Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 14 April 1995:
Vol. 268. no. 5208, pp. 304 - 307
DOI: 10.1126/science.7716526
Prev | Table of Contents | Next

Articles

Science, Vol 268, Issue 5208, 304-307
Copyright © 1995 by American Association for the Advancement of Science

articles

Silver as a probe of pore-forming residues in a potassium channel

Q Lu and C Miller

Howard Hughes Medical Institute, Brandeis University, Waltham, MA 02254, USA.

In voltage-dependent potassium channels, the molecular determinants of ion selectivity are found in the P (pore) region, a stretch of 21 contiguous residues. Cysteine was introduced at each P region position in a Shaker potassium channel. Residues projecting side chains into the pore were identified by means of channel inhibition by a sulfhydryl-reactive potassium ion analog, silver ion. The pattern of silver ion reactivity contradicts a beta barrel architecture of potassium channel pores.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Aqueous Accessibility to the Transmembrane Regions of Subunit c of the Escherichia coli F1F0 ATP Synthase.
P. R. Steed and R. H. Fillingame (2009)
J. Biol. Chem. 284, 23243-23250
   Abstract »    Full Text »    PDF »
Hydrophobic Interactions as Key Determinants to the KCa3.1 Channel Closed Configuration: AN ANALYSIS OF KCa3.1 MUTANTS CONSTITUTIVELY ACTIVE IN ZERO Ca2+.
L. Garneau, H. Klein, U. Banderali, A. Longpre-Lauzon, L. Parent, and R. Sauve (2009)
J. Biol. Chem. 284, 389-403
   Abstract »    Full Text »    PDF »
The Cytoplasmic Loops of Subunit a of Escherichia coli ATP Synthase May Participate in the Proton Translocating Mechanism.
K. J. Moore, C. M. Angevine, O. D. Vincent, B. E. Schwem, and R. H. Fillingame (2008)
J. Biol. Chem. 283, 13044-13052
   Abstract »    Full Text »    PDF »
Subunit a Facilitates Aqueous Access to a Membrane-embedded Region of Subunit c in Escherichia coli F1F0 ATP Synthase.
P. R. Steed and R. H. Fillingame (2008)
J. Biol. Chem. 283, 12365-12372
   Abstract »    Full Text »    PDF »
Gating at the selectivity filter in cyclic nucleotide-gated channels.
J. E. Contreras, D. Srikumar, and M. Holmgren (2008)
PNAS 105, 3310-3314
   Abstract »    Full Text »    PDF »
Aqueous Access Pathways in ATP Synthase Subunit a: REACTIVITY OF CYSTEINE SUBSTITUTED INTO TRANSMEMBRANE HELICES 1, 3, AND 5.
C. M. Angevine, K. A. G. Herold, O. D. Vincent, and R. H. Fillingame (2007)
J. Biol. Chem. 282, 9001-9007
   Abstract »    Full Text »    PDF »
Cross-linking between Helices within Subunit a of Escherichia coli ATP Synthase Defines the Transmembrane Packing of a Four-helix Bundle.
B. E. Schwem and R. H. Fillingame (2006)
J. Biol. Chem. 281, 37861-37867
   Abstract »    Full Text »    PDF »
Y3+ Block Demonstrates an Intracellular Activation Gate for the {alpha}1G T-type Ca2+ Channel.
C. A. Obejero-Paz, I. P. Gray, and S. W. Jones (2004)
J. Gen. Physiol. 124, 631-640
   Abstract »    Full Text »    PDF »
Outer Pore Architecture of a Ca2+-selective TRP Channel.
T. Voets, A. Janssens, G. Droogmans, and B. Nilius (2004)
J. Biol. Chem. 279, 15223-15230
   Abstract »    Full Text »    PDF »
