Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 26 May 1995:
Vol. 268. no. 5214, pp. 1179 - 1183
DOI: 10.1126/science.7761835
Prev | Table of Contents | Next

Articles

Science, Vol 268, Issue 5214, 1179-1183
Copyright © 1995 by American Association for the Advancement of Science

articles

Inducible gene expression in trypanosomes mediated by a prokaryotic repressor

E Wirtz and C Clayton

Zentrum fur Molekulare Biologie, Universitat Heidelberg, Germany.

An inducible expression system was developed for the protozoan parasite Trypanosoma brucei. Transgenic trypanosomes expressing the tetracycline repressor of Escherichia coli exhibited inducer (tetracycline)-dependent expression of chromosomally integrated reporter genes under the control of a procyclic acidic repetitive protein (PARP) promoter bearing a tet operator. Reporter expression could be controlled over a range of four orders of magnitude in response to tetracycline concentration, a degree of regulation that exceeds those exhibited by other eukaryotic repression-based systems. The tet repressor-controlled PARP promoter should be a valuable tool for the study of trypanosome biochemistry, pathogenicity, and cell and molecular biology.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Tetracycline alters drug susceptibility in Candida albicans and other pathogenic fungi.
B. G. Oliver, P. M. Silver, C. Marie, S. J. Hoot, S. E. Leyde, and T. C. White (2008)
Microbiology 154, 960-970
   Abstract »    Full Text »    PDF »
Intraflagellar Transport and Functional Analysis of Genes Required for Flagellum Formation in Trypanosomes.
S. Absalon, T. Blisnick, L. Kohl, G. Toutirais, G. Dore, D. Julkowska, A. Tavenet, and P. Bastin (2008)
Mol. Biol. Cell 19, 929-944
   Abstract »    Full Text »    PDF »
Sphingolipid synthesis is necessary for kinetoplast segregation and cytokinesis in Trypanosoma brucei.
A. Fridberg, C. L. Olson, E. S. Nakayasu, K. M. Tyler, I. C. Almeida, and D. M. Engman (2008)
J. Cell Sci. 121, 522-535
   Abstract »    Full Text »    PDF »
Adenosine Kinase of Trypanosoma brucei and Its Role in Susceptibility to Adenosine Antimetabolites.
A. Luscher, P. Onal, A.-M. Schweingruber, and P. Maser (2007)
Antimicrob. Agents Chemother. 51, 3895-3901
   Abstract »    Full Text »    PDF »
Depletion of newly synthesized Argonaute1 impairs the RNAi response in Trypanosoma brucei.
H. Shi, C. Tschudi, and E. Ullu (2007)
RNA 13, 1132-1139
   Abstract »    Full Text »    PDF »
An Essential Cell Cycle-regulated Nucleolar Protein Relocates to the Mitotic Spindle Where It Is Involved in Mitotic Progression in Trypanosoma brucei.
N. Boucher, D. Dacheux, C. Giroud, and T. Baltz (2007)
J. Biol. Chem. 282, 13780-13790
   Abstract »    Full Text »    PDF »
Mitochondrial Fatty Acid Synthesis in Trypanosoma brucei.
J. L. Stephens, S. H. Lee, K. S. Paul, and P. T. Englund (2007)
J. Biol. Chem. 282, 4427-4436
   Abstract »    Full Text »    PDF »
Knock-downs of Iron-Sulfur Cluster Assembly Proteins IscS and IscU Down-regulate the Active Mitochondrion of Procyclic Trypanosoma brucei.
O. Smid, E. Horakova, V. Vilimova, I. Hrdy, R. Cammack, A. Horvath, J. Lukes, and J. Tachezy (2006)
J. Biol. Chem. 281, 28679-28686
   Abstract »    Full Text »    PDF »
Molecular Characterization of Trypanosoma brucei P-type H+-ATPases.
S. Luo, J. Fang, and R. Docampo (2006)
J. Biol. Chem. 281, 21963-21973
   Abstract »    Full Text »    PDF »
Overexpression of a Cytochrome b5 Reductase-like Protein Causes Kinetoplast DNA Loss in Trypanosoma brucei.
S. A. Motyka, M. E. Drew, G. Yildirir, and P. T. Englund (2006)
J. Biol. Chem. 281, 18499-18506
   Abstract »    Full Text »    PDF »
Experimental and in Silico Analyses of Glycolytic Flux Control in Bloodstream Form Trypanosoma brucei.
M.-A. Albert, J. R. Haanstra, V. Hannaert, J. Van Roy, F. R. Opperdoes, B. M. Bakker, and P. A. M. Michels (2005)
J. Biol. Chem. 280, 28306-28315
   Abstract »    Full Text »    PDF »
Mitochondrial Initiation Factor 2 of Trypanosoma brucei Binds Imported Formylated Elongator-type tRNAMet.
F. Charriere, T. H. P. Tan, and A. Schneider (2005)
J. Biol. Chem. 280, 15659-15665
   Abstract »    Full Text »    PDF »
Tetracycline analogue-regulated transgene expression in Plasmodium falciparum blood stages using Toxoplasma gondii transactivators.
M. Meissner, E. Krejany, P. R. Gilson, T. F. de Koning-Ward, D. Soldati, and B. S. Crabb (2005)
PNAS 102, 2980-2985
   Abstract »    Full Text »    PDF »
Regulated gene expression by glucocorticoids in cultured Virginia pine (Pinus virginiana Mill.) cells.
W. Tang and R. J. Newton (2004)
