kim and S. Falkow (2004)
J. Bacteriol.
186, 4694-4704
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- Identification and Characterization of a Novel Uropathogenic Escherichia coli-Associated Fimbrial Gene Cluster.
- E. L. Buckles, F. K. Bahrani-Mougeot, A. Molina, C. V. Lockatell, D. E. Johnson, C. B. Drachenberg, V. Burland, F. R. Blattner, and M. S. Donnenberg (2004)
Infect. Immun.
72, 3890-3901
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