Analysis of Transmembrane Segment 7 of the Dipeptide Transporter hPepT1 by Cysteine-scanning Mutagenesis.
A. A. Kulkarni, I. S. Haworth, T. Uchiyama, and V. H. L. Lee (2003)
J. Biol. Chem. 278, 51833-51840
   Abstract »    Full Text »    PDF »
Aqueous access pathways in subunit a of rotary ATP synthase extend to both sides of the membrane.
C. M. Angevine, K. A. G. Herold, and R. H. Fillingame (2003)
PNAS 100, 13179-13183
   Abstract »    Full Text »    PDF »
Probing the Pore of ClC-0 by Substituted Cysteine Accessibility Method Using Methane Thiosulfonate Reagents.
C.-W. Lin and T.-Y. Chen (2003)
J. Gen. Physiol. 122, 147-159
   Abstract »    Full Text »    PDF »
Aqueous Access Channels in Subunit a of Rotary ATP Synthase.
C. M. Angevine and R. H. Fillingame (2003)
J. Biol. Chem. 278, 6066-6074
   Abstract »    Full Text »    PDF »
An External Determinant in the S5-P Linker of the Pacemaker (HCN) Channel Identified by Sulfhydryl Modification.
T. Xue and R. A. Li (2002)
J. Biol. Chem. 277, 46233-46242
   Abstract »    Full Text »    PDF »
Kinetics of Tethering Quaternary Ammonium Compounds to K+ Channels.
R. O. Blaustein (2002)
J. Gen. Physiol. 120, 203-216
   Abstract »    Full Text »    PDF »
Cyclic Nucleotide-Gated Ion Channels.
U. B. Kaupp and R. Seifert (2002)
Physiol Rev 82, 769-824
   Abstract »    Full Text »    PDF »
On the Contribution of the First Transmembrane Domain to Whole-Cell Current through an ATP-Gated Ionotropic P2X Receptor.
W. R. Haines, M. M. Voigt, K. Migita, G. E. Torres, and T. M. Egan (2001)
J. Neurosci. 21, 5885-5892
   Abstract »    Full Text »    PDF »
Molecular Architecture of the Voltage-Dependent Na Channel: Functional Evidence for {alpha} Helices in the Pore.
T. Yamagishi, R. A. Li, K. Hsu, E. Marban, and G. F. Tomaselli (2001)
J. Gen. Physiol. 118, 171-182
   Abstract »    Full Text »    PDF »
Mechanism of Ba2+ block of a mouse inwardly rectifying K+ channel: differential contribution by two discrete residues.
N. Alagem, M. Dvir, and E. Reuveny (2001)
J. Physiol. 534, 381-393
   Abstract »    Full Text »    PDF »
Structural Similarities between Glutamate Receptor Channels and K+ Channels Examined by Scanning Mutagenesis.
V. A. Panchenko, C. R. Glasser, and M. L. Mayer (2001)
J. Gen. Physiol. 117, 345-360
   Abstract »    Full Text »    PDF »
Scam Feels the Pinch.
A. Karlin (2001)
J. Gen. Physiol. 117, 235-238
   Full Text »    PDF »
The K+ channel signature sequence of murine Kir2.1: mutations that affect microscopic gating but not ionic selectivity.
I So, I Ashmole, N W Davies, M J Sutcliffe, and P R Stanfield (2001)
J. Physiol. 531, 37-50
   Abstract »    Full Text »    PDF »
Residues beyond the selectivity filter of the K+ channel Kir2.1 regulate permeation and block by external Rb+ and Cs+.
G A Thompson, M L Leyland, I Ashmole, M J Sutcliffe, and P R Stanfield (2000)
J. Physiol. 526, 231-240
   Abstract »    Full Text »    PDF »
Novel Gain-of-Function Mechanism in K+ Channel-Related Long-QT Syndrome: : Altered Gating and Selectivity in the HERG1 N629D Mutant.
J. P. Lees-Miller, Y. Duan, G. Q. Teng, K. Thorstad, and H. J. Duff (2000)
Circ. Res. 86, 507-513
   Abstract »    Full Text »    PDF »
