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Science 23 December 1994:
Vol. 266. no. 5193, pp. 1989 - 1992
DOI: 10.1126/science.7801125
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Articles

Science, Vol 266, Issue 5193, 1989-1992
Copyright © 1994 by American Association for the Advancement of Science

articles

A receptor component of the chloroplast protein translocation machinery

S Hirsch, E Muckel, F Heemeyer, G von Heijne, and J Soll

Botanisches Institut, Universitat Kiel, Germany.

The chloroplast outer envelope protein OEP86 functions as a receptor in precursor protein translocation into chloroplasts. Sequence analysis suggests that the precursor of OEP86 is directed to the chloroplast outer envelope by a cleavable, negatively charged, and unusually long amino-terminal peptide. This presequence is unlike other potential targeting signals and suggests the existence of another membrane insertion pathway. Insertion of precursor OEP86 required the hydrolysis of adenosine triphosphate and the existence of surface exposed chloroplast membrane components, and it was not competed by another precursor protein destined for the internal plastid compartments.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Dimerization Is Important for the GTPase Activity of Chloroplast Translocon Components atToc33 and psToc159.
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Characterization of the Preprotein Translocon at the Outer Envelope Membrane of Chloroplasts by Blue Native PAGE.
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A Molecular-Genetic Study of the Arabidopsis Toc75 Gene Family.
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Toc12, a Novel Subunit of the Intermembrane Space Preprotein Translocon of Chloroplasts.
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Inner envelope protein 32 is imported into chloroplasts by a novel pathway.
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J. Cell Sci. 117, 3975-3982
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Tic32, an Essential Component in Chloroplast Biogenesis.
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J. Biol. Chem. 279, 34756-34762
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Functional Specialization amongst the Arabidopsis Toc159 Family of Chloroplast Protein Import Receptors.
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Members of the Toc159 Import Receptor Family Represent Distinct Pathways for Protein Targeting to Plastids.
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The Protein Translocon of the Plastid Envelopes.
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J. Biol. Chem. 279, 21401-21405
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atToc159 is a selective transit peptide receptor for the import of nucleus-encoded chloroplast proteins.
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J. Cell Biol. 165, 323-334
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The Roles of Toc34 and Toc75 in Targeting the Toc159 Preprotein Receptor to Chloroplasts.
T. R. Wallas, M. D. Smith, S. Sanchez-Nieto, and D. J. Schnell (2003)
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Dimerization of Toc-GTPases at the Chloroplast Protein Import Machinery.
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The Arabidopsis ppi1 Mutant Is Specifically Defective in the Expression, Chloroplast Import, and Accumulation of Photosynthetic Proteins.
S. Kubis, A. Baldwin, R. Patel, A. Razzaq, P. Dupree, K. Lilley, J. Kurth, D. Leister, and P. Jarvis (2003)
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Proteomics of the chloroplast envelope membranes from Arabidopsis thaliana..
M. Ferro, D. Salvi, S. Brugiere, S. Miras, S. Kowalski, M. Louwagie, J. Garin, J. Joyard, and N. Rolland (2003)
Mol. Cell. Proteomics 2, 325-345
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A GTP-driven motor moves proteins across the outer envelope of chloroplasts.
E. Schleiff, M. Jelic, and J. Soll (2003)
PNAS 100, 4604-4609
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Characterization of the translocon of the outer envelope of chloroplasts.
