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Science 23 December 1994:
Vol. 266. no. 5193, pp. 1986 - 1988
DOI: 10.1126/science.7528444
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Articles

Science, Vol 266, Issue 5193, 1986-1988
Copyright © 1994 by American Association for the Advancement of Science

articles

Splicing of the rolA transcript of Agrobacterium rhizogenes in Arabidopsis

A Magrelli, K Langenkemper, C Dehio, J Schell, and A Spena

Max-Plank-Institut fur Zuchtungsforschung, Cologne, Germany.

The rolA gene encoded on the Ri plasmid A4 of Agrobacterium rhizogenes is one of the transferred (TL-DNA) genes involved in the pathogenesis of hairy-root disease in plants. The function of the 100-amino acid protein product of rolA is unknown, although its expression causes physiological and developmental alterations in transgenic plants. The rolA gene of A. rhizogenes contains an intron in its untranslated leader region that has features typical of plant pre-messenger RNA introns. Transcription and splicing of the rolA pre-messenger RNA occur in the plant cell.


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