Structure and Dynamics of the Pore of Inwardly Rectifying KATP Channels.
G. Loussouarn, E. N. Makhina, T. Rose, and C. G. Nichols (2000)
J. Biol. Chem. 275, 1137-1144
   Abstract »    Full Text »    PDF »
Cyclic Nucleotide-Gated Channels: Pore Topology Studied through the Accessibility of Reporter Cysteines.
A. Becchetti, K. Gamel, and V. Torre (1999)
J. Gen. Physiol. 114, 377-392
   Abstract »    Full Text »    PDF »
Molecular Dynamics of the Sodium Channel Pore Vary with Gating: Interactions between P-Segment Motions and Inactivation.
J.-P. Benitah, Z. Chen, J. R. Balser, G. F. Tomaselli, and E. Marban (1999)
J. Neurosci. 19, 1577-1585
   Abstract »    Full Text »    PDF »
The Nicotinic alpha 4 Receptor Subunit Contributes to the Lining of the Ion Channel Pore When Expressed with the 5-HT3 Receptor Subunit.
S. Kriegler, S. Sudweeks, and J. L. Yakel (1999)
J. Biol. Chem. 274, 3934-3936
   Abstract »    Full Text »    PDF »
Functional Consequences of a Decreased Potassium Affinity in a Potassium Channel Pore: Ion Interactions and C-Type Inactivation.
E. M. Ogielska and R. W. Aldrich (1999)
J. Gen. Physiol. 113, 347-358
   Abstract »    Full Text »    PDF »
Heteromultimeric Potassium Channels Formed by Members of the Kv2 Subfamily.
J. T. Blaine and A. B. Ribera (1998)
J. Neurosci. 18, 9585-9593
   Abstract »    Full Text »    PDF »
Differential Effects of S6 Mutations on Binding of Quinidine and 4-Aminopyridine to Rat Isoform of Kv1.4: Common Site but Different Factors in Determining Blockers' Binding Affinity.
H. Zhang, B. Zhu, J.-A. Yao, and G.-N. Tseng (1998)
J. Pharmacol. Exp. Ther. 287, 332-343
   Abstract »    Full Text »
Cys-scanning mutagenesis: a novel approach to structure–function relationships in polytopic membrane proteins.
S. Frillingos, M. Sahin-tóth, J. Wu, and H. R. Kaback (1998)
FASEB J 12, 1281-1299
   Abstract »    Full Text »
The dependence of Ag+ block of a potassium channel, murine Kir2.1, on a cysteine residue in the selectivity filter.
C Dart, M L Leyland, R Barrett-Jolley, P A Shelton, P J Spencer, E C Conley, M J Sutcliffe, and P R Stanfield (1998)
J. Physiol. 511, 15-24
   Abstract »    Full Text »    PDF »
The selectivity filter of a potassium channel, murine Kir2.1, investigated using scanning cysteine mutagenesis.
C Dart, M L Leyland, P J Spencer, P R Stanfield, and M J Sutcliffe (1998)
J. Physiol. 511, 25-32
   Abstract »    Full Text »    PDF »
State-dependent Accessibility and Electrostatic Potential in the Channel of the Acetylcholine Receptor: Inferences from Rates of Reaction of Thiosulfonates with Substituted Cysteines in the M2 Segment of the {alpha} Subunit.
J. M. Pascual and A. Karlin (1998)
J. Gen. Physiol. 111, 717-739
   Abstract »    Full Text »    PDF »
Fourier transform infrared spectroscopy reveals a rigid alpha -helical assembly for the tetrameric Streptomyces lividans K+ channel.
J. le Coutre, H. R. Kaback, C. K. N. Patel, L. Heginbotham, and C. Miller (1998)
PNAS 95, 6114-6117
   Abstract »    Full Text »    PDF »
The Selectivity Filter of the N-Methyl-D-Aspartate Receptor: A Tryptophan Residue Controls Block and Permeation of Mg2+.
K. Williams, A. J. Pahk, K. Kashiwagi, T. Masuko, N. D. Nguyen, and K. Igarashi (1998)