J. Exp. Bot. 55, 1499-1508
   Abstract »    Full Text »    PDF »
Protein Targeting of an Unusual, Evolutionarily Conserved Adenylate Kinase to a Eukaryotic Flagellum.
T. J. Pullen, M. L. Ginger, S. J. Gaskell, and K. Gull (2004)
Mol. Biol. Cell 15, 3257-3265
   Abstract »    Full Text »    PDF »
The Small Chromosomes of Trypanosoma brucei Involved in Antigenic Variation Are Constructed Around Repetitive Palindromes.
B. Wickstead, K. Ersfeld, and K. Gull (2004)
Genome Res. 14, 1014-1024
   Abstract »    Full Text »    PDF »
Trypanosoma brucei Plasma Membrane-Type Ca2+-ATPase 1 (TbPMC1) and 2 (TbPMC2) Genes Encode Functional Ca2+-ATPases Localized to the Acidocalcisomes and Plasma Membrane, and Essential for Ca2+ Homeostasis and Growth.
S. Luo, P. Rohloff, J. Cox, S. A. Uyemura, and R. Docampo (2004)
J. Biol. Chem. 279, 14427-14439
   Abstract »    Full Text »    PDF »
The frequency of gene targeting in Trypanosoma brucei is independent of target site copy number.
B. Wickstead, K. Ersfeld, and K. Gull (2003)
Nucleic Acids Res. 31, 3993-4000
   Abstract »    Full Text »    PDF »
Ex Vivo and In Vitro Identification of a Consensus Promoter for VSG Genes Expressed by Metacyclic-Stage Trypanosomes in the Tsetse Fly.
M. L. Ginger, P. A. Blundell, A. M. Lewis, A. Browitt, A. Gunzl, and J. D. Barry (2002)
Eukaryot. Cell 1, 1000-1009
   Abstract »    Full Text »    PDF »
Expression of the human DNA glycosylase hSMUG1 in Trypanosoma brucei causes DNA damage and interferes with J biosynthesis.
S. Ulbert, M. Cross, R. J. Boorstein, G. W. Teebor, and P. Borst (2002)
Nucleic Acids Res. 30, 3919-3926
   Abstract »    Full Text »    PDF »
Mitochondrial Substrate Level Phosphorylation Is Essential for Growth of Procyclic Trypanosoma brucei.
N. Bochud-Allemann and A. Schneider (2002)
J. Biol. Chem. 277, 32849-32854
   Abstract »    Full Text »    PDF »
Trypanin is a cytoskeletal linker protein and is required for cell motility in African trypanosomes.
N. R. Hutchings, J. E. Donelson, and K. L. Hill (2002)
J. Cell Biol. 156, 867-877
   Abstract »    Full Text »    PDF »
A novel GFP approach for the analysis of genetic exchange in trypanosomes allowing the in situ detection of mating events.
L. E. H. Bingle, J. L. Eastlake, M. Bailey, and W. C. Gibson (2001)
Microbiology 147, 3231-3240
   Abstract »    Full Text »    PDF »
Degradation of the unstable EP1 mRNA in Trypanosoma brucei involves initial destruction of the 3'-untranslated region.
H. Irmer and C. Clayton (2001)
Nucleic Acids Res. 29, 4707-4715
   Abstract »    Full Text »    PDF »
Modulation of myosin A expression by a newly established tetracycline repressor-based inducible system in Toxoplasma gondii.
M. Meissner, S. Brecht, H. Bujard, and D. Soldati (2001)
Nucleic Acids Res. 29, e115
   Abstract »    Full Text »    PDF »
Targeting of cytoskeletal proteins to the flagellum of Trypanosoma brucei.
K Ersfeld and K Gull (2001)
J. Cell Sci. 114, 141-148
   Abstract »    PDF »
Isolation of Trypanosoma brucei CYC2 and CYC3 Cyclin Genes by Rescue of a Yeast G1 Cyclin Mutant. FUNCTIONAL CHARACTERIZATION OF CYC2.
J. J. Van Hellemond, P. Neuville, R. T. Schwarz, K. R. Matthews, and J. C. Mottram (2000)
J. Biol. Chem. 275, 8315-8323
   Abstract »    Full Text »    PDF »
Flagellum ontogeny in trypanosomes studied via an inherited and regulated RNA interference system.
P Bastin, K Ellis, L Kohl, and K Gull (2000)
J. Cell Sci. 113, 3321-3328
   Abstract »    PDF »
Flagellar Morphogenesis: Protein Targeting and Assembly in the Paraflagellar Rod of Trypanosomes.
P. Bastin, T. H. MacRae, S. B. Francis, K. R. Matthews, and K. Gull (1999)
Mol. Cell. Biol. 19, 8191-8200
   Abstract »    Full Text »    PDF »
Double-stranded RNA induces mRNA degradation in Trypanosoma brucei.
H. Ngo, C. Tschudi, K. Gull, and E. Ullu (1998)
PNAS 95, 14687-14692
   Abstract »    Full Text »    PDF »
Activity of a Trypanosome Metacyclic Variant Surface Glycoprotein Gene Promoter Is Dependent upon Life Cycle Stage and Chromosomal Context.
S. V. Graham, B. Wymer, and J. D. Barry (1998)
Mol. Cell. Biol. 18, 1137-1146
   Abstract »    Full Text »
Inhibition of Trypanosoma brucei Gene Expression by RNA Interference Using an Integratable Vector with Opposing T7 Promoters.
Z. Wang, J. C. Morris, M. E. Drew, and P. T. Englund (2000)
J. Biol. Chem. 275, 40174-40179
   Abstract »    Full Text »    PDF »
Trypanin is a cytoskeletal linker protein and is required for cell motility in African trypanosomes.
N. R. Hutchings, J. E. Donelson, and K. L. Hill (2002)
J. Cell Biol. 156, 867-877
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)