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Science 23 June 1995: Vol. 268. no. 5218, pp. 1766 - 1769 DOI: 10.1126/science.7792603
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Articles
Science, Vol 268, Issue 5218, 1766-1769
Copyright © 1995 by American Association for the Advancement of Science
Transcriptional activation by tetracyclines in mammalian cells
M Gossen,
S Freundlieb,
G Bender,
G Muller,
W Hillen,
and
H Bujard
Zentrum fur Molekulare Biologie der Universitat Heidelberg, (ZMDH), Im Neuenheimer Feld 282, Germany.
A transcriptional transactivator was developed that fuses the VP16 activation domain with a mutant Tet repressor from Escherichia coli. This transactivator requires certain tetracycline (Tc) derivatives for specific DNA binding. Thus, addition of doxycycline to HeLa cells that constitutively synthesized the transactivator and that contained an appropriate, stably integrated reporter unit rapidly induced gene expression more than a thousandfold. The specificity of the Tet repressor-operator-effector interaction and the pharmacological characteristics of Tc's make this regulatory system well suited for the control of gene activities in vivo, such as in transgenic animals and possibly in gene therapy.
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- Persistence of Bovine Viral Diarrhea Virus Is Determined by a Cellular Cofactor of a Viral Autoprotease.
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- IRIP, a New Ischemia/Reperfusion-Inducible Protein That Participates in the Regulation of Transporter Activity.
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- Tetracycline-Inducible Gene Expression and Gene Deletion in Candida albicans.
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- SEI family of nuclear factors regulates p53-dependent transcriptional activation.
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Genes Cells
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- Interferon prevents formation of replication-competent hepatitis B virus RNA-containing nucleocapsids.
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- A novel mammalian expression system derived from components coordinating nicotine degradation in arthrobacter nicotinovorans pAO1.
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- Pituitary Adenylyl Cyclase-activating Polypeptide 38 Reduces Astroglial Proliferation by Inhibiting the GTPase RhoA.
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- Transgenic and Knockout Mice in Diabetes Research: Novel Insights into Pathophysiology, Limitations, and Perspectives.
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Physiology
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- Characterization of Tetracycline-Inducible Bitransgenic Krt12rtTA/+/tet-O-LacZ Mice.
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