Mol. Pharmacol. 53, 933-941
   Abstract »    Full Text »
A Domain Contributing to the Ion Channel of ATP-Gated P2X2 Receptors Identified by the Substituted Cysteine Accessibility Method.
T. M. Egan, W. R. Haines, and M. M. Voigt (1998)
J. Neurosci. 18, 2350-2359
   Abstract »    Full Text »    PDF »
Specific Antibodies to the External Vestibule of Voltage-gated Potassium Channels Block Current.
B.-Y. Zhou, W. Ma, and X.-Y. Huang (1998)
J. Gen. Physiol. 111, 555-563
   Abstract »    Full Text »    PDF »
Probing the Structure of the Diphtheria Toxin Channel: Reactivity in Planar Lipid Bilayer Membranes of Cysteine-substituted Mutant Channels with Methanethiosulfonate Derivatives.
P. D. Huynh, C. Cui, H. Zhan, K. J. Oh, R. J. Collier, and A. Finkelstein (1997)
J. Gen. Physiol. 110, 229-242
   Abstract »    Full Text »    PDF »
Selectivity Changes during Activation of Mutant Shaker Potassium Channels.
J. Zheng and F. J. Sigworth (1997)
J. Gen. Physiol. 110, 101-117
   Abstract »    Full Text »    PDF »
How Does the W434F Mutation Block Current in Shaker Potassium Channels?.
Y. Yang, Y. Yan, and F. J. Sigworth (1997)
J. Gen. Physiol. 109, 779-789
   Abstract »    Full Text »    PDF »
Scanning mutagenesis of the putative transmembrane segments of Kir2.1, an inward rectifier potassium channel.
A. Collins, H.-h. Chuang, Y. N. Jan, and L. Y. Jan (1997)
PNAS 94, 5456-5460
   Abstract »    Full Text »    PDF »
Separation of Gating Properties from Permeation and Block in mslo Large Conductance Ca-activated K+ Channels.
D.H. Cox, J. Cui, and R.W. Aldrich (1997)
J. Gen. Physiol. 109, 633-646
   Abstract »    Full Text »    PDF »
Altered Ionic Selectivity of the Sodium Channel Revealed by Cysteine Mutations within the Pore.
R. G. Tsushima, R. A. Li, and P. H. Backx (1997)
J. Gen. Physiol. 109, 463-475
   Abstract »    Full Text »    PDF »
Stabilization of ion selectivity filter by pore loop ion pairs in an inwardly rectifying potassium channel.
J. Yang, M. Yu, Y. N. Jan, and L. Y. Jan (1997)
PNAS 94, 1568-1572
   Abstract »    Full Text »    PDF »
Determination of Key Structural Requirements of a K+ Channel Pore.
R. L. Nakamura, J. A. Anderson, and R. F. Gaber (1997)
J. Biol. Chem. 272, 1011-1018
   Abstract »    Full Text »    PDF »
Identification of Residues in the Translocation Pathway of EmrE, a Multidrug Antiporter from Escherichia coli.
M. Lebendiker and S. Schuldiner (1996)
J. Biol. Chem. 271, 21193-21199
   Abstract »    Full Text »    PDF »
Enzymatic and Biochemical Probes of Residues External to the Translocation Pathway of UhpT, the Sugar Phosphate Carrier of Escherichia coli.
M. Matos, M.-C. Fann, R.-T. Yan, and P. C. Maloney (1996)
J. Biol. Chem. 271, 18571-18575
   Abstract »    Full Text »    PDF »
Side Chain Orientation in the Selectivity Filter of a Voltage-gated Ca2+ Channel.
X.-S. Wu, H. D. Edwards, and W. A. Sather (2000)
J. Biol. Chem. 275, 31778-31785
   Abstract »    Full Text »    PDF »
Epithelial Sodium Channel Pore Region. STRUCTURE AND ROLE IN GATING.
S. Sheng, J. Li, K. A. McNulty, T. Kieber-Emmons, and T. R. Kleyman (2001)
J. Biol. Chem. 276, 1326-1334
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)