E. Schleiff, J. Soll, M. Kuchler, W. Kuhlbrandt, and R. Harrer (2003)
J. Cell Biol. 160, 541-551
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Essential role of the G-domain in targeting of the protein import receptor atToc159 to the chloroplast outer membrane.
J. Bauer, A. Hiltbrunner, P. Weibel, P.-A. Vidi, M. Alvarez-Huerta, M. D. Smith, D. J. Schnell, and F. Kessler (2002)
J. Cell Biol. 159, 845-854
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The targeting of the atToc159 preprotein receptor to the chloroplast outer membrane is mediated by its GTPase domain and is regulated by GTP.
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J. Cell Biol. 159, 833-843
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T.-S. Yu and H.-m. Li (2001)
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Targeting of an abundant cytosolic form of the protein import receptor at Toc159 to the outer chloroplast membrane.
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D. Jackson-Constan and K. Keegstra (2001)
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Chloroplast precursor proteins compete to form early import intermediates in isolated pea chloroplasts.
P. E. Row and J. C. Gray (2001)
J. Exp. Bot. 52, 47-56
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The effect of amino acid-modifying reagents on chloroplast protein import and the formation of early import intermediates.
P. E. Row and J. C. Gray (2001)
J. Exp. Bot. 52, 57-66
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Identification of a Hsp70 Recognition Domain within the Rubisco Small Subunit Transit Peptide.
R. A. Ivey III, C. Subramanian, and B. D. Bruce (2000)
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Precursors Bind to Specific Sites on Thylakoid Membranes prior to Transport on the Delta pH Protein Translocation System.
X. Ma and K. Cline (2000)
J. Biol. Chem. 275, 10016-10022
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The Transit Sequence of Ferredoxin Contains Different Domains for Translocation across the Outer and Inner Membrane of the Chloroplast Envelope.
W. A. Rensink, D. J. Schnell, and P. J. Weisbeek (2000)
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Initial Binding of Preproteins Involving the Toc159 Receptor Can Be Bypassed during Protein Import into Chloroplasts.
K. Chen, X. Chen, and D. J. Schnell (2000)
Plant Physiology 122, 813-822
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The C Terminus of a Chloroplast Precursor Modulates Its Interaction with the Translocation Apparatus and PIRAC.
C. Dabney-Smith, P. W. J. van den Wijngaard, Y. Treece, W. J. Vredenberg, and B. D. Bruce (1999)
J. Biol. Chem. 274, 32351-32359
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GTP Promotes the Formation of Early-Import Intermediates But Is Not Required during the Translocation Step of Protein Import into Chloroplasts.
M. E. Young, K. Keegstra, and J. E. Froehlich (1999)
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Insertion of atToc34 into the Chloroplastic Outer Membrane Is Assisted by at Least Two Proteinaceous Components in the Import System.
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Protein Import and Routing Systems of Chloroplasts.
K. Keegstra and K. Cline (1999)
PLANT CELL 11, 557-570
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The evolutionary origin of the protein-translocating channel of chloroplastic envelope membranes: Identification of a cyanobacterial homolog.
S. Reumann, J. Davila-Aponte, and K. Keegstra (1999)
PNAS 96, 784-789
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Origin of a chloroplast protein importer.
B. Bolter, J. Soll, A. Schulz, S. Hinnah, and R. Wagner (1998)
PNAS 95, 15831-15836
   Abstract »    Full Text »    PDF »
Tic20 and Tic22 Are New Components of the Protein Import Apparatus at the Chloroplast Inner Envelope Membrane.
A. Kouranov, X. Chen, B. Fuks, and D. J. Schnell (1998)
J. Cell Biol. 143, 991-1002
   Abstract »    Full Text »    PDF »
Domains of a Transit Sequence Required for in Vivo Import in Arabidopsis Chloroplasts.
W. Albert Rensink, M. Pilon, and P. Weisbeek (1998)
Plant Physiology 118, 691-699
