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Science 27 January 1995:
Vol. 267. no. 5197, pp. 515 - 518
DOI: 10.1126/science.7824949
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Articles

Science, Vol 267, Issue 5197, 515-518
Copyright © 1995 by American Association for the Advancement of Science

articles

Zeta phosphorylation without ZAP-70 activation induced by TCR antagonists or partial agonists

J Madrenas, RL Wange, JL Wang, N Isakov, LE Samelson, and RN Germain

Lymphocyte Biology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.

Small changes in the peptide-major histocompatibility complex (MHC) molecule ligands recognized by antigen-specific T cell receptors (TCRs) can convert fully activating complexes into partially activating or even inhibitory ones. This study examined early TCR-dependent signals induced by such partial agonists or antagonists. In contrast to typical agonist ligands, both an antagonist and several partial agonists stimulated a distinct pattern of zeta chain phosphorylation and failed to activate associated ZAP-70 kinase. These results identify a specific step in the early tyrosine phosphorylation cascade that is altered after TCR engagement with modified peptide-MHC molecule complexes. This finding may explain the different biological responses to TCR occupancy by these variant ligands.


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   Abstract »    Full Text »    PDF »
Interleukin 2-mediated Uncoupling of T Cell Receptor {alpha}/{beta} from CD3 Signaling.
L. Haughn, B. Leung, L. Boise, A. Veillette, C. Thompson, and M. Julius (1998)
J. Exp. Med. 188, 1575-1586
   Abstract »    Full Text »    PDF »
The Importance of Pairwise Interactions Between Peptide Residues in the Delineation of TCR Specificity.
G. R. Leggatt, A. Hosmalin, C. D. Pendleton, A. Kumar, S. Hoffman, and J. A. Berzofsky (1998)
J. Immunol. 161, 4728-4735
   Abstract »    Full Text »    PDF »
Structural Aspects of the Interaction Between Heterogeneic Human Papillomavirus Type 1 E4-Specific T Cell Receptors and the Same Peptide/HLA-DQ8 Complex.
J. C. Steele, S. P. Young, J. C. Goodall, and P. H. Gallimore (1998)
J. Immunol. 161, 4745-4752
   Abstract »    Full Text »    PDF »
Characterization of a Peptide Analog of a Determinant of Type II Collagen That Suppresses Collagen-Induced Arthritis.
L. K. Myers, B. Tang, E. F. Rosloniec, J. M. Stuart, T. M. Chiang, and A. H. Kang (1998)
J. Immunol. 161, 3589-3595
   Abstract »    Full Text »    PDF »
Sustained TCR Signaling Is Required for Mitogen-Activated Protein Kinase Activation and Degranulation by Cytotoxic T Lymphocytes.
N. N. Berg, L. G. Puente, W. Dawicki, and H. L. Ostergaard (1998)
J. Immunol. 161, 2919-2924
   Abstract »    Full Text »    PDF »
ZAP-70 Tyrosine Kinase Is Required for LFA-1-dependent T Cell Migration.
R. D.M. Soede, Y. M. Wijnands, I. Van Kouteren-Cobzaru, and E. Roos (1998)
J. Cell Biol. 142, 1371-1379
   Abstract »    Full Text »    PDF »
Tyrosine 474 of ZAP-70 Is Required for Association with the Shc Adaptor and for T-cell Antigen Receptor-dependent Gene Activation.
S. Pacini, C. Ulivieri, M. M. Di Somma, A. Isacchi, L. Lanfrancone, P. G. Pelicci, J. L. Telford, and C. T. Baldari (1998)
J. Biol. Chem. 273, 20487-20493
   Abstract »    Full Text »    PDF »
CD4 Regulation of TCR Signaling and T Cell Differentiation Following Stimulation with Peptides of Different Affinities for the TCR.
D. Leitenberg, Y. Boutin, S. Constant, and K. Bottomly (1998)
J. Immunol. 161, 1194-1203
   Abstract »    Full Text »    PDF »
An Unusual Mechanism for Ligand Antagonism.
C. Torigoe, J. K. Inman, and H. Metzger (1998)
Science 281, 568-572
   Abstract »    Full Text »
TCR Agonist and Antagonist Exert In Vivo Cross-Regulation When Presented on Igs.
K. L. Legge, B. Min, A. E. Cestra, C. D. Pack, and H. Zaghouani (1998)
J. Immunol. 161, 106-111
   Abstract »    Full Text »    PDF »
In Vivo Expression of a TCR Antagonist: T Cells Escape Central Tolerance But Are Antagonized in the Periphery.
C. B. Williams, K. Vidal, D. Donermeyer, D. A. Peterson, J. M. White, and P. M. Allen (1998)
J. Immunol. 161, 128-137
   Abstract »    Full Text »    PDF »
A Partially Modified Retro-Inverso Pseudopeptide Modulates the Cytokine Profile of CTL Specific for an Influenza Virus Epitope.
M. Ostankovitch, G. Guichard, F. Connan, S. Muller, A. Chaboissier, J. Hoebeke, J. Choppin, J.-P. Briand, and J.-G. Guillet (1998)
J. Immunol. 161, 200-208
   Abstract »    Full Text »    PDF »
T Cell Activation Induced by Novel Gain-of-function Mutants of Syk and ZAP-70.
L. Zeitlmann, T. Knorr, M. Knoll, C. Romeo, P. Sirim, and W. Kolanus (1998)
J. Biol. Chem. 273, 15445-15452
   Abstract »    Full Text »    PDF »
Increased Threshold for TCR-Mediated Signaling Controls Self Reactivity of Intraepithelial Lymphocytes.
S. R. Guehler, R. J. Finch, J. A. Bluestone, and T. A. Barrett (1998)
J. Immunol. 160, 5341-5346
   Abstract »    Full Text »    PDF »
Dissociation of Intracellular Signaling Pathways in Response to Partial Agonist Ligands of the T Cell Receptor.
L. A. Chau, J. A. Bluestone, and J. Madrenas (1998)
J. Exp. Med. 187, 1699-1709
   Abstract »    Full Text »    PDF »


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