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Science 13 January 1995:
Vol. 267. no. 5195, pp. 240 - 243
DOI: 10.1126/science.267.5195.240
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Articles

Metabolic Engineering of a Pentose Metabolism Pathway in Ethanologenic Zymomonas mobilis

Min Zhang 1, Christina Eddy 1, Kristine Deanda 1, Mark Finkelstein 1, and Stephen Picataggio 1

1 National Renewable Energy Laboratory, Applied Biological Sciences Branch, Golden, CO 80401, USA

The ethanol-producing bacterium Zymomonas mobilis was metabolically engineered to broaden its range of fermentable substrates to include the pentose sugar xylose. Two operons encoding xylose assimilation and pentose phosphate pathway enzymes were constructed and transformed into Z. mobilis in order to generate a strain that grew on xylose and efficiently fermented it to ethanol. Thus, anaerobic fermentation of a pentose sugar to ethanol was achieved through a combination of the pentose phosphate and Entner-Doudoroff pathways. Furthermore, this strain efficiently fermented both glucose and xylose, which is essential for economical conversion of lignocellulosic biomass to ethanol.

Submitted on August 2, 1994
Accepted on November 7, 1994


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Complete Genome Sequence of the Ethanol Producer Zymomonas mobilis NCIMB 11163.
V. N. Kouvelis, E. Saunders, T. S. Brettin, D. Bruce, C. Detter, C. Han, M. A. Typas, and K. M. Pappas (2009)
J. Bacteriol. 191, 7140-7141
   Abstract »    Full Text »    PDF »
Metabolic engineering of a thermophilic bacterium to produce ethanol at high yield.
A. J. Shaw, K. K. Podkaminer, S. G. Desai, J. S. Bardsley, S. R. Rogers, P. G. Thorne, D. A. Hogsett, and L. R. Lynd (2008)
PNAS 105, 13769-13774
   Abstract »    Full Text »    PDF »
Engineering Pseudomonas putida S12 for Efficient Utilization of D-Xylose and L-Arabinose.
J.-P. Meijnen, J. H. de Winde, and H. J. Ruijssenaars (2008)
Appl. Envir. Microbiol. 74, 5031-5037
   Abstract »    Full Text »    PDF »
Minimal Escherichia coli Cell for the Most Efficient Production of Ethanol from Hexoses and Pentoses.
C. T. Trinh, P. Unrean, and F. Srienc (2008)
Appl. Envir. Microbiol. 74, 3634-3643
   Abstract »    Full Text »    PDF »
Genetic Engineering of Zymobacter palmae for Production of Ethanol from Xylose.
H. Yanase, D. Sato, K. Yamamoto, S. Matsuda, S. Yamamoto, and K. Okamoto (2007)
Appl. Envir. Microbiol. 73, 2592-2599
   Abstract »    Full Text »    PDF »
Challenges in Engineering Microbes for Biofuels Production.
G. Stephanopoulos (2007)
Science 315, 801-804
   Abstract »    Full Text »    PDF »
High-Level Acetaldehyde Production in Lactococcus lactis by Metabolic Engineering.
R. S. Bongers, M. H. N. Hoefnagel, and M. Kleerebezem (2005)
Appl. Envir. Microbiol. 71, 1109-1113
   Abstract »    Full Text »    PDF »
A Modified Saccharomyces cerevisiae Strain That Consumes L-Arabinose and Produces Ethanol.
J. Becker and E. Boles (2003)
Appl. Envir. Microbiol. 69, 4144-4150
   Abstract »    Full Text »    PDF »
Microbial Cellulose Utilization: Fundamentals and Biotechnology.
L. R. Lynd, P. J. Weimer, W. H. van Zyl, and I. S. Pretorius (2002)
Microbiol. Mol. Biol. Rev. 66, 506-577
   Abstract »    Full Text »    PDF »
Kinetic and Nuclear Magnetic Resonance Studies of Xylose Metabolism by Recombinant Zymomonas mobilis ZM4(pZB5).
I. S. Kim, K. D. Barrow, and P. L. Rogers (2000)
Appl. Envir. Microbiol. 66, 186-193
   Abstract »    Full Text »
Ethanol Synthesis by Genetic Engineering in Cyanobacteria.
M.-D. Deng and J. R. Coleman (1999)
Appl. Envir. Microbiol. 65, 523-528
   Abstract »    Full Text »


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