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The Hydrophilic Domain of Tic110, an Inner Envelope Membrane Component of the Chloroplastic Protein Translocation Apparatus, Faces the Stromal Compartment.
D. T. Jackson, J. E. Froehlich, and K. Keegstra (1998)
J. Biol. Chem. 273, 16583-16588
   Abstract »    Full Text »    PDF »
Positive Charges Determine the Topology and Functionality of the Transmembrane Domain in the Chloroplastic Outer Envelope Protein Toc34.
T. May and J. Soll (1998)
J. Cell Biol. 141, 895-904
   Abstract »    Full Text »    PDF »
Analysis of the Interactions of Preproteins with the Import Machinery over the Course of Protein Import into Chloroplasts.
A. Kouranov and D. J. Schnell (1997)
J. Cell Biol. 139, 1677-1685
   Abstract »    Full Text »    PDF »
Isolation and characterization of an amino acid-selective channel protein present in the chloroplastic outer envelope membrane.
K. Pohlmeyer, J. Soll, T. Steinkamp, S. Hinnah, and R. Wagner (1997)
PNAS 94, 9504-9509
   Abstract »    Full Text »    PDF »
Mitochondrial Protein Import. Tom40 PLAYS A MAJOR ROLE IN TARGETING AND TRANSLOCATION OF PREPROTEINS BY FORMING A SPECIFIC BINDING SITE FOR THE PRESEQUENCE.
D. Rapaport, W. Neupert, and R. Lill (1997)
J. Biol. Chem. 272, 18725-18731
   Abstract »    Full Text »    PDF »
A Novel Chloroplastic Outer Membrane-targeting Signal That Functions at Both Termini of Passenger Polypeptides.
H.-m. Li and L.-J. Chen (1997)
J. Biol. Chem. 272, 10968-10974
   Abstract »    Full Text »    PDF »
Identification of a Translocation Intermediate Occupying Functional Protein Import Sites in the Chloroplastic Envelope Membrane.
J. E. Froehlich and K. Keegstra (1997)
J. Biol. Chem. 272, 8077-8082
   Abstract »    Full Text »    PDF »
Identification of Protein Transport Complexes in the Chloroplastic Envelope Membranes via Chemical Cross-Linking.
M. Akita, E. Nielsen, and K. Keegstra (1997)
J. Cell Biol. 136, 983-994
   Abstract »    Full Text »    PDF »
Insertion of the 34-kDa Chloroplast Protein Import Component, IAP34, into the Chloroplast Outer Membrane Is Dependent on Its Intrinsic GTP-binding Capacity.
D. Chen and D. J. Schnell (1997)
J. Biol. Chem. 272, 6614-6620
   Abstract »    Full Text »    PDF »
The Early Stage of Chloroplast Protein Import Involves Com70.
L. Kourtz and K. Ko (1997)
J. Biol. Chem. 272, 2808-2813
   Abstract »    Full Text »    PDF »
In Vitro Interaction between a Chloroplast Transit Peptide and Chloroplast Outer Envelope Lipids Is Sequence-specific and Lipid Class-dependent.
P. Pinnaduwage and B. D. Bruce (1996)
J. Biol. Chem. 271, 32907-32915
   Abstract »    Full Text »    PDF »
Protein Translocation at the Envelope and Thylakoid Membranes of Chloroplasts.
A. Kouranov and D. J. Schnell (1996)
J. Biol. Chem. 271, 31009-31012
   Full Text »    PDF »
A Protein Import Receptor of Chloroplasts Is Inserted into the Outer Envelope Membrane by a Novel Pathway.
E. Muckel and J. Soll (1996)
J. Biol. Chem. 271, 23846-23852
   Abstract »    Full Text »    PDF »
Phosphorylation of the Transit Sequence of Chloroplast Precursor Proteins.
K. Waegemann and Jür. Soll (1996)
J. Biol. Chem. 271, 6545-6554
   Abstract »    Full Text »    PDF »
Ricin A Chain Fused to a Chloroplast-targeting Signal Is Unfolded on the Chloroplast Surface Prior to Import across the Envelope Membranes.
D. Walker, A. M. Chaddock, J. A. Chaddock, L. M. Roberts, J. M. Lord, and C. Robinson (1996)
J. Biol. Chem. 271, 4082-4085
   Abstract »    Full Text »    PDF »
Isolation and Characterization of a cDNA Clone Encoding a Member of the Com44/Cim44 Envelope Components of the Chloroplast Protein Import Apparatus.
K. Ko, D. Budd, C. Wu, F. Seibert, L. Kourtz, and Z. W. Ko (1995)
J. Biol. Chem. 270, 28601-28608
   Abstract »    Full Text »    PDF »
Toc34 is a preprotein receptor regulated by GTP and phosphorylation.
N. Sveshnikova, J. Soll, and E. Schleiff (2000)
PNAS 97, 4973-4978
   Abstract »    Full Text »    PDF »
A second, substrate-dependent site of protein import into chloroplasts.
S. Reinbothe, R. Mache, and C. Reinbothe (2000)
PNAS 97, 9795-9800
   Abstract »    Full Text »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)